Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACMCNG_RS06450 Genome accession   NZ_AP038785
Coordinates   1449360..1449797 (+) Length   145 a.a.
NCBI ID   WP_226691633.1    Uniprot ID   -
Organism   Rodentibacter sp. THUN1654     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1444360..1454797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMCNG_RS06425 (THUN1654_12340) tadA 1445155..1445634 (-) 480 WP_226692921.1 tRNA adenosine(34) deaminase TadA -
  ACMCNG_RS06430 (THUN1654_12350) - 1445673..1446524 (-) 852 WP_226691630.1 thymidylate synthase -
  ACMCNG_RS06435 (THUN1654_12360) lgt 1446648..1447448 (-) 801 WP_374707287.1 prolipoprotein diacylglyceryl transferase -
  ACMCNG_RS06440 (THUN1654_12370) rppH 1447580..1448164 (-) 585 WP_226691631.1 RNA pyrophosphohydrolase -
  ACMCNG_RS06445 (THUN1654_12380) ampD 1448681..1449244 (-) 564 WP_226691632.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMCNG_RS06450 (THUN1654_12390) pilA 1449360..1449797 (+) 438 WP_226691633.1 prepilin peptidase-dependent pilin Machinery gene
  ACMCNG_RS06455 (THUN1654_12400) pilB 1449794..1451173 (+) 1380 WP_226691634.1 GspE/PulE family protein Machinery gene
  ACMCNG_RS06460 (THUN1654_12410) pilC 1451175..1452395 (+) 1221 WP_226691635.1 type II secretion system F family protein Machinery gene
  ACMCNG_RS06465 (THUN1654_12420) pilD 1452392..1453093 (+) 702 WP_226691636.1 prepilin peptidase Machinery gene
  ACMCNG_RS06470 (THUN1654_12430) coaE 1453093..1453713 (+) 621 WP_226692923.1 dephospho-CoA kinase -
  ACMCNG_RS06475 (THUN1654_12440) yacG 1453706..1453912 (+) 207 WP_226691637.1 DNA gyrase inhibitor YacG -
  ACMCNG_RS06480 (THUN1654_12450) - 1453909..1454190 (+) 282 WP_226691638.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15020.27 Da        Isoelectric Point: 8.4769

>NTDB_id=111179 ACMCNG_RS06450 WP_226691633.1 1449360..1449797(+) (pilA) [Rodentibacter sp. THUN1654]
MKLTSQRTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAMSELLQAATPFKSDVELCVYSTGATANCSGGSNGIA
ADITSPKGYVKGISTNAGVITVVGNGALDGVQYTLTATGDHSRGISWSTTCNGDTSLFPAGFCTK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=111179 ACMCNG_RS06450 WP_226691633.1 1449360..1449797(+) (pilA) [Rodentibacter sp. THUN1654]
ATGAAACTCACCTCTCAACGCACATTGAAAAAAGGATTTACACTAATCGAATTAATGATCGTGATAGCCATCATCGCGAT
TCTCGCGACAATTGCAATTCCCTCTTATCAGAATTATACAAAAAAGGCGGCGATGTCTGAATTGTTACAAGCCGCAACCC
CTTTTAAATCCGACGTGGAACTCTGCGTTTATAGCACAGGAGCAACCGCAAATTGCAGCGGCGGTTCAAACGGTATCGCT
GCCGATATTACCTCACCAAAAGGCTATGTGAAAGGTATTAGTACTAACGCCGGCGTTATTACTGTTGTCGGCAACGGCGC
TTTAGACGGTGTCCAATATACATTAACCGCCACCGGTGATCATTCTAGAGGAATAAGCTGGTCTACAACCTGTAACGGTG
ATACCTCCTTATTTCCGGCAGGATTCTGTACAAAATGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

72.222

99.31

0.717
  pilA Haemophilus influenzae Rd KW20

69.444

99.31

0.69
  pilA Glaesserella parasuis strain SC1401

57.746

97.931

0.566
  pilA Vibrio campbellii strain DS40M4

38.71

100

0.414

Multiple sequence alignment