Detailed information NTG1102288

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACNFKI_RS14015
• Genome accession: NZ_CP182571
• Coordinates: 2797434..2798048 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain DY201
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2792434..2803048

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNFKI_RS13985 (ACNFKI_13985)	rpsT	2792734..2793000 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACNFKI_RS13990 (ACNFKI_13990)	holA	2793017..2794058 (-)	1042	Protein_2713	DNA polymerase III subunit delta	-
ACNFKI_RS13995 (ACNFKI_13995)	-	2794098..2794252 (-)	155	Protein_2714	hypothetical protein	-
ACNFKI_RS14000 (ACNFKI_14000)	-	2794293..2794427 (+)	135	WP_003152870.1	YqzM family protein	-
ACNFKI_RS14005 (ACNFKI_14005)	comEC	2794446..2796797 (-)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACNFKI_RS14010 (ACNFKI_14010)	-	2796798..2797367 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACNFKI_RS14015 (ACNFKI_14015)	comEA	2797434..2798048 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACNFKI_RS14020 (ACNFKI_14020)	comER	2798107..2798928 (+)	822	WP_014305449.1	late competence protein ComER	-
ACNFKI_RS14025 (ACNFKI_14025)	-	2798997..2799734 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
ACNFKI_RS14030 (ACNFKI_14030)	rsfS	2799731..2800087 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACNFKI_RS14035 (ACNFKI_14035)	yqeK	2800084..2800665 (-)	582	WP_057080474.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACNFKI_RS14040 (ACNFKI_14040)	-	2800655..2801224 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
ACNFKI_RS14045 (ACNFKI_14045)	yhbY	2801235..2801525 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACNFKI_RS14050 (ACNFKI_14050)	aroE	2801519..2802361 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=1102288 ACNFKI_RS14015 WP_003152867.1 2797434..2798048(-) (comEA) [Bacillus velezensis strain DY201]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1102288 ACNFKI_RS14015 WP_003152867.1 2797434..2798048(-) (comEA) [Bacillus velezensis strain DY201]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363