Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACLKM3_RS01170 Genome accession   NZ_CP183897
Coordinates   257607..258314 (+) Length   235 a.a.
NCBI ID   WP_019836141.1    Uniprot ID   -
Organism   Acinetobacter towneri strain M10_NDM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 252607..263314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM3_RS01155 ponA 253189..255747 (-) 2559 WP_026437990.1 penicillin-binding protein PBP1a -
  ACLKM3_RS01160 pilM 255917..256975 (+) 1059 WP_019836139.1 pilus assembly protein PilM Machinery gene
  ACLKM3_RS01165 pilN 256975..257610 (+) 636 WP_019836140.1 PilN domain-containing protein Machinery gene
  ACLKM3_RS01170 pilO 257607..258314 (+) 708 WP_019836141.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLKM3_RS01175 pilP 258314..258838 (+) 525 WP_411690781.1 pilus assembly protein PilP Machinery gene
  ACLKM3_RS01180 pilQ 258899..261031 (+) 2133 WP_171066631.1 type IV pilus secretin PilQ Machinery gene
  ACLKM3_RS01185 aroK 261068..261610 (+) 543 WP_026437991.1 shikimate kinase AroK -
  ACLKM3_RS01190 aroB 261630..262709 (+) 1080 WP_019836145.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26509.03 Da        Isoelectric Point: 4.5139

>NTDB_id=1106996 ACLKM3_RS01170 WP_019836141.1 257607..258314(+) (pilO) [Acinetobacter towneri strain M10_NDM]
MSLDEFDEVNQDAAVAPKKKMTVEKFFQQFNTLDPNNYGAWPLSVKITCWIFIIFLILALGYFVVIKPKLDAIAAAQAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVTGVNSGLKFKNIRLENEIKQEFFIEQP
ISIEATGDYHAFGSFVSGTAALPRIVTMHDFEIVATENTDKKTDIPQVTYKAQAKTYRYVGSADTQTAGNIGGAE

Nucleotide


Download         Length: 708 bp        

>NTDB_id=1106996 ACLKM3_RS01170 WP_019836141.1 257607..258314(+) (pilO) [Acinetobacter towneri strain M10_NDM]
ATGAGTCTCGATGAATTTGATGAAGTAAATCAGGATGCTGCTGTTGCTCCGAAAAAGAAAATGACCGTTGAAAAGTTTTT
TCAACAGTTCAATACCCTAGATCCAAACAATTACGGTGCTTGGCCGCTTTCGGTCAAAATTACCTGCTGGATTTTTATTA
TTTTCTTGATTTTGGCTTTAGGTTATTTTGTCGTGATCAAGCCAAAGTTAGATGCCATTGCAGCAGCACAAGCACAAGAA
CAAAACTTGTTAAATGAATTCCGCGAAAAAGATTCTAAATTGCGTAATTTGCAGCAATATCAAGCACAATTACAAGAAAT
GGAAGCGAACTTTAATCAACAACTTGAACAATTGCCAAAAGAAACTGAAATTCCAGGATTGGTTGAAGATATTAACGTCA
CTGGCGTGAATTCAGGTTTAAAGTTTAAAAACATTCGTTTAGAGAATGAGATTAAACAAGAGTTCTTTATTGAACAGCCA
ATTTCAATTGAAGCAACAGGTGATTACCATGCCTTTGGTTCATTCGTGAGCGGTACAGCAGCATTGCCACGTATTGTGAC
CATGCACGACTTTGAAATTGTAGCGACTGAAAATACAGACAAGAAAACTGATATTCCGCAAGTAACCTATAAAGCGCAAG
CAAAAACTTATCGTTATGTAGGCAGTGCAGATACACAAACTGCGGGTAATATTGGAGGGGCTGAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

74.667

95.745

0.715
  comO Acinetobacter baylyi ADP1

74.661

94.043

0.702

Multiple sequence alignment