Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACGHTT_RS03910 Genome accession   NZ_AP031497
Coordinates   889481..890680 (+) Length   399 a.a.
NCBI ID   WP_390593643.1    Uniprot ID   -
Organism   Simiduia litorea strain KMM 9504     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 884481..895680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHTT_RS03890 (R50072_07670) - 885168..886430 (+) 1263 WP_390593637.1 hypothetical protein -
  ACGHTT_RS03895 (R50072_07680) - 886498..887373 (+) 876 WP_390593639.1 hypothetical protein -
  ACGHTT_RS03900 (R50072_07690) - 887411..888556 (-) 1146 WP_390593641.1 hypothetical protein -
  ACGHTT_RS03905 (R50072_07700) - 888638..889354 (-) 717 WP_390593642.1 tRNA (adenine(22)-N(1))-methyltransferase TrmK -
  ACGHTT_RS03910 (R50072_07710) pilU 889481..890680 (+) 1200 WP_390593643.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHTT_RS03915 (R50072_07720) - 890815..891600 (+) 786 WP_390593645.1 substrate-binding periplasmic protein -
  ACGHTT_RS03920 (R50072_07730) - 891671..892132 (+) 462 WP_390593646.1 nuclear transport factor 2 family protein -
  ACGHTT_RS03925 (R50072_07740) - 892439..894718 (+) 2280 WP_390593648.1 NADP-dependent malic enzyme -
  ACGHTT_RS03930 (R50072_07750) - 894738..894908 (+) 171 WP_390593649.1 hypothetical protein -
  ACGHTT_RS03935 (R50072_07760) - 894913..895575 (+) 663 WP_390593650.1 glutathione S-transferase -

Sequence

Protein


Download         Length: 399 a.a.        Molecular weight: 44248.49 Da        Isoelectric Point: 6.2967

>NTDB_id=109893 ACGHTT_RS03910 WP_390593643.1 889481..890680(+) (pilU) [Simiduia litorea strain KMM 9504]
MSTPIDNLLNILAKKDGSDLYLSTGAPPCAKFQGHLKPLNQTPYTAGEIEAIADAIMDDEQRKVFSAELEMNLAISIAGV
GRFRINIFKQRNEVSIVARNIKADIPKFEDLRLPEVLKDIILTKRGLVLFVGATGSGKSTSLAALIDHRNSTKAGHIITI
EDPVEYVHRHKKSIINQREVGVDTRSFRNALKNTLRQAPDVILIGEIRDRETMEHALEFAETGHLAISTLHANNANQALE
RIINFFPEERRPQLLLSLSQNLRAFVSQRLIPTIDGKRCAAVEILIGTKTIQEMVYTSRFHEIKEIMEKSENLGMQTFDG
ALFHLFKEGRISQDEAIANADSANNLRLRIKLDSRKVGAEAQPSADAAPAEVEKPAGLSFGSLSLEEMDGDDEENGIHK

Nucleotide


Download         Length: 1200 bp        

>NTDB_id=109893 ACGHTT_RS03910 WP_390593643.1 889481..890680(+) (pilU) [Simiduia litorea strain KMM 9504]
ATGAGCACGCCAATAGATAATTTACTCAACATACTTGCGAAGAAAGATGGATCAGATTTGTATCTGAGCACCGGTGCACC
ACCTTGCGCTAAATTCCAGGGGCATCTAAAGCCGCTCAATCAAACCCCTTATACCGCCGGTGAAATTGAAGCAATTGCCG
ACGCCATTATGGATGATGAGCAGCGCAAAGTTTTTAGTGCGGAGCTAGAAATGAATTTGGCGATATCCATTGCTGGTGTT
GGCAGGTTTCGCATCAACATTTTTAAACAGCGCAATGAAGTGTCTATCGTGGCGCGCAATATCAAGGCTGACATTCCCAA
GTTTGAGGATCTTCGATTACCCGAGGTGTTAAAAGACATAATTCTCACTAAGCGCGGCTTGGTGCTTTTTGTTGGCGCCA
CGGGTTCGGGTAAGTCGACCTCGCTAGCGGCGCTCATTGATCACCGCAACAGTACGAAGGCTGGGCACATTATTACTATC
GAAGACCCAGTGGAGTATGTGCATCGGCATAAGAAGTCGATTATTAATCAGCGCGAGGTGGGGGTTGATACCCGCAGCTT
TCGCAACGCCTTGAAAAACACCTTGCGCCAGGCCCCGGATGTGATTCTTATTGGCGAAATTCGGGATCGAGAAACCATGG
AGCATGCGCTTGAGTTTGCCGAAACCGGGCACCTGGCCATCTCCACGTTACATGCCAATAACGCCAATCAGGCGCTGGAG
CGGATTATCAACTTCTTTCCGGAAGAACGACGACCACAGCTGTTGTTGTCGCTATCGCAGAACTTGCGTGCCTTCGTATC
CCAGCGCCTTATCCCCACCATTGACGGCAAGCGCTGTGCTGCGGTGGAAATTCTCATTGGCACCAAGACAATTCAGGAGA
TGGTCTACACCAGTCGCTTTCATGAAATTAAGGAAATTATGGAGAAATCGGAGAATTTGGGGATGCAAACCTTCGATGGC
GCGTTATTCCACCTATTTAAGGAAGGGCGTATTAGCCAAGATGAGGCAATCGCCAATGCCGACTCTGCCAACAACTTGCG
CCTGCGGATAAAGCTCGATAGCCGCAAGGTCGGTGCTGAGGCACAACCTAGCGCCGATGCGGCACCGGCAGAGGTTGAGA
AGCCGGCCGGCCTGTCATTTGGCAGCTTGTCATTGGAAGAAATGGATGGTGACGATGAAGAGAATGGCATCCATAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.366

88.972

0.484
  pilU Acinetobacter baylyi ADP1

52.286

87.719

0.459
  pilU Vibrio cholerae strain A1552

48.276

87.218

0.421
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.107

93.734

0.376
  pilT Pseudomonas stutzeri DSM 10701

42.105

85.714

0.361
  pilT Pseudomonas aeruginosa PAK

42.105

85.714

0.361

Multiple sequence alignment