Detailed information NTG1103800

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACNM7T_RS12455
• Genome accession: NZ_CP183041
• Coordinates: 2562944..2563558 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain Scm
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2557944..2568558

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNM7T_RS12425 (ACNM7T_12425)	rpsT	2558247..2558513 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACNM7T_RS12430 (ACNM7T_12430)	holA	2558530..2559571 (-)	1042	Protein_2401	DNA polymerase III subunit delta	-
ACNM7T_RS12435 (ACNM7T_12435)	-	2559611..2559762 (-)	152	Protein_2402	hypothetical protein	-
ACNM7T_RS12440 (ACNM7T_12440)	-	2559803..2559937 (+)	135	WP_003152870.1	YqzM family protein	-
ACNM7T_RS12445 (ACNM7T_12445)	comEC	2559956..2562307 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACNM7T_RS12450 (ACNM7T_12450)	-	2562308..2562877 (-)	570	WP_094031848.1	ComE operon protein 2	-
ACNM7T_RS12455 (ACNM7T_12455)	comEA	2562944..2563558 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACNM7T_RS12460 (ACNM7T_12460)	comER	2563617..2564438 (+)	822	WP_012118027.1	late competence protein ComER	-
ACNM7T_RS12465 (ACNM7T_12465)	-	2564507..2565244 (-)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
ACNM7T_RS12470 (ACNM7T_12470)	rsfS	2565241..2565597 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACNM7T_RS12475 (ACNM7T_12475)	yqeK	2565615..2566175 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACNM7T_RS12480 (ACNM7T_12480)	-	2566165..2566734 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACNM7T_RS12485 (ACNM7T_12485)	yhbY	2566745..2567035 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ACNM7T_RS12490 (ACNM7T_12490)	aroE	2567029..2567871 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=1103800 ACNM7T_RS12455 WP_043021637.1 2562944..2563558(-) (comEA) [Bacillus amyloliquefaciens strain Scm]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1103800 ACNM7T_RS12455 WP_043021637.1 2562944..2563558(-) (comEA) [Bacillus amyloliquefaciens strain Scm]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363