Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACM2ZB_RS19780 Genome accession   NZ_CP180589
Coordinates   4294531..4296258 (+) Length   575 a.a.
NCBI ID   WP_416056688.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain ZSL2493     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4289531..4301258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM2ZB_RS19760 (ACM2ZB_19760) coaE 4290910..4291521 (-) 612 WP_416056686.1 dephospho-CoA kinase -
  ACM2ZB_RS19765 (ACM2ZB_19765) - 4291532..4292395 (-) 864 WP_313441785.1 A24 family peptidase -
  ACM2ZB_RS19770 (ACM2ZB_19770) pilC 4292405..4293664 (-) 1260 WP_416058588.1 type II secretion system F family protein Machinery gene
  ACM2ZB_RS19775 (ACM2ZB_19775) - 4294017..4294412 (+) 396 WP_416056687.1 pilin -
  ACM2ZB_RS19780 (ACM2ZB_19780) pilB 4294531..4296258 (+) 1728 WP_416056688.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM2ZB_RS19785 (ACM2ZB_19785) pilR 4296473..4297846 (-) 1374 WP_416056689.1 sigma-54-dependent transcriptional regulator Regulator
  ACM2ZB_RS19790 (ACM2ZB_19790) - 4297901..4299514 (-) 1614 WP_313441775.1 sensor histidine kinase -
  ACM2ZB_RS19795 (ACM2ZB_19795) sucC 4299982..4301151 (+) 1170 WP_108766110.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62159.14 Da        Isoelectric Point: 6.2491

>NTDB_id=1096864 ACM2ZB_RS19780 WP_416056688.1 4294531..4296258(+) (pilB) [Stenotrophomonas maltophilia strain ZSL2493]
MNAVTTANLVGITGLARRLVQDGALDEAGARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHTSHDSLGDSLGGD
DDALANLDISGGDEDMAGGDSGIDAKGDDTPVVKFVNKVLIDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSHCKRPTTLPDHA
LLAEGFTQAQLDAGIQLHEAVGCDECTEGYKGRTGLYQVMPMTDEISTIVLAGGNALQIAEAAQAIGVNDLRQSALKKVA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1096864 ACM2ZB_RS19780 WP_416056688.1 4294531..4296258(+) (pilB) [Stenotrophomonas maltophilia strain ZSL2493]
ATGAACGCAGTCACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGCGCCCTGGATGA
AGCGGGCGCCCGTGATGCCATGGCCAAGGCGGCGGCGGCCAGGCAACCGCTGCCCACCTGGTTCGCACAGAACAAAGTCG
TGGGGGCCGCTCAGCTGGCGGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGATGTATCCACCTTCGATGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGTGGGAAGCT
GTTCGTCGGCACCAGCAATCCGACCCACTCGCTGGACGAGATCAAGTTCCATACCAACCTGGTGGTGGAGCCGATCCTGG
TCGACGAGGACCAGATCCGCCGCACGCTGGAACAGTGGCACACCAGTCACGACAGCCTGGGCGATTCGCTGGGCGGTGAC
GACGATGCGCTGGCCAACCTGGATATTTCCGGTGGCGACGAGGACATGGCCGGCGGTGACAGCGGCATCGACGCCAAGGG
CGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGATCGATGCGATCCGCAAGGGCGCCTCGGACATCCACTTCG
AGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTCGCGCGCGCGCCGGTGAAGTTG
AACCAGCGCATCGCCGCGCGTCTGAAGGTGATGGCGCAGCTGGATATCGCCGAAAAGCGCGTGCCGCAGGACGGGCGCAT
CAAGCTGAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGTACCCTGCCCACGCTGTTCGGCGAAAAGGTGGTGC
TGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAACCGGACCAGCAGAAGCTGTTCCTG
GAAGCCATCCACAAGCCTTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCCGCCCTGCGCTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTCGGTGAAATCCGCGACCTGGAGACGGCGGAAATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTTTCAAC
CCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCACCGTACAACATCACCAGCTCGG
TCACCCTGGTGATCGCCCAGCGCCTGGCGCGCCGTCTGTGCAGCCACTGCAAGCGGCCGACGACGCTTCCGGATCACGCA
CTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCCAGCTGCATGAGGCGGTCGGCTGCGACGAATGCAC
CGAGGGCTACAAGGGCCGTACCGGCCTGTACCAGGTGATGCCGATGACCGATGAGATTTCCACCATCGTGCTGGCCGGCG
GCAACGCGCTGCAGATTGCCGAGGCTGCGCAGGCGATCGGAGTGAACGATCTGCGCCAGTCGGCATTGAAGAAGGTCGCA
GCGGGCGTCACCAGCCTGGCCGAGATCAACCGCGTCACTAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-146)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.882

100

0.562
  pilB Acinetobacter baumannii D1279779

56.614

98.609

0.558
  pilB Legionella pneumophila strain ERS1305867

52.715

99.304

0.523
  pilF Neisseria gonorrhoeae MS11

49.03

98.609

0.483
  pilB Vibrio cholerae strain A1552

47.909

99.826

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

99.304

0.461
  pilB Vibrio campbellii strain DS40M4

45.026

99.652

0.449

Multiple sequence alignment