Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HZ99_RS04645 Genome accession   NZ_CP008896
Coordinates   1125131..1126579 (-) Length   482 a.a.
NCBI ID   WP_080727769.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain UK4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1120131..1131579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ99_RS04610 gspM 1120488..1120919 (-) 432 WP_080727661.1 type II secretion system protein GspM -
  HZ99_RS04615 - 1120916..1122025 (-) 1110 WP_051903045.1 GspL/Epsl periplasmic domain-containing protein -
  HZ99_RS04620 (HZ99_04765) - 1122040..1122630 (-) 591 WP_038441606.1 PulJ/GspJ family protein -
  HZ99_RS04625 (HZ99_04770) - 1122627..1122992 (-) 366 WP_038441607.1 type II secretion system protein -
  HZ99_RS04630 (HZ99_04775) gspH 1122989..1123438 (-) 450 WP_038441608.1 type II secretion system minor pseudopilin GspH -
  HZ99_RS04635 (HZ99_04780) gspG 1123441..1123899 (-) 459 WP_404942426.1 type II secretion system major pseudopilin GspG -
  HZ99_RS04640 (HZ99_04785) pilC 1123929..1125131 (-) 1203 WP_038441609.1 type II secretion system inner membrane protein GspF Machinery gene
  HZ99_RS04645 (HZ99_04790) pilB 1125131..1126579 (-) 1449 WP_080727769.1 GspE/PulE family protein Machinery gene
  HZ99_RS04650 (HZ99_04795) gspD 1126585..1128534 (-) 1950 WP_115284177.1 type II secretion system secretin GspD -
  HZ99_RS04655 (HZ99_04800) - 1128542..1128970 (-) 429 WP_038441610.1 pilus assembly protein PilZ -
  HZ99_RS04660 (HZ99_04805) - 1128967..1130619 (-) 1653 WP_038441611.1 lipoprotein UxpA -

Sequence

Protein


Download         Length: 482 a.a.        Molecular weight: 52598.28 Da        Isoelectric Point: 5.4832

>NTDB_id=110723 HZ99_RS04645 WP_080727769.1 1125131..1126579(-) (pilB) [Pseudomonas fluorescens strain UK4]
MLPYRMARQAGLARAPGPQGWTLYLRDDVDTDALQEVLRVHGLPTLVEHLDPAQYELRLGALYQAGESATAALIEGIGEQ
VDLDSLINELPRIEDLLESDNEAPVIRLINGLFAEGLRLRASDIHIETFEQSLVVRLRVDGHLREILRPPRALSAMLVSR
IKVMARLDIAEKRQPQDGRITLRSGGREVDIRVSTLPGIHGERVVMRVLDKQANLLDLNNLGMPAEVEQALRACLSRPNG
ILLSTGPTGSGKTTTLYASLNALNDGSCNILTVEDPVEYAITGIGQTAINPRAGMTFASGLRAILRQDPDVIMLGEIRDQ
ETAQIAVQASLTGHLVLSTLHTNSAVGAVTRLRDMGIEPFLIASSLKGVMAQRLVRRLCHCATPAPLQAAERQLWPELAT
LAQSFHPQGCDDCQGSGYHGRIGLYEFITLDAGLIGLLYDGASEATMHEYLADKRQSLTAMATECLRRGDTSLEQVLRAV
RD

Nucleotide


Download         Length: 1449 bp        

>NTDB_id=110723 HZ99_RS04645 WP_080727769.1 1125131..1126579(-) (pilB) [Pseudomonas fluorescens strain UK4]
TTGCTGCCTTACCGAATGGCGCGCCAGGCGGGCCTGGCGCGGGCGCCGGGCCCGCAGGGCTGGACCTTGTACCTGCGCGA
CGATGTGGATACCGACGCTTTGCAGGAAGTGTTGCGCGTACACGGCCTGCCGACGCTGGTCGAGCACCTTGACCCGGCCC
AATACGAACTGCGCCTGGGGGCCTTGTACCAGGCCGGCGAATCGGCCACGGCGGCCTTGATTGAGGGCATCGGCGAGCAG
GTCGATCTCGACAGCCTGATCAACGAACTGCCACGTATCGAAGACTTGCTGGAGAGCGATAACGAGGCGCCGGTGATTCG
CCTGATCAATGGCCTGTTCGCCGAAGGCCTGCGCCTGCGCGCCTCGGATATCCATATCGAAACCTTCGAGCAGAGCCTGG
TGGTGCGCCTGCGGGTCGATGGCCATCTGCGGGAGATCCTGCGCCCGCCACGGGCCTTGTCGGCCATGTTGGTGTCGCGG
ATCAAGGTCATGGCGCGCCTGGATATCGCCGAAAAACGCCAGCCCCAGGACGGGCGCATTACCCTGCGCTCCGGGGGGCG
TGAAGTGGATATCCGAGTCTCGACCTTGCCGGGCATCCACGGCGAGCGCGTGGTGATGCGGGTGCTGGATAAACAGGCCA
ATTTGCTGGACCTCAATAACCTGGGCATGCCCGCCGAGGTGGAGCAGGCCCTGCGCGCCTGCCTGTCGCGGCCCAACGGC
ATTTTACTGAGCACAGGGCCCACGGGCTCGGGCAAGACCACCACGCTGTACGCCAGCCTCAATGCGCTGAACGATGGCTC
ATGCAATATCCTGACCGTCGAAGACCCGGTGGAATACGCGATTACCGGCATCGGCCAGACGGCGATCAACCCCCGCGCCG
GCATGACGTTCGCCAGCGGCCTGCGGGCGATCCTGCGCCAGGATCCGGACGTGATCATGCTGGGTGAAATCCGCGACCAG
GAAACCGCACAGATCGCCGTGCAAGCCAGCCTCACCGGGCACCTGGTGCTGTCGACGCTGCACACCAACAGCGCGGTGGG
CGCAGTCACCCGCCTACGTGACATGGGCATCGAACCCTTCCTGATTGCCTCCAGCCTCAAGGGCGTGATGGCCCAGCGCC
TGGTGCGCCGCCTGTGCCATTGCGCAACGCCCGCACCGTTGCAGGCCGCCGAGCGCCAACTGTGGCCGGAACTGGCAACG
CTCGCGCAGAGCTTTCACCCCCAGGGCTGTGACGACTGCCAGGGCAGTGGCTACCACGGGCGGATCGGTCTCTATGAATT
CATCACCCTGGATGCCGGCTTGATCGGCCTGCTGTACGACGGCGCCAGTGAAGCGACGATGCACGAATACCTCGCCGACA
AGCGCCAGAGCCTGACTGCCATGGCCACTGAATGCCTGCGCCGTGGCGACACCAGCCTGGAGCAGGTCCTGCGTGCGGTG
CGCGACTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

41.458

99.585

0.413
  pilB Vibrio campbellii strain DS40M4

47.761

83.402

0.398
  pilB Vibrio cholerae strain A1552

45.913

86.307

0.396
  pilB Vibrio parahaemolyticus RIMD 2210633

45.742

85.27

0.39
  pilB Acinetobacter baumannii D1279779

45.865

82.78

0.38
  pilF Thermus thermophilus HB27

42.019

88.382

0.371
  pilF Neisseria gonorrhoeae MS11

44.724

82.573

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

43.032

84.855

0.365

Multiple sequence alignment