Detailed information NTG1101137

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACNVPQ_RS12460
• Genome accession: NZ_CP182318
• Coordinates: 2562900..2563514 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain Scm3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2557900..2568514

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNVPQ_RS12430	rpsT	2558203..2558469 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACNVPQ_RS12435	holA	2558486..2559527 (-)	1042	Protein_2402	DNA polymerase III subunit delta	-
ACNVPQ_RS12440	-	2559567..2559718 (-)	152	Protein_2403	hypothetical protein	-
ACNVPQ_RS12445	-	2559759..2559893 (+)	135	WP_003152870.1	YqzM family protein	-
ACNVPQ_RS12450	comEC	2559912..2562263 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACNVPQ_RS12455	-	2562264..2562833 (-)	570	WP_094031848.1	ComE operon protein 2	-
ACNVPQ_RS12460	comEA	2562900..2563514 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACNVPQ_RS12465	comER	2563573..2564394 (+)	822	WP_012118027.1	late competence protein ComER	-
ACNVPQ_RS12470	-	2564463..2565200 (-)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
ACNVPQ_RS12475	rsfS	2565197..2565553 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACNVPQ_RS12480	yqeK	2565571..2566131 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACNVPQ_RS12485	-	2566121..2566690 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACNVPQ_RS12490	yhbY	2566701..2566991 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ACNVPQ_RS12495	aroE	2566985..2567827 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=1101137 ACNVPQ_RS12460 WP_043021637.1 2562900..2563514(-) (comEA) [Bacillus velezensis strain Scm3]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1101137 ACNVPQ_RS12460 WP_043021637.1 2562900..2563514(-) (comEA) [Bacillus velezensis strain Scm3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363