Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACLKM3_RS01160 Genome accession   NZ_CP183897
Coordinates   255917..256975 (+) Length   352 a.a.
NCBI ID   WP_019836139.1    Uniprot ID   -
Organism   Acinetobacter towneri strain M10_NDM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 250917..261975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM3_RS01145 mraY 251124..252242 (+) 1119 WP_019836136.1 phospho-N-acetylmuramoyl-pentapeptide- transferase -
  ACLKM3_RS01150 - 252316..253119 (+) 804 WP_019836137.1 putative RNA methyltransferase -
  ACLKM3_RS01155 ponA 253189..255747 (-) 2559 WP_026437990.1 penicillin-binding protein PBP1a -
  ACLKM3_RS01160 pilM 255917..256975 (+) 1059 WP_019836139.1 pilus assembly protein PilM Machinery gene
  ACLKM3_RS01165 pilN 256975..257610 (+) 636 WP_019836140.1 PilN domain-containing protein Machinery gene
  ACLKM3_RS01170 pilO 257607..258314 (+) 708 WP_019836141.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLKM3_RS01175 pilP 258314..258838 (+) 525 WP_411690781.1 pilus assembly protein PilP Machinery gene
  ACLKM3_RS01180 pilQ 258899..261031 (+) 2133 WP_171066631.1 type IV pilus secretin PilQ Machinery gene
  ACLKM3_RS01185 aroK 261068..261610 (+) 543 WP_026437991.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39009.67 Da        Isoelectric Point: 4.6414

>NTDB_id=1106994 ACLKM3_RS01160 WP_019836139.1 255917..256975(+) (pilM) [Acinetobacter towneri strain M10_NDM]
MRRLYRKPSKGLIGVDISSTSVKLLELSVKNGRYWVESYALVPLPEGSVVEKNILNPEAVGDALERALNLANTQSTSAAF
AIPTSMVITKIIEMDADMTSDEREVQIRMDAEQYIPFPLDEVSLDFEVQPDRLANPNRVNVLLVATRLENVEARVEALEL
GGLKAKIADVESFTIENAFKVFSDTLPMGVNTIGILDIGHSMTTLSVLQNNKIIYTREQTFGGKQLTQEIQNRYGLSYEE
AGRAKKSRTLPDDYDVEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIESDAPSLMVACGLAMRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1106994 ACLKM3_RS01160 WP_019836139.1 255917..256975(+) (pilM) [Acinetobacter towneri strain M10_NDM]
GTGCGTAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGTGTAGATATTAGTTCTACTTCTGTTAAGTTATTAGAACT
CTCTGTTAAGAATGGGAGATATTGGGTCGAAAGCTATGCGCTGGTCCCACTTCCAGAAGGCAGTGTGGTTGAAAAAAACA
TTCTCAATCCAGAAGCAGTCGGGGATGCGCTTGAACGTGCATTGAACTTAGCAAACACGCAATCTACATCGGCAGCATTT
GCAATTCCAACCTCAATGGTGATTACCAAAATCATTGAAATGGATGCAGATATGACCAGCGATGAGCGTGAAGTTCAAAT
TCGTATGGACGCCGAGCAATATATTCCATTCCCATTAGATGAAGTCAGCCTAGACTTTGAAGTCCAACCAGACCGTTTAG
CCAATCCAAACCGCGTCAATGTGTTACTCGTTGCGACACGTTTAGAAAACGTCGAAGCACGAGTAGAAGCATTAGAGTTG
GGTGGTTTGAAAGCCAAAATTGCCGATGTCGAAAGTTTTACCATCGAAAATGCCTTTAAAGTGTTCTCCGATACCCTGCC
AATGGGTGTGAACACTATTGGTATTTTGGACATTGGTCATAGCATGACTACCTTGTCGGTGCTGCAAAATAACAAAATTA
TTTATACCCGCGAACAAACCTTTGGTGGTAAGCAGCTGACACAAGAAATTCAAAACCGTTATGGTTTGTCTTATGAAGAA
GCAGGTCGCGCGAAAAAAAGCCGTACTTTGCCAGATGACTACGATGTAGAAGTGCTCGAACCGTTCTTAGAGGCGGTGGT
GCAACAAGCTGCACGCTCACTGCAGTTTTTCTTTTCATCTTCACAATTTAACGAAATTGATCACATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGTCTTGCGAAGTTGTTGCAGCAAAAACTGGGCTACCGTGTGACCATTGCCAATCCATTCTTG
CAAATGGGCTTCTCTCCACAAATCGACATTAAAAAAATTGAAAGTGATGCACCGTCATTGATGGTGGCATGTGGTTTAGC
AATGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

83.807

100

0.838
  comM Acinetobacter nosocomialis M2

83.807

100

0.838
  comM Acinetobacter baylyi ADP1

78.409

100

0.784
  pilM Legionella pneumophila strain ERS1305867

39.266

100

0.395

Multiple sequence alignment