Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACM26W_RS11215 Genome accession   NZ_CP181044
Coordinates   2329430..2330281 (+) Length   283 a.a.
NCBI ID   WP_416137082.1    Uniprot ID   -
Organism   Halomonas sp. HK25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2324430..2335281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM26W_RS11185 (ACM26W_11185) - 2324765..2325028 (-) 264 WP_416140865.1 DUF2442 domain-containing protein -
  ACM26W_RS11190 (ACM26W_11190) - 2325009..2325122 (-) 114 WP_416140866.1 DUF4160 domain-containing protein -
  ACM26W_RS11195 (ACM26W_11195) - 2325173..2325256 (-) 84 Protein_2193 DUF4160 domain-containing protein -
  ACM26W_RS11200 (ACM26W_11200) - 2325429..2325881 (-) 453 WP_416137079.1 pilin -
  ACM26W_RS11205 (ACM26W_11205) pilB 2326256..2328052 (+) 1797 WP_416137080.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM26W_RS11210 (ACM26W_11210) pilC 2328089..2329321 (+) 1233 WP_416137081.1 type II secretion system F family protein Machinery gene
  ACM26W_RS11215 (ACM26W_11215) pilD 2329430..2330281 (+) 852 WP_416137082.1 prepilin peptidase Machinery gene
  ACM26W_RS11220 (ACM26W_11220) - 2332877..2333455 (-) 579 WP_416137083.1 hypoxanthine-guanine phosphoribosyltransferase -
  ACM26W_RS11225 (ACM26W_11225) nagZ 2333502..2334512 (-) 1011 WP_416137084.1 beta-N-acetylhexosaminidase -
  ACM26W_RS11230 (ACM26W_11230) - 2334608..2335270 (-) 663 WP_416137085.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 30547.87 Da        Isoelectric Point: 8.0033

>NTDB_id=1097889 ACM26W_RS11215 WP_416137082.1 2329430..2330281(+) (pilD) [Halomonas sp. HK25]
MLDLPPPLLWPLAAFIGLSLGSFLNVVIVRLPVMLMLGWRAEAREALELPDEAQPRFNLATPRSMCPSCETPIAWHDNLP
VVGWLKRRGRCASCGTGISPQYPLVELAGGALTLAVVALFGPSWQGLFVVGACLALLAMAVIDLRTQLLPDLLTLPLLWA
GLLYQLLFQPLLLPSAVIGAMAGYLVLWSFYWAFKLITGKEGMGYGDFKLLAALGAWMGWQTLPLLLILSAGIGAIIGIL
VQLAMPRLRGAPMPFGPYLALAGWVALLAGEPLMAAYGRFFLL

Nucleotide


Download         Length: 852 bp        

>NTDB_id=1097889 ACM26W_RS11215 WP_416137082.1 2329430..2330281(+) (pilD) [Halomonas sp. HK25]
TTGCTTGACCTCCCCCCTCCCCTGCTCTGGCCGCTGGCCGCCTTTATCGGCCTGTCCCTGGGCAGCTTTCTCAACGTGGT
GATCGTGCGCCTGCCGGTGATGCTGATGCTGGGCTGGCGGGCCGAGGCCCGTGAGGCCCTGGAGCTGCCCGACGAGGCGC
AGCCCCGCTTCAACCTGGCCACGCCGCGCTCGATGTGCCCGAGCTGCGAGACGCCCATCGCCTGGCACGACAACCTGCCG
GTGGTCGGCTGGCTCAAGCGCCGCGGGCGCTGCGCCAGCTGTGGAACAGGCATCAGCCCCCAGTACCCGCTGGTGGAGCT
GGCCGGTGGCGCGCTGACGCTCGCCGTGGTGGCGCTGTTCGGGCCGAGCTGGCAGGGGCTCTTCGTGGTGGGGGCCTGCC
TGGCGCTGCTGGCCATGGCGGTGATCGACCTGCGCACCCAGCTCTTGCCGGACCTGCTCACCCTGCCGCTGCTGTGGGCG
GGGCTGCTCTACCAGCTGTTGTTCCAGCCGCTGCTGCTACCGAGCGCGGTGATCGGTGCCATGGCGGGGTATCTGGTGCT
GTGGAGCTTCTACTGGGCGTTCAAGCTGATCACCGGCAAGGAGGGCATGGGCTACGGCGACTTCAAGCTGCTAGCCGCGC
TCGGCGCCTGGATGGGCTGGCAAACCCTGCCGCTGCTGCTGATTCTCTCGGCGGGGATCGGCGCCATCATCGGCATCCTG
GTGCAGCTGGCGATGCCGCGGCTGCGCGGCGCGCCGATGCCCTTCGGGCCCTACCTGGCGCTGGCCGGCTGGGTGGCGCT
GCTCGCCGGGGAGCCACTGATGGCGGCGTATGGGCGGTTCTTTCTGCTGTAG

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(133-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(16-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

51.418

99.647

0.512
  pilD Vibrio campbellii strain DS40M4

48.571

98.94

0.481
  pilD Acinetobacter baumannii D1279779

47.81

96.82

0.463
  pilD Acinetobacter nosocomialis M2

47.08

96.82

0.456
  pilD Neisseria gonorrhoeae MS11

45.848

97.88

0.449

Multiple sequence alignment