Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYU_RS03670 Genome accession   NZ_CP180551
Coordinates   828465..830180 (-) Length   571 a.a.
NCBI ID   WP_415900244.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD48_11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 823465..835180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYU_RS03645 (ACMXYU_03645) - 824346..825305 (+) 960 WP_415900237.1 Nudix family hydrolase -
  ACMXYU_RS03650 (ACMXYU_03650) - 825283..825510 (-) 228 WP_415883654.1 DNA gyrase inhibitor YacG -
  ACMXYU_RS03655 (ACMXYU_03655) coaE 825535..826134 (-) 600 WP_415900238.1 dephospho-CoA kinase -
  ACMXYU_RS03660 (ACMXYU_03660) pilD 826138..827058 (-) 921 WP_415900240.1 prepilin peptidase Machinery gene
  ACMXYU_RS03665 (ACMXYU_03665) pilC 827228..828448 (-) 1221 WP_415900242.1 type II secretion system F family protein Machinery gene
  ACMXYU_RS03670 (ACMXYU_03670) pilB 828465..830180 (-) 1716 WP_415900244.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYU_RS03675 (ACMXYU_03675) - 830429..830854 (+) 426 WP_415900246.1 pilin -
  ACMXYU_RS03680 (ACMXYU_03680) - 830927..831268 (+) 342 WP_415900247.1 hypothetical protein -
  ACMXYU_RS03685 (ACMXYU_03685) nadC 831353..832198 (-) 846 WP_415900249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMXYU_RS03690 (ACMXYU_03690) ampD 832300..832860 (+) 561 WP_415900251.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMXYU_RS03695 (ACMXYU_03695) - 832846..833553 (-) 708 WP_415900253.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACMXYU_RS03700 (ACMXYU_03700) - 833749..835146 (+) 1398 WP_415887132.1 DUF4856 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63136.92 Da        Isoelectric Point: 4.7698

>NTDB_id=1096488 ACMXYU_RS03670 WP_415900244.1 828465..830180(-) (pilB) [Neptuniibacter sp. QD48_11]
MQPSQPLSGLAKRLVNEGVCSAEVAAEASLQSREKQQTFISYVIEQRLVSAYRAAAAASEEFGIPLLDLDALDLTNLPQG
LINDSLISKHHALPLQKRDNRLFVAISDPTNIQALDEFKFQCGITTEAIIVEEDKLTRAIETFLEGQDSALDELEDSDLD
NLEISDGSAPEPEEQDSEEDATKDAPIVRFINKILLDSIKNGASDIHFEPYEKSYRIRSRIDGILQEVAKPPSNLAARLS
ARLKVMSQMDISERRVPQDGRIKMKISKKRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGVEALGFADFQKDLYLTTLHR
PQGMVLVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNAKVGLNFAEALRAFLRQDPDVVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCESCKKPTSLPEVTLIEEG
FSNEELDNLTLFEANHDGCSKCNRGYKGRVGIYEMLPMTPEIAEVIMENGNSLDILRVAETQNFQSLRRSGLKKVAEGIT
SLEEMNRVIKT

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1096488 ACMXYU_RS03670 WP_415900244.1 828465..830180(-) (pilB) [Neptuniibacter sp. QD48_11]
TTGCAACCTAGCCAACCATTAAGTGGACTAGCAAAACGACTGGTTAATGAAGGTGTATGTTCAGCTGAAGTTGCAGCAGA
GGCTTCTCTTCAATCAAGAGAAAAGCAGCAAACCTTTATCTCATATGTCATTGAACAACGCCTTGTAAGTGCATATAGAG
CCGCCGCAGCAGCATCTGAAGAATTTGGTATTCCTTTATTGGATTTGGATGCACTAGACCTAACCAATTTGCCCCAAGGC
CTAATTAACGATTCTCTTATTTCGAAACATCACGCTCTGCCTTTACAGAAACGTGATAACCGCCTATTCGTTGCTATATC
CGACCCCACAAATATACAAGCATTAGATGAGTTTAAGTTTCAATGCGGTATCACAACTGAAGCTATCATTGTTGAAGAAG
ACAAACTTACACGGGCTATAGAAACATTTTTAGAAGGGCAAGATTCTGCTTTAGATGAATTAGAAGATAGTGATCTAGAT
AATCTAGAAATTTCAGACGGATCTGCTCCAGAGCCTGAAGAGCAAGATTCCGAAGAAGACGCTACAAAAGATGCACCTAT
CGTGCGTTTTATTAACAAAATCCTTTTAGATTCCATTAAAAATGGTGCATCCGATATTCATTTCGAGCCTTATGAAAAAA
GCTATCGAATTCGCTCTAGGATTGACGGTATCCTTCAAGAGGTAGCCAAACCCCCTTCCAACCTAGCAGCGAGGCTATCT
GCTCGGTTAAAAGTAATGTCGCAAATGGATATTTCTGAAAGGCGTGTGCCCCAAGACGGCCGCATTAAAATGAAGATATC
TAAAAAACGCGCCATAGATTTTCGTGTAAATACTCTTCCCACTCTATGGGGTGAAAAAATTGTACTTCGTATACTAGACC
CTAGCAGTGCAAAAATGGGAGTCGAAGCTCTGGGCTTTGCAGATTTCCAGAAAGATCTTTATTTAACAACCCTTCATCGC
CCACAAGGCATGGTATTAGTAACAGGTCCAACAGGAAGTGGTAAAACCGTAAGTCTTTATACAGGCTTAAATATTCTCAA
CACTGAAGAACGGAACATATCTACTGCAGAAGACCCTGTTGAGATCAACTTGGAAGGGATAAACCAAGTACATGTAAATG
CCAAAGTTGGTTTAAATTTTGCTGAAGCTCTTCGTGCATTCCTTCGCCAAGACCCTGACGTAGTAATGGTTGGTGAAATT
CGAGACCTTGAAACGGCCGAAATAGCTATTAAAGCAGCCCAGACAGGACACATGGTATTATCCACGCTACATACAAATAG
CGCTCCAGAAACACTCACTCGTTTACGAAATATGGGGGTTCCTGCATTTAATATAGCCACATCGGTTAGTCTCATCATAG
CTCAACGATTAGGTAGGCGTTTATGTGAAAGCTGCAAAAAACCGACTTCTCTTCCTGAAGTCACACTAATAGAAGAAGGC
TTTAGCAATGAAGAACTTGATAATCTAACTCTCTTTGAAGCTAATCATGATGGCTGTAGCAAGTGCAATCGCGGCTATAA
AGGTCGAGTAGGCATTTATGAAATGCTTCCTATGACGCCAGAAATTGCTGAAGTTATTATGGAGAATGGCAACTCACTAG
ATATACTAAGAGTAGCTGAAACACAGAATTTCCAGAGCCTGAGACGCTCTGGTTTGAAAAAAGTCGCGGAAGGAATCACC
AGTCTTGAAGAAATGAACCGAGTCATCAAGACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.144

100

0.581
  pilB Acinetobacter baylyi ADP1

58.407

98.949

0.578
  pilB Legionella pneumophila strain ERS1305867

55.477

99.124

0.55
  pilB Vibrio cholerae strain A1552

55.299

90.893

0.503
  pilB Vibrio campbellii strain DS40M4

53.204

90.193

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

53.411

89.842

0.48
  pilF Neisseria gonorrhoeae MS11

48.485

98.249

0.476

Multiple sequence alignment