Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACM0CZ_RS02530 Genome accession   NZ_CP180559
Coordinates   541400..543106 (-) Length   568 a.a.
NCBI ID   WP_411637631.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BJ014     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 536400..548106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0CZ_RS02500 mutT 537077..537490 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  ACM0CZ_RS02505 yacG 537551..537745 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  ACM0CZ_RS02510 zapD 537755..538477 (-) 723 WP_411637634.1 cell division protein ZapD -
  ACM0CZ_RS02515 coaE 538515..539129 (-) 615 WP_411637633.1 dephospho-CoA kinase -
  ACM0CZ_RS02520 pilD 539148..540020 (-) 873 WP_331414179.1 A24 family peptidase Machinery gene
  ACM0CZ_RS02525 pilC 540043..541284 (-) 1242 WP_411637632.1 type II secretion system F family protein Machinery gene
  ACM0CZ_RS02530 pilB 541400..543106 (-) 1707 WP_411637631.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACM0CZ_RS02535 - 543111..543575 (-) 465 WP_411637630.1 pilin -
  ACM0CZ_RS02540 nadC 543898..544755 (-) 858 WP_282464815.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACM0CZ_RS02545 - 544761..545231 (-) 471 WP_282464816.1 TIGR02281 family clan AA aspartic protease -
  ACM0CZ_RS02550 ampD 545378..545950 (+) 573 WP_324019715.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACM0CZ_RS02555 pdhR 546226..546990 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62553.92 Da        Isoelectric Point: 5.4342

>NTDB_id=1096610 ACM0CZ_RS02530 WP_411637631.1 541400..543106(-) (pilB) [Aeromonas hydrophila strain BJ014]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIIAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLTAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLT
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1096610 ACM0CZ_RS02530 WP_411637631.1 541400..543106(-) (pilB) [Aeromonas hydrophila strain BJ014]
ATGACCTCTAGCCCCAATAGCGGCCTAGCGTTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCTCAGCGTAAACCCTTTGTGACCTTTCTGATCGAAAATGAAATCCTCGACAGCAAAGCATTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACTCAGGGCCACACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAGGCTCTGCTGGTCGAGGAAAACA
AACTCACCACGGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAAGTGTCGGATGAAGGGTCACGCCTCGACGAGAGCGTCAATACGGCCGACGATGACGCGCCGATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATATC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCAACTCCGCCAGTCAATCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCACGGCTCGACATTGCCGAACGGCGACTGCCCCAAGATGGTCGCATCAAACTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGAGTCAATACCCTACCCACCATGTGGGGCGAAAAAATCGTTATCCGTCTGCTCGACTCCTCCG
CCGCACGCCTCAACATTGAGCAGCTTGGTTTTGATGATCGGCAAAAAGCGCAATATCTGCGTGCTCTATCGAAGCCTCAG
GGCATGATCCTGGTAACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTTAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGTGTCAACCAGGTGCAGGTCAATCCCAAAG
CCGGGCTCACCTTCGCCAGCGCCCTGCGCTCTTTCTTGCGCCAGGACCCCGACGTAGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCAGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATTGCTCAAC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGACCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGTTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAGATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGACG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.856

97.887

0.537
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.779

98.944

0.512
  pilF Neisseria gonorrhoeae MS11

46.099

99.296

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

38.98

96.655

0.377
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment