Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACM0CW_RS18245 Genome accession   NZ_CP180569
Coordinates   3925709..3926818 (+) Length   369 a.a.
NCBI ID   WP_216944735.1    Uniprot ID   -
Organism   Aeromonas caviae strain BJ042     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3920709..3931818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0CW_RS18210 - 3920744..3921574 (+) 831 WP_216944733.1 CPBP family intramembrane glutamic endopeptidase -
  ACM0CW_RS18215 - 3921650..3922069 (-) 420 WP_029313762.1 DUF4426 domain-containing protein -
  ACM0CW_RS18220 yggU 3922088..3922387 (-) 300 WP_010673054.1 DUF167 family protein YggU -
  ACM0CW_RS18225 - 3922387..3922938 (-) 552 WP_042014922.1 YggT family protein -
  ACM0CW_RS18230 proC 3922962..3923786 (-) 825 WP_216944734.1 pyrroline-5-carboxylate reductase -
  ACM0CW_RS18235 - 3923910..3924611 (-) 702 WP_042014924.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACM0CW_RS18240 pilT 3924651..3925685 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  ACM0CW_RS18245 pilU 3925709..3926818 (+) 1110 WP_216944735.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACM0CW_RS18250 yaaA 3926851..3927624 (+) 774 WP_216944736.1 peroxide stress protein YaaA -
  ACM0CW_RS18255 srmB 3927812..3929032 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  ACM0CW_RS18260 - 3929160..3929870 (+) 711 WP_202040292.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ACM0CW_RS18265 brnQ 3930061..3931368 (+) 1308 WP_216944737.1 branched-chain amino acid transport system II carrier protein -
  ACM0CW_RS18270 - 3931458..3931685 (-) 228 WP_039039956.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40887.01 Da        Isoelectric Point: 6.3152

>NTDB_id=1096677 ACM0CW_RS18245 WP_216944735.1 3925709..3926818(+) (pilU) [Aeromonas caviae strain BJ042]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1096677 ACM0CW_RS18245 WP_216944735.1 3925709..3926818(+) (pilU) [Aeromonas caviae strain BJ042]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGACCAGCCCCCCTCGACAAGACGACGGCGCTGGCGCTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTACATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment