Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACKTSO_RS08075 Genome accession   NZ_AP035889
Coordinates   1661999..1662460 (-) Length   153 a.a.
NCBI ID   WP_407902311.1    Uniprot ID   -
Organism   Gallibacterium anatis strain SMUHGa01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1656999..1667460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKTSO_RS08045 (SMUG_15780) - 1657507..1657779 (-) 273 WP_039084192.1 GNAT family N-acetyltransferase -
  ACKTSO_RS08050 (SMUG_15790) yacG 1657865..1658083 (-) 219 WP_018345915.1 DNA gyrase inhibitor YacG -
  ACKTSO_RS08055 (SMUG_15800) coaE 1658073..1658705 (-) 633 WP_407902309.1 dephospho-CoA kinase -
  ACKTSO_RS08060 (SMUG_15810) - 1658720..1659352 (-) 633 WP_407902310.1 prepilin peptidase -
  ACKTSO_RS08065 (SMUG_15820) - 1659349..1660557 (-) 1209 WP_285095951.1 type II secretion system F family protein -
  ACKTSO_RS08070 (SMUG_15830) pilB 1660573..1661982 (-) 1410 WP_021461531.1 GspE/PulE family protein Machinery gene
  ACKTSO_RS08075 (SMUG_15840) pilA 1661999..1662460 (-) 462 WP_407902311.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACKTSO_RS08080 (SMUG_15850) ampD 1662565..1663119 (+) 555 WP_407902312.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACKTSO_RS08085 (SMUG_15860) - 1663206..1663541 (+) 336 WP_013746603.1 hypothetical protein -
  ACKTSO_RS08090 (SMUG_15870) - 1663650..1665044 (+) 1395 WP_407902313.1 chloride channel protein -
  ACKTSO_RS08095 (SMUG_15880) dusA 1665025..1666005 (+) 981 WP_407902314.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 16477.89 Da        Isoelectric Point: 8.4502

>NTDB_id=110896 ACKTSO_RS08075 WP_407902311.1 1661999..1662460(-) (pilA) [Gallibacterium anatis strain SMUHGa01]
MKKREQLKSNYAGFTLIELMIVIAIIAVLATIAIPSYQNYTKKAAISELIQASSPYKAEVELCIYNTKSKASCNAGQNGI
QAKPSNMDKFKYLKEVNVAAGVISVFGKSSLENISYKMTPSDGNNGELIDWSVTCSGDNGLFPAGFCSNTSAN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=110896 ACKTSO_RS08075 WP_407902311.1 1661999..1662460(-) (pilA) [Gallibacterium anatis strain SMUHGa01]
ATGAAAAAACGTGAACAACTTAAGTCGAATTATGCCGGATTTACTTTAATCGAATTGATGATCGTCATTGCGATTATTGC
CGTGTTGGCAACCATTGCCATTCCCTCTTATCAAAATTACACCAAAAAGGCGGCAATATCAGAATTGATTCAGGCCTCTT
CACCCTATAAGGCGGAAGTTGAACTCTGTATTTATAACACTAAGAGTAAAGCAAGCTGTAACGCCGGTCAAAACGGCATT
CAGGCGAAGCCGTCTAATATGGATAAATTTAAATATCTTAAAGAGGTTAATGTGGCTGCCGGTGTTATTTCTGTTTTCGG
TAAATCCAGTCTGGAGAACATCAGTTATAAGATGACACCAAGTGACGGCAATAACGGTGAATTAATTGATTGGAGTGTAA
CTTGTAGCGGTGATAATGGGCTGTTTCCGGCAGGATTTTGTAGCAATACTTCTGCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

53.333

98.039

0.523
  pilA Haemophilus influenzae Rd KW20

52.667

98.039

0.516
  pilA Haemophilus influenzae 86-028NP

56.618

88.889

0.503

Multiple sequence alignment