Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOK39_RS15310 Genome accession   NZ_HG992337
Coordinates   3603222..3604955 (-) Length   577 a.a.
NCBI ID   WP_228599917.1    Uniprot ID   A0AAU9I0Y6
Organism   Xanthomonas arboricola strain 1314c isolate 1314c     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3598222..3609955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOK39_RS15300 (XA1314C_30130) - 3599559..3601172 (+) 1614 WP_039525813.1 HAMP domain-containing sensor histidine kinase -
  LOK39_RS15305 (XA1314C_30140) pilR 3601365..3602759 (+) 1395 WP_047127086.1 sigma-54 dependent transcriptional regulator Regulator
  LOK39_RS15310 (XA1314C_30150) pilB 3603222..3604955 (-) 1734 WP_228599917.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOK39_RS15315 (XA1314C_30160) pilA 3605093..3605521 (-) 429 WP_104593849.1 pilin Machinery gene
  LOK39_RS15320 (XA1314C_30170) pilC 3605861..3607123 (+) 1263 WP_104593888.1 type II secretion system F family protein Machinery gene
  LOK39_RS15325 (XA1314C_30180) - 3607130..3607993 (+) 864 WP_002812278.1 A24 family peptidase -
  LOK39_RS15330 (XA1314C_30190) coaE 3608007..3608630 (+) 624 WP_039525803.1 dephospho-CoA kinase -
  LOK39_RS15335 (XA1314C_30200) - 3608730..3608885 (+) 156 Protein_3006 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62525.65 Da        Isoelectric Point: 5.6006

>NTDB_id=1112315 LOK39_RS15310 WP_228599917.1 3603222..3604955(-) (pilB) [Xanthomonas arboricola strain 1314c isolate 1314c]
MSAVAANLVGITGIARRLVQDGALDEISARTAMDQAAAAKIPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSVFDASQ
NAIKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLGD
DDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKL
FLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPE
HALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALVK
AAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1112315 LOK39_RS15310 WP_228599917.1 3603222..3604955(-) (pilB) [Xanthomonas arboricola strain 1314c isolate 1314c]
ATGAGTGCAGTCGCGGCGAACTTAGTAGGAATTACAGGCATTGCACGCCGCTTGGTGCAAGACGGAGCACTGGACGAAAT
AAGTGCCCGTACCGCGATGGATCAAGCTGCGGCGGCGAAAATTCCGCTTCCTCAATGGTTCTCCGAGAAGAAGCTGGTTT
CAGCGTCTCAACTCGCCGCCGCCAACGCGGTCGAGTTTGGCATGCCACTGATGGATGTGTCGGTATTCGACGCCAGCCAA
AACGCCATTAAGCTAGTCAGTGAAGAGTTGCTCCAGAAGTATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTT
CGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAACCTGGTGGTCGAGCCGATCC
TGGTCGACGAGGACCAGATCCGTCGTACCTTGGAGCAGTGGCAGGCCAGCAACGCATCGTTGGGCTCGTCGCTCGGTGAC
GACGATGAGGGGATGGGCGATCTGGACGTGTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGC
CAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCC
ACTTCGAGCCCTATGAAGACGACTATCGGGTGCGCTTGCGGATCGATGGGCTGCTGAAGAATGTGGCCAAGGCACCGGTG
AAGCTGAACCAGCGTATCGCGGCACGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGG
GCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACATTGTTCGGCGAAAAGG
TAGTGTTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTG
TTCCTGGAGGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTA
CACTGCACTGGGCATCCTCAACGACGAAACGCGCAACATCTCCACCGCCGAGGACCCGGTGGAAATCCGGTTGCCCGGCG
TCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTTCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCATATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATCGCGCCGTACAACATCACCT
CGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCCGAG
CATGCGTTGCTGGCGGAAGGCTTCACGCCGGCACAGATCGCTGCCGGTATCGAGTTGTATGAGGCGGTCGGTTGCGACGA
GTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGG
AAGGCGGTAACGCGATGCAGATTGCCGAGGCGGCACAGAGTATCGGCATCCGCGACCTGCGCCAGTCGGCGTTGGTCAAG
GCCGCGCACGGGGTGACCAGCTTGGCCGAGATCAACCGGGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.44

0.551
  pilB Acinetobacter baylyi ADP1

55.458

98.44

0.546
  pilB Legionella pneumophila strain ERS1305867

52.34

100

0.523
  pilF Neisseria gonorrhoeae MS11

49.12

98.44

0.484
  pilB Vibrio cholerae strain A1552

50.273

95.147

0.478
  pilB Vibrio campbellii strain DS40M4

45.87

98.614

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

46.607

97.054

0.452
  pilF Thermus thermophilus HB27

40.192

90.121

0.362

Multiple sequence alignment