Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYW_RS03615 Genome accession   NZ_CP180555
Coordinates   814685..816400 (-) Length   571 a.a.
NCBI ID   WP_415905246.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD48_55     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 809685..821400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYW_RS03590 (ACMXYW_03590) - 810660..811619 (+) 960 WP_415905241.1 Nudix family hydrolase -
  ACMXYW_RS03595 (ACMXYW_03595) yacG 811597..811824 (-) 228 WP_415905242.1 DNA gyrase inhibitor YacG -
  ACMXYW_RS03600 (ACMXYW_03600) coaE 811824..812426 (-) 603 WP_415905243.1 dephospho-CoA kinase -
  ACMXYW_RS03605 (ACMXYW_03605) pilD 812430..813278 (-) 849 WP_415905244.1 prepilin peptidase Machinery gene
  ACMXYW_RS03610 (ACMXYW_03610) pilC 813448..814668 (-) 1221 WP_415905245.1 type II secretion system F family protein Machinery gene
  ACMXYW_RS03615 (ACMXYW_03615) pilB 814685..816400 (-) 1716 WP_415905246.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYW_RS03620 (ACMXYW_03620) - 816649..817071 (+) 423 WP_415905247.1 pilin -
  ACMXYW_RS03625 (ACMXYW_03625) - 817143..817484 (+) 342 WP_415905248.1 hypothetical protein -
  ACMXYW_RS03630 (ACMXYW_03630) nadC 817569..818414 (-) 846 WP_415905249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMXYW_RS03635 (ACMXYW_03635) ampD 818516..819061 (+) 546 WP_415905250.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMXYW_RS03640 (ACMXYW_03640) - 819062..819769 (-) 708 WP_415905251.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACMXYW_RS03645 (ACMXYW_03645) - 819965..821362 (+) 1398 WP_415905252.1 DUF4856 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63213.02 Da        Isoelectric Point: 4.7698

>NTDB_id=1096534 ACMXYW_RS03615 WP_415905246.1 814685..816400(-) (pilB) [Neptuniibacter sp. QD48_55]
MQPSQPLSGLAKRLVNEGVCSAEVAAEAYLQSREKQQTFISYVIEQRLVSAYRAAAAASEEFGIPLLDLDALDLTNLPQG
LINDSLISKHHALPLQKRDNRLFVAISDPTNIQALDEFKFQCGITTEAIIVEEDKLTRAIETFLEGQDSALDELEDSDLD
NLEISDGSAPEPEEQDSEEDATKDAPIVRFINKILLDSIKNGASDIHFEPYEKSYRIRSRIDGILQEVAKPPSNLAARLS
ARLKVMSQMDISERRVPQDGRIKMKISKKRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGVEALGFADFQKDLYLTTLHR
PQGMVLVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNAKVGLNFAEALRAFLRQDPDVVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCESCKKPTSLPEVTLIEEG
FSNEELDNLTLFEANHDGCSKCNRGYKGRVGIYEMLPMTPEIAEVIMENGNSLDILRVAETQNFQSLRRSGLKKVAEGIT
SLEEMNRVIKT

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1096534 ACMXYW_RS03615 WP_415905246.1 814685..816400(-) (pilB) [Neptuniibacter sp. QD48_55]
TTGCAACCTAGCCAACCATTAAGTGGACTAGCAAAACGACTGGTTAATGAAGGTGTATGTTCAGCTGAAGTTGCAGCAGA
GGCTTATCTTCAATCAAGAGAAAAGCAGCAAACCTTTATTTCATATGTCATTGAACAACGCCTTGTAAGTGCATATAGAG
CCGCCGCAGCAGCATCTGAAGAATTTGGTATTCCTTTATTGGATTTGGATGCACTAGACCTAACCAATTTGCCCCAAGGC
CTAATTAACGATTCTCTTATTTCGAAACATCACGCTCTACCTTTACAGAAACGTGACAACCGCCTATTCGTTGCTATATC
CGACCCCACAAATATACAAGCATTAGATGAGTTTAAGTTTCAGTGCGGTATCACAACTGAAGCTATCATTGTTGAAGAAG
ACAAACTTACACGGGCTATAGAAACATTTTTAGAAGGGCAAGATTCTGCTTTAGATGAATTAGAAGACAGTGATCTAGAT
AATCTAGAAATTTCAGACGGATCTGCTCCAGAGCCTGAAGAACAAGATTCCGAAGAAGATGCCACAAAAGATGCACCTAT
CGTGCGCTTTATTAACAAAATCCTTTTAGATTCTATTAAAAATGGTGCATCCGATATTCATTTCGAGCCTTATGAAAAAA
GCTATCGAATTCGCTCTAGGATTGACGGTATCCTTCAAGAGGTAGCCAAACCCCCTTCCAATCTAGCAGCGAGACTATCT
GCTCGGTTAAAAGTAATGTCGCAAATGGATATTTCTGAAAGGCGCGTACCCCAAGACGGCCGCATTAAAATGAAAATATC
TAAAAAACGCGCCATAGATTTTCGTGTAAATACTCTTCCCACTCTATGGGGTGAAAAAATTGTACTGCGTATACTAGACC
CGAGCAGTGCAAAAATGGGAGTCGAAGCTCTTGGCTTTGCAGATTTCCAAAAAGATCTTTATTTAACAACCCTTCATCGC
CCACAAGGCATGGTATTAGTAACAGGTCCAACAGGAAGCGGTAAAACCGTAAGTCTTTATACAGGCTTAAATATTCTCAA
CACTGAAGAACGGAATATATCCACTGCAGAAGACCCTGTTGAGATCAACTTGGAAGGGATAAACCAAGTACATGTAAATG
CCAAAGTTGGTTTAAATTTCGCGGAAGCTCTTCGTGCATTCCTTCGCCAAGACCCTGACGTAGTAATGGTTGGTGAAATT
CGAGACCTTGAAACGGCCGAAATAGCTATTAAAGCAGCCCAGACAGGACACATGGTATTATCCACGCTACATACAAATAG
CGCTCCAGAAACACTCACTCGTTTACGAAACATGGGGGTTCCTGCATTTAATATAGCCACATCGGTTAGTCTCATCATAG
CTCAACGATTAGGTAGACGTTTATGTGAAAGCTGCAAAAAACCGACTTCTCTTCCAGAAGTAACGCTAATAGAAGAAGGC
TTTAGCAATGAAGAACTTGATAACCTAACTCTCTTTGAGGCTAACCATGATGGCTGCAGCAAGTGCAATCGCGGCTATAA
AGGTCGAGTAGGCATTTATGAAATGCTTCCTATGACGCCAGAAATTGCTGAAGTTATTATGGAGAATGGCAACTCACTAG
ATATACTAAGAGTAGCTGAAACACAGAATTTCCAGAGCCTTAGGCGCTCTGGTTTGAAAAAAGTCGCGGAAGGAATCACC
AGTCTTGAAGAGATGAATCGTGTCATCAAGACCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.144

100

0.581
  pilB Acinetobacter baylyi ADP1

58.407

98.949

0.578
  pilB Legionella pneumophila strain ERS1305867

55.477

99.124

0.55
  pilB Vibrio cholerae strain A1552

55.299

90.893

0.503
  pilB Vibrio campbellii strain DS40M4

53.204

90.193

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

53.411

89.842

0.48
  pilF Neisseria gonorrhoeae MS11

48.663

98.249

0.478

Multiple sequence alignment