Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACN9KP_RS12495 Genome accession   NZ_CP183947
Coordinates   2567387..2568001 (-) Length   204 a.a.
NCBI ID   WP_003152867.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ya-1 isolate Tropical rainforest soil     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2562387..2573001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN9KP_RS12465 (ACN9KP_12465) rpsT 2562687..2562953 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  ACN9KP_RS12470 (ACN9KP_12470) holA 2562970..2564011 (-) 1042 Protein_2409 DNA polymerase III subunit delta -
  ACN9KP_RS12475 (ACN9KP_12475) - 2564051..2564205 (-) 155 Protein_2410 hypothetical protein -
  ACN9KP_RS12480 (ACN9KP_12480) - 2564246..2564380 (+) 135 WP_003152870.1 YqzM family protein -
  ACN9KP_RS12485 (ACN9KP_12485) comEC 2564399..2566750 (-) 2352 WP_283002419.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACN9KP_RS12490 (ACN9KP_12490) - 2566751..2567320 (-) 570 WP_003152868.1 ComE operon protein 2 -
  ACN9KP_RS12495 (ACN9KP_12495) comEA 2567387..2568001 (-) 615 WP_003152867.1 helix-hairpin-helix domain-containing protein Machinery gene
  ACN9KP_RS12500 (ACN9KP_12500) comER 2568060..2568881 (+) 822 WP_014305449.1 late competence protein ComER -
  ACN9KP_RS12505 (ACN9KP_12505) - 2568950..2569687 (-) 738 WP_014305450.1 class I SAM-dependent DNA methyltransferase -
  ACN9KP_RS12510 (ACN9KP_12510) rsfS 2569684..2570040 (-) 357 WP_003152864.1 ribosome silencing factor -
  ACN9KP_RS12515 (ACN9KP_12515) yqeK 2570037..2570618 (-) 582 WP_014305452.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACN9KP_RS12520 (ACN9KP_12520) - 2570608..2571177 (-) 570 WP_160249251.1 nicotinate-nucleotide adenylyltransferase -
  ACN9KP_RS12525 (ACN9KP_12525) yhbY 2571188..2571478 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  ACN9KP_RS12530 (ACN9KP_12530) aroE 2571472..2572314 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22269.48 Da        Isoelectric Point: 6.4940

>NTDB_id=1107325 ACN9KP_RS12495 WP_003152867.1 2567387..2568001(-) (comEA) [Bacillus velezensis strain Ya-1 isolate Tropical rainforest soil]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1107325 ACN9KP_RS12495 WP_003152867.1 2567387..2568001(-) (comEA) [Bacillus velezensis strain Ya-1 isolate Tropical rainforest soil]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

59.375

94.118

0.559
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

36.019

100

0.373
  comEA/celA/cilE Streptococcus mitis SK321

35.545

100

0.368
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363
  comEA/celA/cilE Streptococcus pneumoniae R6

35.749

100

0.363
  comEA/celA/cilE Streptococcus pneumoniae D39

35.749

100

0.363
  comEA/celA/cilE Streptococcus mitis NCTC 12261

35.749

100

0.363
  comEA/celA/cilE Streptococcus pneumoniae Rx1

35.749

100

0.363

Multiple sequence alignment