Detailed information NTG1110564

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACOC81_RS14645
• Genome accession: NZ_CP184649
• Coordinates: 3233486..3234652 (-)
• Length: 388 a.a.
• NCBI ID: WP_122445675.1
• Uniprot ID: -
• Organism: Pseudomonas viridiflava strain p21.B10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3228486..3239652

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOC81_RS14630 (ACOC81_14630)	-	3229649..3231061 (+)	1413	WP_122445673.1	sigma-54 interaction domain-containing protein	-
ACOC81_RS14635 (ACOC81_14635)	-	3231313..3232704 (+)	1392	WP_122445674.1	GntP family permease	-
ACOC81_RS14640 (ACOC81_14640)	-	3232715..3233473 (+)	759	WP_004879569.1	3-hydroxybutyrate dehydrogenase	-
ACOC81_RS14645 (ACOC81_14645)	pilU	3233486..3234652 (-)	1167	WP_122445675.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACOC81_RS14650 (ACOC81_14650)	-	3234764..3235039 (+)	276	WP_004879573.1	peptidylprolyl isomerase	-
ACOC81_RS14655 (ACOC81_14655)	pgm	3235094..3236740 (-)	1647	WP_122445676.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
ACOC81_RS14660 (ACOC81_14660)	-	3236847..3239117 (-)	2271	WP_122445677.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 42745.07 Da
• Isoelectric Point: 6.6698

>NTDB_id=1110564 ACOC81_RS14645 WP_122445675.1 3233486..3234652(-) (pilU) [Pseudomonas viridiflava strain p21.B10]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGTETLKPGEVAKIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLYLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNQLNELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKHADSQNNVRLRLKLHGENGVNTLTTAPAAPTGAGKPSMVEWGLVDDEPPSQS

Nucleotide

• Length: 1167 bp

>NTDB_id=1110564 ACOC81_RS14645 WP_122445675.1 3233486..3234652(-) (pilU) [Pseudomonas viridiflava strain p21.B10]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTATCTTTCCACCGGAGCGCCGCCCTG
CGCCAAGTTCAATGGCGTGCTCAAGCCACTGGGCACCGAAACCCTCAAGCCGGGCGAAGTGGCGAAGATCGCTCAGGGCC
TGATGGACGAAGAGCAGAAGCTGGAATTCCTGCGCGAACTGGAAATGAACCTCGCCGTTTCACTGGCGGGCATCGGGCGT
TTCCGGATCAACATCTTCATGCAGCGCAACGAAGTATCGATTGTCGCGCGTAACATCAAGCTGGATATTCCGCGCTTCGA
AGACCTGTATCTGCCGCCGGTTCTGCTCGATGTGATCATGGAAAAGCACGGCCTGGTGCTGTTCGTCGGTGCCACCGGTT
CGGGTAAGTCCACCTCATTGGCGGCGCTGATCGACTACCGCAACCGCAACGCCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAATTCATTCATCGACACAAGAAGTCCATCGTCAATCAGCGGGAAGTGGGCGTCGATACCCGCAGTTTCAGGGC
CGCGCTGAAAAACACCCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCACG
CGCTGGCATTCGCCGACACCGGGCACCTGGCGATCTCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAACGACGTCCGCAATTGCTGCATGACCTGGGCAACAACCTCAAGGCGTTCGTGTCGCAGCG
TCTGGTCAAAACCCCGGATGGCAAGCGCCGTGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGTGATCTGATCC
AGCGTAACCAGTTGAACGAGCTCAAAGGGATCATGGAGAAGTCCGGCAGCCTCGGCATGCAGACCTTCGACACTGCGTTG
TTCAATCTAGCGGTGGAGGGCGCTATCAGCGAAGAGGAAGCGCTGAAACATGCGGATTCGCAGAACAACGTGCGCCTGCG
CCTCAAGTTGCACGGCGAAAACGGTGTGAACACCCTGACGACAGCGCCAGCTGCGCCGACCGGCGCGGGCAAGCCGAGCA
TGGTCGAGTGGGGGTTGGTCGACGACGAACCCCCGTCCCAGAGTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.065	94.845	0.541
pilU	Acinetobacter baylyi ADP1	51.831	91.495	0.474
pilU	Vibrio cholerae strain A1552	51.143	90.206	0.461
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.51	94.588	0.374
pilT	Pseudomonas aeruginosa PAK	41.108	88.402	0.363
pilT	Pseudomonas stutzeri DSM 10701	40.816	88.402	0.361