Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNR0F_RS03485 Genome accession   NZ_CP183037
Coordinates   657468..658652 (-) Length   394 a.a.
NCBI ID   WP_051291979.1    Uniprot ID   -
Organism   Kingella kingae strain URMC_2412A945     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 652468..663652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNR0F_RS03445 (ACNR0F_03445) tatC 653407..654171 (-) 765 WP_003786159.1 twin-arginine translocase subunit TatC -
  ACNR0F_RS03450 (ACNR0F_03450) tatB 654168..654674 (-) 507 WP_038310885.1 Sec-independent protein translocase protein TatB -
  ACNR0F_RS03455 (ACNR0F_03455) tatA 654676..654879 (-) 204 WP_003786162.1 Sec-independent protein translocase subunit TatA -
  ACNR0F_RS03460 (ACNR0F_03460) - 654931..655251 (-) 321 WP_003790149.1 histidine triad nucleotide-binding protein -
  ACNR0F_RS03465 (ACNR0F_03465) - 655323..655652 (-) 330 WP_418605078.1 phosphoribosyl-ATP diphosphatase -
  ACNR0F_RS03470 (ACNR0F_03470) hisI 655680..656075 (-) 396 WP_003790152.1 phosphoribosyl-AMP cyclohydrolase -
  ACNR0F_RS03475 (ACNR0F_03475) - 656085..656615 (-) 531 WP_003790154.1 CreA family protein -
  ACNR0F_RS03480 (ACNR0F_03480) - 656630..657385 (-) 756 WP_003790157.1 sulfite exporter TauE/SafE family protein -
  ACNR0F_RS03485 (ACNR0F_03485) pilU 657468..658652 (-) 1185 WP_051291979.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNR0F_RS03490 (ACNR0F_03490) - 658834..659316 (+) 483 WP_003786169.1 Lrp/AsnC family transcriptional regulator -
  ACNR0F_RS03495 (ACNR0F_03495) - 659422..660762 (+) 1341 WP_003790160.1 DUF3482 domain-containing protein -
  ACNR0F_RS03500 (ACNR0F_03500) - 660925..661770 (+) 846 WP_003790161.1 PHP domain-containing protein -
  ACNR0F_RS03505 (ACNR0F_03505) - 661898..662053 (-) 156 WP_418605151.1 hypothetical protein -
  ACNR0F_RS03510 (ACNR0F_03510) - 662016..662186 (+) 171 Protein_691 sodium:proton antiporter -
  ACNR0F_RS03515 (ACNR0F_03515) - 662278..663344 (+) 1067 Protein_692 sodium:proton antiporter -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 43700.17 Da        Isoelectric Point: 6.0102

>NTDB_id=1103709 ACNR0F_RS03485 WP_051291979.1 657468..658652(-) (pilU) [Kingella kingae strain URMC_2412A945]
MQVIIPPINEEMRKFIHGLLTHMVNNKGSDLFITAGYPPAIKLDGKLTKITDKPLTADHAMQIARSIMDDKQAEEFLTTN
ECNFAISLSGVSRFRINAMVQRGAAALVCRVITSDIPKFDNMNLPPVLKQVVMEKRGLVIFVGGTGSGKSTSLAAMIDYR
NENSYGHIITIEDPIEFVHPHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMAT
LHANNSNQALDRIINFFPEERRTQLLNDLSLNLKGFISQRLVPKKQGKGRVAAVEILLNSPLISELVLHGDIHAVKEIMA
KSRDIGMQTFDQSLFDLYEADLISYDDALRNADSVNDLRLQIQLNSKKNATAGSGGVLDGLSLTDYAPPEAEEK

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=1103709 ACNR0F_RS03485 WP_051291979.1 657468..658652(-) (pilU) [Kingella kingae strain URMC_2412A945]
ATGCAAGTTATCATTCCACCCATCAACGAAGAAATGCGTAAATTTATCCATGGCTTGCTCACGCACATGGTCAATAACAA
AGGCTCGGATTTATTTATCACGGCTGGCTATCCACCTGCGATAAAATTGGACGGCAAATTAACCAAAATCACCGACAAGC
CTTTAACCGCCGACCACGCCATGCAAATTGCGCGTTCGATTATGGACGACAAGCAAGCCGAAGAATTTTTAACCACCAAC
GAATGCAATTTTGCGATTAGCTTGTCAGGCGTGTCGCGCTTTCGTATCAACGCCATGGTGCAACGTGGTGCAGCAGCTTT
GGTTTGCCGTGTGATTACCAGCGACATTCCCAAATTCGACAACATGAACTTACCGCCTGTGTTAAAACAAGTCGTGATGG
AAAAACGTGGTTTGGTGATTTTTGTCGGCGGTACAGGTTCGGGTAAATCCACATCGTTGGCGGCGATGATTGATTACCGC
AACGAAAATAGCTACGGTCATATCATCACGATTGAAGACCCGATTGAATTTGTTCACCCACACAAAAACTGCATCATCAC
GCAACGCGAAGTGGGTGTGGACACCGAAAACTGGTTCGCCGCGCTCAAAAACACACTGCGCCAAGCACCCGATGTGATTT
TGATTGGTGAGATTCGTGACCGCGAAACGATGGACTACGCACTTGCCTTTGCCGAAACAGGGCACTTGTGTATGGCAACA
CTGCACGCGAATAACTCCAACCAGGCCCTTGACCGCATTATTAACTTCTTCCCCGAAGAACGCCGTACCCAGTTGCTGAA
CGACTTGTCGCTCAACTTAAAAGGCTTTATTTCGCAACGCCTTGTCCCCAAAAAACAAGGCAAAGGACGTGTGGCTGCGG
TAGAAATCCTGCTCAATTCGCCGTTGATTTCCGAATTGGTGTTGCATGGCGACATTCATGCGGTAAAAGAAATTATGGCA
AAATCGCGCGATATTGGTATGCAAACGTTCGACCAATCGCTGTTTGATTTGTATGAAGCCGATTTGATTTCGTATGACGA
TGCCTTGCGTAATGCTGACTCGGTAAACGATTTGCGCCTGCAAATTCAGTTAAACAGCAAGAAAAACGCAACGGCGGGTT
CGGGTGGCGTGCTGGACGGCTTGTCTTTGACGGATTACGCACCGCCAGAAGCCGAAGAAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(91-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.239

89.34

0.52
  pilU Acinetobacter baylyi ADP1

58.239

89.34

0.52
  pilU Vibrio cholerae strain A1552

53.406

93.147

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.162

93.909

0.396
  pilT Legionella pneumophila strain Lp02

43.844

84.518

0.371
  pilT Legionella pneumophila strain ERS1305867

43.844

84.518

0.371

Multiple sequence alignment