Detailed information NTG1103909

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACNOHD_RS16960
• Genome accession: NZ_CP183060
• Coordinates: 3585062..3585931 (-)
• Length: 289 a.a.
• NCBI ID: WP_278401439.1
• Uniprot ID: -
• Organism: Stutzerimonas kunmingensis strain L4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3584128..3594393	3585062..3585931	within	0

Gene organization within MGE regions

Location: 3584128..3594393

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNOHD_RS16950 (ACNOHD_16950)	yacG	3584128..3584325 (-)	198	WP_312441528.1	DNA gyrase inhibitor YacG	-
ACNOHD_RS16955 (ACNOHD_16955)	coaE	3584322..3584930 (-)	609	WP_312441531.1	dephospho-CoA kinase	-
ACNOHD_RS16960 (ACNOHD_16960)	pilD	3585062..3585931 (-)	870	WP_278401439.1	prepilin peptidase	Machinery gene
ACNOHD_RS16965 (ACNOHD_16965)	pilC	3585934..3587151 (-)	1218	WP_418643305.1	type II secretion system F family protein	Machinery gene
ACNOHD_RS16970 (ACNOHD_16970)	pilB	3587153..3588856 (-)	1704	WP_418643306.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACNOHD_RS16975 (ACNOHD_16975)	-	3589167..3589640 (+)	474	WP_418643307.1	pilin	-
ACNOHD_RS16980 (ACNOHD_16980)	-	3590576..3591139 (+)	564	WP_200650223.1	REP-associated tyrosine transposase	-
ACNOHD_RS16985 (ACNOHD_16985)	-	3591369..3591809 (+)	441	WP_125862181.1	YtoQ family protein	-
ACNOHD_RS16990 (ACNOHD_16990)	cysN	3592103..3594001 (-)	1899	WP_418643308.1	sulfate adenylyltransferase subunit CysN	-
ACNOHD_RS16995 (ACNOHD_16995)	-	3594052..3594393 (-)	342	WP_003302815.1	four helix bundle protein	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32349.45 Da
• Isoelectric Point: 7.2758

>NTDB_id=1103909 ACNOHD_RS16960 WP_278401439.1 3585062..3585931(-) (pilD) [Stutzerimonas kunmingensis strain L4]
MMLIDYLASHVLAFVLFAALLGLLIGSFLNVLIYRLPIMMQREWRAQALEYLECPPEPVRERFNLLLPSSRCPHCDHQIR
SWENIPLVSWLALRGKCSSCRAPISSRYPLVELACGLLSGYVAWHFGLTWQAGAMLFLTWGLVAMSMIDIDHQLLPDSLV
LPLLWLGLILNSFGLFVSLESALWGAVAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSVVG
AVLGTILLRVQRAESSTPIPFGPYLAIAGWIALLWGDRITESYLQFARF

Nucleotide

• Length: 870 bp

>NTDB_id=1103909 ACNOHD_RS16960 WP_278401439.1 3585062..3585931(-) (pilD) [Stutzerimonas kunmingensis strain L4]
ATGATGTTGATCGATTATCTGGCCAGCCACGTGCTGGCCTTTGTTTTGTTCGCTGCTTTGCTGGGCTTGTTGATCGGCAG
CTTTCTCAATGTCTTGATTTATCGCCTGCCGATCATGATGCAGCGTGAGTGGCGCGCGCAGGCGCTTGAGTATCTCGAGT
GCCCGCCGGAGCCGGTCCGTGAGCGCTTCAATCTGCTGCTGCCAAGCTCGCGGTGCCCGCACTGTGATCATCAGATTCGC
TCTTGGGAGAATATCCCCTTGGTCAGCTGGCTGGCCTTGCGTGGCAAGTGTTCGTCATGCCGGGCGCCGATCAGCAGTCG
CTATCCCCTTGTGGAGTTGGCGTGCGGCCTGCTTTCTGGCTATGTCGCTTGGCATTTCGGGTTGACCTGGCAGGCTGGCG
CAATGCTGTTCCTGACCTGGGGGTTGGTGGCGATGAGCATGATCGATATCGATCATCAGTTGCTGCCCGATTCGCTGGTT
CTGCCGTTGTTGTGGCTTGGTCTCATCCTGAACAGCTTCGGTTTGTTCGTATCGCTTGAAAGTGCACTGTGGGGAGCGGT
AGCGGGATATCTGAGCCTGTGGTCGGTTTATTGGCTGTTCAAGCTGGTCACCGGCAAAGAAGGTATGGGCTATGGCGATT
TCAAACTGCTTGCGATGTTGGGAGCTTGGGGAGGCTGGCAGGTGTTACCACTTACCATCCTACTGTCGTCGGTGGTTGGG
GCTGTTCTTGGCACAATCCTGTTGCGGGTGCAGCGCGCCGAGAGCAGCACGCCCATCCCTTTTGGTCCCTACTTGGCGAT
CGCCGGCTGGATCGCGTTGCTTTGGGGTGACCGGATTACCGAGAGCTATCTCCAGTTCGCGCGCTTTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	58.484	95.848	0.561
pilD	Vibrio campbellii strain DS40M4	56.044	94.464	0.529
pilD	Acinetobacter baumannii D1279779	52.113	98.27	0.512
pilD	Acinetobacter nosocomialis M2	52.113	98.27	0.512
pilD	Neisseria gonorrhoeae MS11	52.256	92.042	0.481