Detailed information NTG1105090

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACNR9X_RS12445
• Genome accession: NZ_CP183293
• Coordinates: 2557522..2558136 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain F68
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2552522..2563136

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNR9X_RS12415 (ACNR9X_12415)	rpsT	2552822..2553088 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACNR9X_RS12420 (ACNR9X_12420)	holA	2553105..2554146 (-)	1042	Protein_2399	DNA polymerase III subunit delta	-
ACNR9X_RS12425 (ACNR9X_12425)	-	2554186..2554340 (-)	155	Protein_2400	hypothetical protein	-
ACNR9X_RS12430 (ACNR9X_12430)	-	2554381..2554515 (+)	135	WP_003152870.1	YqzM family protein	-
ACNR9X_RS12435 (ACNR9X_12435)	comEC	2554534..2556885 (-)	2352	WP_404217255.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACNR9X_RS12440 (ACNR9X_12440)	-	2556886..2557455 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACNR9X_RS12445 (ACNR9X_12445)	comEA	2557522..2558136 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACNR9X_RS12450 (ACNR9X_12450)	comER	2558195..2559016 (+)	822	WP_044053476.1	late competence protein ComER	-
ACNR9X_RS12455 (ACNR9X_12455)	-	2559085..2559822 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
ACNR9X_RS12460 (ACNR9X_12460)	rsfS	2559819..2560175 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACNR9X_RS12465 (ACNR9X_12465)	yqeK	2560172..2560753 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACNR9X_RS12470 (ACNR9X_12470)	-	2560743..2561312 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
ACNR9X_RS12475 (ACNR9X_12475)	yhbY	2561323..2561613 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACNR9X_RS12480 (ACNR9X_12480)	aroE	2561607..2562449 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=1105090 ACNR9X_RS12445 WP_043867297.1 2557522..2558136(-) (comEA) [Bacillus velezensis strain F68]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1105090 ACNR9X_RS12445 WP_043867297.1 2557522..2558136(-) (comEA) [Bacillus velezensis strain F68]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363