Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACKTSO_RS08070 Genome accession   NZ_AP035889
Coordinates   1660573..1661982 (-) Length   469 a.a.
NCBI ID   WP_021461531.1    Uniprot ID   U1H1C7
Organism   Gallibacterium anatis strain SMUHGa01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1655573..1666982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKTSO_RS08035 (SMUG_15760) - 1655857..1656516 (+) 660 WP_407902308.1 formate dehydrogenase subunit gamma -
  ACKTSO_RS08040 (SMUG_15770) fdhE 1656518..1657441 (+) 924 WP_013746614.1 formate dehydrogenase accessory protein FdhE -
  ACKTSO_RS08045 (SMUG_15780) - 1657507..1657779 (-) 273 WP_039084192.1 GNAT family N-acetyltransferase -
  ACKTSO_RS08050 (SMUG_15790) yacG 1657865..1658083 (-) 219 WP_018345915.1 DNA gyrase inhibitor YacG -
  ACKTSO_RS08055 (SMUG_15800) coaE 1658073..1658705 (-) 633 WP_407902309.1 dephospho-CoA kinase -
  ACKTSO_RS08060 (SMUG_15810) - 1658720..1659352 (-) 633 WP_407902310.1 prepilin peptidase -
  ACKTSO_RS08065 (SMUG_15820) - 1659349..1660557 (-) 1209 WP_285095951.1 type II secretion system F family protein -
  ACKTSO_RS08070 (SMUG_15830) pilB 1660573..1661982 (-) 1410 WP_021461531.1 GspE/PulE family protein Machinery gene
  ACKTSO_RS08075 (SMUG_15840) pilA 1661999..1662460 (-) 462 WP_407902311.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACKTSO_RS08080 (SMUG_15850) ampD 1662565..1663119 (+) 555 WP_407902312.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACKTSO_RS08085 (SMUG_15860) - 1663206..1663541 (+) 336 WP_013746603.1 hypothetical protein -
  ACKTSO_RS08090 (SMUG_15870) - 1663650..1665044 (+) 1395 WP_407902313.1 chloride channel protein -
  ACKTSO_RS08095 (SMUG_15880) dusA 1665025..1666005 (+) 981 WP_407902314.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52878.32 Da        Isoelectric Point: 5.3117

>NTDB_id=110895 ACKTSO_RS08070 WP_021461531.1 1660573..1661982(-) (pilB) [Gallibacterium anatis strain SMUHGa01]
MGYCVKALDSEQLFEISELQWQKNQQQYELLLRYLAVPLQEQAQQLWLAVESFDNLAACETFAFLSGKQIEPVAIGQTAL
KQLLQQLQPGTATNQVQESQLHSYQQNDIVAKSDEIAEVDAQEPIIQLFNQLLESGLQYSVSDIHLEPLRQEFQIRFRID
GVLQRHQVLTLSIGQRLNSRLKLLAKLDINELRLPQDGRFRFKTAFGETLDIRLSSLPTQYGEKIVLRLQPNTPVFDDFT
ELGMTNEQAMLFRQALSQPQGLILVTGPTGSGKSLTLYSGLHFLNQESRHILTAEDPIELQLNGIIQTQANAAIGLTFAT
LLRSFLRQDPDVIMVGEIRDQETAEIALRAAQTGHLVLSTLHTNTAIGAISRLQQLGIAQHELENSLLLVVAQRLVRKRC
TLCQGDKSQQCDCVDGYRGRTGVYQLLHYQQQQIQMDYPTLLAAAQLKLQQRITTTAEVERVFGKTENS

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=110895 ACKTSO_RS08070 WP_021461531.1 1660573..1661982(-) (pilB) [Gallibacterium anatis strain SMUHGa01]
ATGGGCTATTGTGTAAAGGCATTGGATTCGGAGCAGCTGTTTGAAATTTCAGAATTGCAATGGCAGAAGAATCAACAGCA
ATATGAACTGTTATTGCGTTATTTAGCTGTACCGTTGCAGGAACAAGCGCAACAGTTATGGTTAGCGGTTGAGTCTTTCG
ATAATTTAGCAGCTTGCGAGACCTTTGCTTTTCTTTCCGGCAAACAGATTGAGCCGGTTGCGATCGGACAAACTGCACTG
AAACAGTTATTACAGCAGCTGCAACCCGGAACGGCGACAAATCAAGTGCAAGAATCACAGCTACATAGTTATCAGCAAAA
TGATATTGTCGCAAAATCGGATGAAATTGCGGAAGTTGATGCACAAGAGCCGATTATTCAACTATTCAATCAATTATTGG
AATCAGGTTTGCAATATTCCGTTTCTGATATTCATCTTGAGCCGTTACGTCAGGAGTTCCAGATCCGCTTTAGAATTGAC
GGCGTATTACAACGGCATCAGGTGCTGACGTTGTCAATAGGTCAACGCTTAAACTCACGCCTAAAATTGTTGGCAAAACT
GGATATTAACGAATTACGCTTGCCGCAGGATGGTCGTTTTCGTTTTAAAACCGCATTCGGTGAAACATTAGATATTCGGT
TATCTTCTCTACCGACACAATATGGTGAAAAAATCGTATTACGATTGCAACCTAACACACCGGTGTTTGATGATTTTACC
GAACTGGGAATGACAAACGAGCAGGCTATGCTGTTCCGGCAAGCGTTATCTCAACCGCAAGGATTAATCTTGGTTACCGG
ACCGACCGGAAGTGGTAAAAGTTTGACATTATATAGCGGTTTACATTTTTTAAATCAGGAAAGTCGCCACATTCTTACGG
CGGAAGATCCGATAGAACTGCAATTAAACGGCATTATTCAGACTCAAGCAAATGCCGCAATCGGTTTAACTTTTGCAACA
TTATTGCGGAGTTTTTTACGCCAAGATCCGGATGTGATTATGGTTGGGGAAATTCGAGATCAGGAAACGGCAGAGATAGC
ATTACGCGCGGCACAGACGGGACATTTAGTGTTATCGACACTGCATACCAATACGGCAATAGGTGCAATTTCCCGATTAC
AGCAGTTGGGAATTGCGCAACACGAATTGGAAAATTCATTATTGTTGGTGGTGGCACAACGTTTAGTCCGCAAACGTTGC
ACCTTATGCCAAGGTGATAAATCTCAGCAATGCGATTGTGTTGATGGTTATCGCGGACGAACCGGCGTTTATCAACTGCT
ACATTATCAGCAACAACAAATTCAAATGGATTATCCGACATTATTGGCGGCAGCACAGCTGAAATTACAGCAACGGATCA
CGACAACAGCAGAGGTTGAACGCGTCTTCGGCAAGACGGAGAACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(131-398)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-91)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U1H1C7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

59.43

97.228

0.578
  pilB Haemophilus influenzae Rd KW20

59.43

97.228

0.578
  pilB Glaesserella parasuis strain SC1401

53.632

99.787

0.535
  pilB Vibrio parahaemolyticus RIMD 2210633

38.677

100

0.412
  pilB Vibrio cholerae strain A1552

39.834

100

0.409
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.407
  pilB Legionella pneumophila strain ERS1305867

36.157

100

0.373
  pilB Acinetobacter baylyi ADP1

39.503

94.456

0.373
  pilB Acinetobacter baumannii D1279779

39.13

93.177

0.365

Multiple sequence alignment