Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACM26W_RS11205 Genome accession   NZ_CP181044
Coordinates   2326256..2328052 (+) Length   598 a.a.
NCBI ID   WP_416137080.1    Uniprot ID   -
Organism   Halomonas sp. HK25     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2321256..2333052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM26W_RS11150 (ACM26W_11150) - 2321359..2322504 (-) 1146 WP_416137072.1 RNA-guided endonuclease InsQ/TnpB family protein -
  ACM26W_RS11155 (ACM26W_11155) - 2322678..2322977 (+) 300 WP_416137073.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ACM26W_RS11160 (ACM26W_11160) - 2322974..2323270 (+) 297 WP_416137074.1 addiction module antidote protein -
  ACM26W_RS11165 (ACM26W_11165) - 2323293..2323658 (-) 366 WP_416137075.1 helix-turn-helix domain-containing protein -
  ACM26W_RS11170 (ACM26W_11170) - 2323663..2323995 (-) 333 WP_416137076.1 type II toxin-antitoxin system HigB family toxin -
  ACM26W_RS11175 (ACM26W_11175) - 2324177..2324416 (+) 240 WP_416137077.1 antitoxin MazE family protein -
  ACM26W_RS11180 (ACM26W_11180) - 2324413..2324736 (+) 324 WP_416137078.1 type II toxin-antitoxin system PemK/MazF family toxin -
  ACM26W_RS11185 (ACM26W_11185) - 2324765..2325028 (-) 264 WP_416140865.1 DUF2442 domain-containing protein -
  ACM26W_RS11190 (ACM26W_11190) - 2325009..2325122 (-) 114 WP_416140866.1 DUF4160 domain-containing protein -
  ACM26W_RS11195 (ACM26W_11195) - 2325173..2325256 (-) 84 Protein_2193 DUF4160 domain-containing protein -
  ACM26W_RS11200 (ACM26W_11200) - 2325429..2325881 (-) 453 WP_416137079.1 pilin -
  ACM26W_RS11205 (ACM26W_11205) pilB 2326256..2328052 (+) 1797 WP_416137080.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM26W_RS11210 (ACM26W_11210) pilC 2328089..2329321 (+) 1233 WP_416137081.1 type II secretion system F family protein Machinery gene
  ACM26W_RS11215 (ACM26W_11215) pilD 2329430..2330281 (+) 852 WP_416137082.1 prepilin peptidase Machinery gene

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 65211.92 Da        Isoelectric Point: 6.3696

>NTDB_id=1097887 ACM26W_RS11205 WP_416137080.1 2326256..2328052(+) (pilB) [Halomonas sp. HK25]
MNDYRTPPSAPPLNSPHHAALEGADGAAGLRGLARRLVVDGRINHAAAVRAEQEAREGEVSLMEHVIDTGLVSARQATLS
AAWEYGLPMLDLDAIRPETLPRADAFPEKILRKLKVLPLVKRGHRLTVAVPYPSTLALLDELQFATGLTPEGVLAPVDQL
GPVLESYLASTEKSMLDELKGVDDAIGELGYAGDEEASGEVDVQAAANAASEDAPIIKFVNKILLDAIHRGASDIHFEPY
ETSYRVRFRIDGMLHDVAHPPFAMRTRIAARLKVMSRLDISERRLPQDGAIKLRLSKTRSIDFRVNSLPTVFGEKLVLRI
LDPASAQLGIEQLGFTARQREMYETVLDQPQGMILVTGPTGSGKTVTLYTGINLLNEVERNICTAEDPVEIKVPGVNQVN
VLPKIGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGLAPFNIASSVSL
IIAQRLARKLCKHCKQPADLPHEVLAAQGFSEEEISQATFFEPVGCKHCTKGFKGRVGIYEVVPISHAMSQMIMKNGNSM
EFHDLARQEGHPDLRRSGLMKAMQGMTSLAEINRVTKD

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=1097887 ACM26W_RS11205 WP_416137080.1 2326256..2328052(+) (pilB) [Halomonas sp. HK25]
ATGAACGACTACCGCACCCCGCCGAGCGCGCCCCCCCTGAACTCACCGCACCATGCCGCGCTGGAGGGCGCCGACGGCGC
GGCGGGGCTGCGCGGCCTGGCGCGGCGCCTGGTGGTGGATGGGCGCATCAACCACGCCGCCGCGGTGCGCGCCGAGCAGG
AGGCCCGGGAGGGCGAGGTGTCGCTGATGGAGCACGTCATCGACACCGGGCTGGTCTCGGCCCGCCAGGCCACGCTGTCG
GCGGCCTGGGAGTATGGCCTGCCGATGCTGGACCTGGACGCCATCCGCCCGGAGACGCTGCCCCGGGCCGATGCCTTCCC
CGAGAAGATCCTGCGCAAGCTCAAGGTGCTGCCGCTGGTCAAGCGCGGCCACCGGCTCACCGTGGCGGTGCCCTACCCCT
CCACCCTGGCGCTGCTCGATGAGCTGCAGTTCGCCACCGGCCTGACCCCGGAGGGGGTGCTGGCCCCGGTGGACCAGCTC
GGCCCGGTGCTGGAGAGCTATCTGGCCTCCACCGAGAAGAGCATGCTCGACGAGCTCAAGGGGGTGGACGACGCCATCGG
CGAGCTGGGCTACGCCGGCGACGAGGAGGCCAGCGGCGAGGTGGATGTGCAGGCGGCCGCCAACGCCGCCAGCGAAGACG
CGCCGATCATCAAGTTCGTCAACAAGATCCTGCTCGATGCCATCCACCGCGGCGCCTCGGACATCCACTTCGAGCCCTAC
GAGACCAGCTACCGGGTGCGCTTTCGCATCGACGGCATGCTCCACGACGTGGCCCACCCGCCCTTCGCCATGCGCACGCG
CATCGCCGCGCGCCTCAAGGTGATGTCGCGCCTGGATATCTCCGAGCGGCGCCTGCCCCAGGATGGCGCCATCAAGCTCA
GGCTCTCCAAGACCCGCTCCATCGACTTTCGCGTCAACTCGCTGCCCACGGTGTTCGGCGAGAAACTGGTGCTGCGGATC
CTCGACCCCGCCTCGGCGCAGCTGGGCATCGAGCAGCTCGGCTTCACGGCCAGGCAGCGGGAGATGTACGAGACGGTGCT
CGACCAGCCCCAGGGCATGATACTCGTCACCGGCCCCACCGGCAGCGGCAAGACGGTGACCCTCTACACCGGCATCAACC
TGCTCAACGAGGTGGAGCGCAACATCTGCACCGCCGAGGACCCGGTGGAGATCAAGGTGCCCGGGGTCAACCAGGTCAAC
GTGCTGCCCAAGATCGGCCTGGACTTCGCCAGTGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGGTGATGGTCGG
CGAGATCCGCGACCTGGAGACCGCCGAGATCGCGGTGAAGGCCTCCCAGACCGGCCACCTGGTGCTCTCCACGGTGCACA
CCAACTCCGCCGCCGAGACCCTGACCCGCCTGGCCAACATGGGCCTGGCGCCCTTCAATATCGCCAGTTCGGTGAGCCTG
ATCATCGCCCAGCGCCTGGCTCGCAAGCTGTGCAAGCACTGCAAGCAGCCGGCGGATCTCCCCCACGAGGTGCTGGCCGC
CCAGGGCTTCTCCGAGGAGGAGATCAGCCAGGCCACCTTCTTCGAGCCGGTGGGCTGCAAGCACTGCACCAAGGGCTTCA
AGGGCCGGGTGGGCATCTACGAGGTGGTGCCGATCAGCCACGCCATGAGCCAGATGATCATGAAGAACGGCAACTCCATG
GAGTTCCACGACCTGGCCCGCCAGGAAGGCCACCCGGACCTGCGCCGCAGCGGCCTGATGAAGGCCATGCAGGGGATGAC
CAGCCTGGCCGAGATCAATCGGGTAACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(222-489)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

51.389

96.321

0.495
  pilB Acinetobacter baumannii D1279779

50.865

96.656

0.492
  pilB Legionella pneumophila strain ERS1305867

51.404

95.318

0.49
  pilB Vibrio cholerae strain A1552

49.351

90.134

0.445
  pilB Vibrio campbellii strain DS40M4

48.188

92.308

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

47.55

92.14

0.438
  pilF Neisseria gonorrhoeae MS11

44.834

95.485

0.428
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.201

92.14

0.361

Multiple sequence alignment