Detailed information NTG1111734

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HMPREF9425_RS02235
• Genome accession: NZ_GL831112
• Coordinates: 420630..421325 (+)
• Length: 231 a.a.
• NCBI ID: WP_003093743.1
• Uniprot ID: A0AAW7QFH2
• Organism: Streptococcus vestibularis ATCC 49124
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 415630..426325

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMPREF9425_RS02210 (HMPREF9425_0462)	-	416726..417235 (+)	510	WP_037621639.1	hypothetical protein	-
HMPREF9425_RS10655	-	417447..417566 (+)	120	Protein_446	IS30 family transposase	-
HMPREF9425_RS02215 (HMPREF9425_0463)	-	417616..417885 (-)	270	WP_003096419.1	GIY-YIG nuclease family protein	-
HMPREF9425_RS02220 (HMPREF9425_0464)	-	417889..418653 (-)	765	WP_003096421.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
HMPREF9425_RS02225 (HMPREF9425_0465)	-	418703..419638 (-)	936	WP_003096423.1	polysaccharide deacetylase family protein	-
HMPREF9425_RS02230 (HMPREF9425_0466)	-	419768..420523 (+)	756	WP_003096426.1	lysophospholipid acyltransferase family protein	-
HMPREF9425_RS02235 (HMPREF9425_0467)	comEA	420630..421325 (+)	696	WP_003093743.1	helix-hairpin-helix domain-containing protein	Machinery gene
HMPREF9425_RS02240 (HMPREF9425_0468)	-	421315..423555 (+)	2241	WP_003096429.1	DNA internalization-related competence protein ComEC/Rec2	-
HMPREF9425_RS02245 (HMPREF9425_0469)	-	423641..424903 (+)	1263	WP_003093180.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
HMPREF9425_RS02250 (HMPREF9425_0470)	-	424969..425514 (+)	546	WP_003093731.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 231 a.a.
• Molecular weight: 24588.56 Da
• Isoelectric Point: 4.7187

>NTDB_id=1111734 HMPREF9425_RS02235 WP_003093743.1 420630..421325(+) (comEA) [Streptococcus vestibularis ATCC 49124]
MKEKIIDYVNNNRLFVSIIGVLMMVFCFFLWMTCGAGNSMEAETSYTDVTTLSTSSSKEGSKSLTEVSSQSQTEGSEKVK
SKVTVDVKGAVVKPGVYTLKVSARVTDAIQEAGGMTEDADAKSVNLAASLSDEEVIYVANKDENVSVLDQSDTGQVSNKG
GQAVSKNGKINLNTATSEQLQTISGIGAKRAEDIVAYRESHGGFQSVGDLKNVSGIGDKTLDKIRESIYVA

Nucleotide

• Length: 696 bp

>NTDB_id=1111734 HMPREF9425_RS02235 WP_003093743.1 420630..421325(+) (comEA) [Streptococcus vestibularis ATCC 49124]
GTGAAGGAAAAGATTATAGACTATGTTAACAATAATCGTCTATTTGTGAGTATCATTGGTGTGCTGATGATGGTTTTCTG
CTTCTTTCTGTGGATGACTTGTGGTGCCGGCAACAGCATGGAGGCAGAGACGTCTTATACAGATGTGACAACATTGTCAA
CCTCCTCATCCAAAGAAGGGTCAAAATCACTTACGGAAGTGTCTTCTCAGTCACAAACTGAAGGAAGTGAAAAAGTTAAG
TCAAAAGTAACGGTAGATGTTAAGGGGGCTGTGGTCAAGCCAGGTGTATATACGCTAAAAGTAAGCGCTAGGGTGACAGA
TGCCATTCAGGAAGCTGGAGGAATGACAGAGGATGCAGACGCTAAGAGTGTTAATTTGGCCGCAAGCTTGTCAGATGAAG
AGGTTATTTATGTAGCCAATAAAGACGAGAATGTTTCTGTTCTTGATCAATCAGATACTGGTCAGGTCTCTAACAAAGGA
GGGCAGGCTGTATCTAAGAATGGCAAAATTAACTTAAATACAGCGACCTCAGAGCAGTTACAAACTATTTCTGGAATTGG
TGCCAAGAGGGCAGAGGATATTGTCGCTTATCGTGAAAGTCATGGCGGCTTTCAGTCCGTAGGTGACCTGAAAAATGTTT
CAGGCATTGGTGATAAAACGTTAGATAAAATCAGAGAGTCCATCTATGTGGCTTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (85-138)

• Helix-hairpin-helix motif (HHH_3) (166-228)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	88.312	100	0.883
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	40.175	99.134	0.398
comEA/celA/cilE	Streptococcus pneumoniae D39	39.056	100	0.394
comEA/celA/cilE	Streptococcus pneumoniae Rx1	39.056	100	0.394
comEA/celA/cilE	Streptococcus pneumoniae R6	39.056	100	0.394
comEA/celA/cilE	Streptococcus mitis SK321	39.301	99.134	0.39
comEA	Latilactobacillus sakei subsp. sakei 23K	35.246	100	0.372
comEA/celA/cilE	Streptococcus mitis NCTC 12261	37.281	98.701	0.368