Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNOHD_RS16970 Genome accession   NZ_CP183060
Coordinates   3587153..3588856 (-) Length   567 a.a.
NCBI ID   WP_418643306.1    Uniprot ID   -
Organism   Stutzerimonas kunmingensis strain L4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3584128..3594393 3587153..3588856 within 0


Gene organization within MGE regions


Location: 3584128..3594393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNOHD_RS16950 (ACNOHD_16950) yacG 3584128..3584325 (-) 198 WP_312441528.1 DNA gyrase inhibitor YacG -
  ACNOHD_RS16955 (ACNOHD_16955) coaE 3584322..3584930 (-) 609 WP_312441531.1 dephospho-CoA kinase -
  ACNOHD_RS16960 (ACNOHD_16960) pilD 3585062..3585931 (-) 870 WP_278401439.1 prepilin peptidase Machinery gene
  ACNOHD_RS16965 (ACNOHD_16965) pilC 3585934..3587151 (-) 1218 WP_418643305.1 type II secretion system F family protein Machinery gene
  ACNOHD_RS16970 (ACNOHD_16970) pilB 3587153..3588856 (-) 1704 WP_418643306.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNOHD_RS16975 (ACNOHD_16975) - 3589167..3589640 (+) 474 WP_418643307.1 pilin -
  ACNOHD_RS16980 (ACNOHD_16980) - 3590576..3591139 (+) 564 WP_200650223.1 REP-associated tyrosine transposase -
  ACNOHD_RS16985 (ACNOHD_16985) - 3591369..3591809 (+) 441 WP_125862181.1 YtoQ family protein -
  ACNOHD_RS16990 (ACNOHD_16990) cysN 3592103..3594001 (-) 1899 WP_418643308.1 sulfate adenylyltransferase subunit CysN -
  ACNOHD_RS16995 (ACNOHD_16995) - 3594052..3594393 (-) 342 WP_003302815.1 four helix bundle protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61856.86 Da        Isoelectric Point: 5.3486

>NTDB_id=1103911 ACNOHD_RS16970 WP_418643306.1 3587153..3588856(-) (pilB) [Stutzerimonas kunmingensis strain L4]
MSENISLSGLARQMVVAGVLDEKTAQQAQAQAVRGKTPFVTYVVQNKLAKGRAIAELASDQFGVALLDLSALNKESLPKD
LISEKLARQHRVLPLSKRGTKLYVAISDPANHQSIADIQFSTGLTIESILVEDDKLGEAIDKLYESGATGLEELGEVDLD
GVDVETVNEDGKTDEGGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKALYMQALQQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVELPREALLEEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1103911 ACNOHD_RS16970 WP_418643306.1 3587153..3588856(-) (pilB) [Stutzerimonas kunmingensis strain L4]
ATGAGCGAAAACATTTCCCTATCCGGGCTGGCACGGCAGATGGTCGTGGCCGGCGTGTTGGACGAAAAAACGGCCCAGCA
GGCGCAGGCTCAAGCCGTTCGCGGCAAAACACCCTTTGTCACCTATGTAGTGCAGAACAAGTTGGCCAAAGGCCGCGCCA
TAGCCGAGCTTGCTTCCGATCAATTCGGTGTAGCGCTGCTGGACCTGTCTGCGCTGAACAAGGAGTCGCTGCCCAAGGAT
CTGATCAGTGAGAAGTTGGCCCGCCAACATCGGGTGCTGCCGCTTTCCAAGCGCGGCACCAAGCTTTATGTCGCCATCTC
CGATCCGGCTAATCATCAATCGATTGCCGATATCCAGTTCAGTACCGGTTTGACCATCGAGTCGATCCTGGTGGAAGACG
ACAAGCTGGGCGAGGCCATCGATAAGCTGTACGAAAGCGGCGCCACCGGGCTGGAAGAGCTGGGTGAGGTCGATCTGGAT
GGCGTGGATGTCGAGACCGTCAACGAGGATGGAAAAACAGATGAGGGCGGCGAGGCTGCCGATGACGCCCCGGTCGTCCG
CTTCGTCAATAAGATGCTGCTCGATGCCATTCGCGGCGGCTCGTCGGACCTGCACTTCGAACCCTACGAGAAAGCCTATC
GTGTGCGGCTTCGCACGGATGGCATTCTGCATGAAGTCGCTCGCCCACCGATCCAACTGGCGCCCCGCATATCTGCACGC
TTGAAGGTGATGGCCGGGCTGGATATCTCCGAGCGCCGCAAGCCGCAGGACGGGCGGATCAAGATGAAGCTGTCCAAGAC
CAAGGCCATCGACTTCCGGGTGAACACGCTGCCGACGCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGATCCATCCA
GTGCACAGATGGGCATCGATGCGTTGGGGTATGAGGAAGATCAGAAGGCGCTGTATATGCAGGCGCTGCAACAGCCGCAG
GGCATGATCCTGGTGACCGGCCCTACCGGGTCGGGCAAGACGGTCTCCCTTTATACCGGCCTGAACATCCTTAATACGGT
GGATGTGAATATTTCCACAGCGGAAGATCCGGTGGAGATAAACCTCGAGGGTATCAACCAGGTCAACGTCAATCCTCGGC
AGGGCATGGATTTTTCGCAGGCGCTGCGGGCGTTTCTGCGCCAGGACCCGGACATCATCATGGTGGGGGAAATCCGAGAC
CTGGAAACCGCCGAAATTGCCATCAAGGCCGCGCAGACCGGGCATATGGTGATGTCGACGCTGCACACCAACAGCGCAGC
GGAAACCCTGACGCGGCTGCGCAACATGGGCGTCGCGGCATTCAATATCGCGACTTCGGTGAACCTGATAATCGCCCAGC
GGCTGGCGCGCAAGCTGTGCAATAGCTGCAAGAAAGAAGTGGAACTGCCAAGGGAGGCCCTACTGGAAGAAGGCTTCCCT
GCCGATAAGATCGGTACCTTCAAAATCTACGGCCCGGTCGGCTGCGACAATTGCAAGGGTGGATACAAAGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCACTGGCCAGGATTATCATGGAGGACGGCAATTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAACGATCTGCGAACTTCGGCTTTGGTGAAAGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.436

100

0.594
  pilB Acinetobacter baylyi ADP1

59.397

99.471

0.591
  pilB Legionella pneumophila strain ERS1305867

54.912

100

0.552
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.668

99.295

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

52.399

91.887

0.481
  pilB Vibrio cholerae strain A1552

52.437

90.476

0.474
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.45

96.12

0.379

Multiple sequence alignment