Detailed information NTG1096450

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMXYS_RS14490
• Genome accession: NZ_CP180549
• Coordinates: 3186013..3187185 (+)
• Length: 390 a.a.
• NCBI ID: WP_415882381.1
• Uniprot ID: -
• Organism: Neptuniibacter sp. QD57_21
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3181013..3192185

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMXYS_RS14470 (ACMXYS_14470)	-	3182306..3183076 (-)	771	WP_415896948.1	metal ABC transporter permease	-
ACMXYS_RS14475 (ACMXYS_14475)	-	3183076..3183975 (-)	900	WP_415896949.1	metal ABC transporter substrate-binding protein	-
ACMXYS_RS14480 (ACMXYS_14480)	-	3183956..3185092 (-)	1137	WP_415896950.1	hypothetical protein	-
ACMXYS_RS14485 (ACMXYS_14485)	-	3185208..3185861 (-)	654	WP_415882380.1	DUF1826 domain-containing protein	-
ACMXYS_RS14490 (ACMXYS_14490)	pilU	3186013..3187185 (+)	1173	WP_415882381.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMXYS_RS14495 (ACMXYS_14495)	-	3187276..3188421 (-)	1146	WP_415882382.1	hypothetical protein	-
ACMXYS_RS14500 (ACMXYS_14500)	-	3188704..3189135 (+)	432	WP_415882383.1	hypothetical protein	-
ACMXYS_RS14505 (ACMXYS_14505)	-	3189140..3189523 (+)	384	WP_415882384.1	hypothetical protein	-
ACMXYS_RS14510 (ACMXYS_14510)	fadA	3190217..3191392 (-)	1176	WP_415895155.1	acetyl-CoA C-acyltransferase FadA	-

Sequence

Protein

• Length: 390 a.a.
• Molecular weight: 43588.71 Da
• Isoelectric Point: 5.2319

>NTDB_id=1096450 ACMXYS_RS14490 WP_415882381.1 3186013..3187185(+) (pilU) [Neptuniibacter sp. QD57_21]
MEFKDYLTILAKRDGSDLYLTTGAPPCAKFEGKLTPLSKEPYKPGEIEAIANSLMDEQQRATFEEELEMNLAYSIPKVAR
YRINIFKQRNQYSLVARNIKADIPKMEDLGLPPVLKDVIMEKRGLVLFVGATGSGKSTSLAALIDHRNRNSGGHIITIED
PIEFVHNHRKSLINQREVGVDTRNFHSALKNALRQAPDVILIGEIRDQETMEQCLTYAETGHLAISTLHANNANQAIDRI
INFFPQARHKQIFHDLALNLKAVVSQRLIPTIEGKRCAAIEILLGTSSIQELIMKGDVKGIKEVMSKGSDTTGMQTFDDS
LFKLFDEGKITQDQAIVNADSANDMRLRMNLSVVDEKTEKTDAAPEPEKQSFDEDNPLAGIKLLDEDEEV

Nucleotide

• Length: 1173 bp

>NTDB_id=1096450 ACMXYS_RS14490 WP_415882381.1 3186013..3187185(+) (pilU) [Neptuniibacter sp. QD57_21]
ATGGAATTTAAGGATTACCTTACGATTTTGGCGAAACGTGACGGCTCCGACCTTTACCTAACTACCGGCGCTCCACCTTG
TGCAAAGTTTGAAGGTAAATTAACGCCATTAAGTAAAGAGCCTTATAAACCTGGTGAGATCGAAGCAATCGCAAACTCCT
TAATGGATGAGCAGCAACGCGCCACCTTCGAAGAAGAGTTAGAGATGAACCTCGCTTACTCTATTCCCAAAGTAGCGCGT
TACCGTATCAACATCTTTAAGCAACGAAACCAATACTCTCTGGTCGCGCGTAATATCAAAGCCGATATTCCTAAGATGGA
AGACTTAGGCCTACCGCCCGTCCTTAAAGATGTGATTATGGAAAAACGCGGCTTAGTACTGTTTGTTGGCGCGACAGGTT
CGGGTAAATCAACATCTCTTGCTGCACTGATTGATCACCGTAACCGTAATAGCGGTGGGCATATCATCACCATCGAAGAC
CCGATCGAGTTTGTTCACAACCACCGAAAGTCGCTGATCAATCAGCGTGAAGTCGGGGTTGATACCCGTAACTTCCATAG
CGCTCTGAAAAACGCTCTGCGCCAAGCACCCGATGTAATTTTGATCGGCGAGATCCGAGATCAGGAAACCATGGAACAGT
GCCTGACCTATGCTGAAACGGGTCACTTAGCGATCTCCACGCTACACGCGAACAACGCTAACCAAGCGATTGATCGTATT
ATTAACTTCTTCCCACAAGCACGACATAAACAGATCTTCCACGACCTTGCGCTTAACCTCAAAGCCGTGGTTTCTCAGCG
TCTGATTCCAACCATTGAAGGTAAACGCTGTGCAGCCATTGAGATACTTCTAGGTACTTCCAGTATCCAAGAACTGATCA
TGAAAGGCGATGTAAAAGGGATCAAAGAGGTAATGTCTAAGGGTTCTGATACCACTGGCATGCAAACCTTTGATGACTCC
CTGTTCAAACTCTTTGACGAAGGTAAGATCACGCAAGACCAAGCTATTGTTAATGCCGACTCAGCAAACGATATGCGCCT
TCGCATGAACCTTTCGGTGGTGGATGAAAAAACTGAAAAAACCGATGCAGCACCTGAACCCGAAAAACAATCCTTCGATG
AAGATAATCCGCTAGCTGGGATTAAGCTATTGGATGAAGATGAAGAGGTTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.541	90.513	0.485
pilU	Acinetobacter baylyi ADP1	51.695	90.769	0.469
pilU	Vibrio cholerae strain A1552	46.286	89.744	0.415
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.507	94.103	0.4
pilT	Pseudomonas aeruginosa PAK	42.687	85.897	0.367
pilT	Pseudomonas stutzeri DSM 10701	42.09	85.897	0.362