Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYO_RS03715 Genome accession   NZ_CP180550
Coordinates   829453..831168 (-) Length   571 a.a.
NCBI ID   WP_415887126.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD37_6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 824453..836168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYO_RS03690 (ACMXYO_03690) - 825585..826544 (+) 960 WP_415887120.1 Nudix family hydrolase -
  ACMXYO_RS03695 (ACMXYO_03695) - 826522..826749 (-) 228 WP_415887121.1 DNA gyrase inhibitor YacG -
  ACMXYO_RS03700 (ACMXYO_03700) coaE 826749..827351 (-) 603 WP_415887123.1 dephospho-CoA kinase -
  ACMXYO_RS03705 (ACMXYO_03705) pilD 827355..828203 (-) 849 WP_415887124.1 prepilin peptidase Machinery gene
  ACMXYO_RS03710 (ACMXYO_03710) pilC 828216..829436 (-) 1221 WP_415887125.1 type II secretion system F family protein Machinery gene
  ACMXYO_RS03715 (ACMXYO_03715) pilB 829453..831168 (-) 1716 WP_415887126.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYO_RS03720 (ACMXYO_03720) - 831417..831932 (+) 516 WP_415887127.1 pilin -
  ACMXYO_RS03725 (ACMXYO_03725) - 832111..832452 (+) 342 WP_415887128.1 hypothetical protein -
  ACMXYO_RS03730 (ACMXYO_03730) nadC 832535..833380 (-) 846 WP_415887129.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMXYO_RS03735 (ACMXYO_03735) ampD 833482..834027 (+) 546 WP_415887130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMXYO_RS03740 (ACMXYO_03740) - 834028..834735 (-) 708 WP_415887131.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63096.98 Da        Isoelectric Point: 4.8511

>NTDB_id=1096465 ACMXYO_RS03715 WP_415887126.1 829453..831168(-) (pilB) [Neptuniibacter sp. QD37_6]
MQPSQPLSGLAKRLVNEGVCSAEVAADASLQSREKQQTFISYVIEQRLVSAYRAAAAASEEFGMPLLDLDALDPASLPQG
LINDSLISKHHALPLQKRDNRLFVAISDPTNIQALDEFKFQSGITTEAIIVEEDKLTRAIEVFLEGQDSALGELEDSDLD
NLEISDGSVPEQEEQESEEDATKDAPIVRFINKILLDSIKNGASDIHFEPYEKSYRIRSRIDGILQEVAKPPSNLAARLS
ARLKVMSQMDISERRVPQDGRIKMKISKKRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGVEALGFADFQKDLYLTTLHR
PQGMVLVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNAKVGLNFAEALRAFLRQDPDVVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCESCKKPVTLPEVTLREEG
FTEEEISNLSLFEPNQDGCKKCNRGYKGRVGIYEMLPMTSEIAEVIMENGNSLDILKVAEAQNFQSLRRSGLKKVAEGIT
SLEEMNRVIKT

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1096465 ACMXYO_RS03715 WP_415887126.1 829453..831168(-) (pilB) [Neptuniibacter sp. QD37_6]
TTGCAACCTAGCCAACCATTAAGCGGACTAGCCAAACGATTGGTTAACGAAGGTGTATGTTCAGCCGAAGTTGCAGCAGA
CGCATCTCTTCAATCCAGAGAAAAACAACAAACCTTCATTTCATACGTTATCGAACAACGGTTAGTAAGTGCATACAGAG
CCGCTGCCGCTGCATCCGAAGAATTCGGCATGCCTCTACTAGACTTAGACGCTCTGGATCCCGCTAGCCTCCCCCAAGGG
CTTATTAACGATTCCCTTATTTCAAAACATCATGCACTTCCCTTACAAAAACGCGACAACAGACTCTTTGTAGCCATTTC
TGATCCTACAAACATCCAAGCGCTTGATGAATTTAAGTTTCAAAGCGGCATTACCACTGAAGCTATTATCGTTGAAGAGG
ATAAATTAACCCGTGCAATTGAAGTATTTCTAGAAGGTCAAGATTCCGCTTTAGGTGAATTAGAAGATAGCGACCTAGAT
AATCTCGAAATCTCAGATGGAAGCGTTCCTGAACAGGAAGAACAAGAATCCGAAGAAGACGCTACCAAAGACGCGCCTAT
TGTACGTTTTATTAACAAAATACTTTTAGATTCCATTAAAAATGGTGCGTCTGATATTCATTTTGAACCCTATGAAAAAA
GTTACCGCATCAGAAGCAGGATAGATGGAATCCTACAAGAAGTCGCCAAACCCCCATCTAACCTTGCTGCCAGGCTGTCA
GCTCGTTTAAAAGTAATGTCACAAATGGATATTTCAGAAAGGCGCGTCCCTCAAGACGGCCGCATTAAAATGAAGATATC
CAAAAAGCGTGCAATAGATTTCCGTGTGAATACCCTACCTACTCTGTGGGGTGAGAAGATTGTGCTTCGTATACTTGACC
CTAGCAGCGCCAAAATGGGGGTAGAAGCGCTTGGCTTTGCCGATTTTCAAAAAGATCTGTATCTAACCACCCTCCATCGT
CCACAAGGCATGGTACTCGTAACAGGCCCTACAGGTAGCGGTAAAACGGTGAGCCTCTATACAGGCTTAAATATTCTCAA
TACCGAAGAGCGCAATATCTCTACAGCAGAAGACCCTGTTGAGATTAACCTAGAAGGTATCAATCAGGTTCATGTAAACG
CCAAAGTAGGCTTAAATTTCGCAGAAGCTCTACGAGCATTCCTTCGCCAAGACCCTGATGTCGTAATGGTTGGTGAGATT
CGAGACCTTGAAACAGCAGAAATCGCCATAAAAGCAGCACAAACGGGTCACATGGTACTATCTACACTTCACACCAACAG
TGCTCCAGAAACTTTGACGCGTTTACGTAACATGGGCGTACCAGCCTTCAATATAGCGACATCAGTAAGCCTCATCATTG
CTCAGCGACTAGGCAGGCGACTCTGTGAAAGCTGTAAAAAACCAGTAACCCTCCCTGAAGTAACCTTAAGAGAAGAAGGC
TTCACAGAAGAAGAAATAAGCAATCTAAGTTTATTTGAGCCAAACCAAGATGGCTGCAAAAAATGCAATAGAGGCTATAA
AGGCAGGGTCGGTATTTATGAAATGCTACCAATGACTTCAGAAATTGCTGAAGTAATTATGGAGAATGGTAACTCTCTAG
ATATACTAAAAGTAGCCGAAGCTCAAAACTTCCAAAGCCTACGTCGCTCAGGATTAAAGAAAGTCGCGGAAGGAATCACC
AGTCTTGAAGAAATGAACAGAGTCATCAAGACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.494

100

0.585
  pilB Acinetobacter baylyi ADP1

58.407

98.949

0.578
  pilB Legionella pneumophila strain ERS1305867

54.947

99.124

0.545
  pilB Vibrio cholerae strain A1552

55.513

90.543

0.503
  pilB Vibrio campbellii strain DS40M4

53.876

90.368

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

53.606

89.842

0.482
  pilF Neisseria gonorrhoeae MS11

48.754

98.424

0.48

Multiple sequence alignment