Detailed information NTG1107251

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACM5XX_RS12620
• Genome accession: NZ_CP183946
• Coordinates: 2571542..2572156 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain HKSSLJEBR3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2566542..2577156

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM5XX_RS12590 (ACM5XX_12590)	rpsT	2566842..2567108 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACM5XX_RS12595 (ACM5XX_12595)	holA	2567125..2568166 (-)	1042	Protein_2431	DNA polymerase III subunit delta	-
ACM5XX_RS12600 (ACM5XX_12600)	-	2568206..2568360 (-)	155	Protein_2432	hypothetical protein	-
ACM5XX_RS12605 (ACM5XX_12605)	-	2568401..2568535 (+)	135	WP_003152870.1	YqzM family protein	-
ACM5XX_RS12610 (ACM5XX_12610)	comEC	2568554..2570905 (-)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACM5XX_RS12615 (ACM5XX_12615)	-	2570906..2571475 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACM5XX_RS12620 (ACM5XX_12620)	comEA	2571542..2572156 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACM5XX_RS12625 (ACM5XX_12625)	comER	2572215..2573036 (+)	822	WP_014305449.1	late competence protein ComER	-
ACM5XX_RS12630 (ACM5XX_12630)	-	2573105..2573842 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
ACM5XX_RS12635 (ACM5XX_12635)	rsfS	2573839..2574195 (-)	357	WP_014305451.1	ribosome silencing factor	-
ACM5XX_RS12640 (ACM5XX_12640)	yqeK	2574192..2574773 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACM5XX_RS12645 (ACM5XX_12645)	-	2574763..2575332 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
ACM5XX_RS12650 (ACM5XX_12650)	yhbY	2575343..2575633 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACM5XX_RS12655 (ACM5XX_12655)	aroE	2575627..2576469 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=1107251 ACM5XX_RS12620 WP_003152867.1 2571542..2572156(-) (comEA) [Bacillus velezensis strain HKSSLJEBR3]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1107251 ACM5XX_RS12620 WP_003152867.1 2571542..2572156(-) (comEA) [Bacillus velezensis strain HKSSLJEBR3]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363