Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYS_RS03715 Genome accession   NZ_CP180549
Coordinates   861366..863081 (-) Length   571 a.a.
NCBI ID   WP_415897622.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD57_21     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 856366..868081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYS_RS03690 (ACMXYS_03690) - 857498..858457 (+) 960 WP_415897617.1 Nudix family hydrolase -
  ACMXYS_RS03695 (ACMXYS_03695) yacG 858435..858662 (-) 228 WP_415897618.1 DNA gyrase inhibitor YacG -
  ACMXYS_RS03700 (ACMXYS_03700) coaE 858662..859264 (-) 603 WP_415897619.1 dephospho-CoA kinase -
  ACMXYS_RS03705 (ACMXYS_03705) pilD 859268..860116 (-) 849 WP_415897620.1 prepilin peptidase Machinery gene
  ACMXYS_RS03710 (ACMXYS_03710) pilC 860129..861349 (-) 1221 WP_415897621.1 type II secretion system F family protein Machinery gene
  ACMXYS_RS03715 (ACMXYS_03715) pilB 861366..863081 (-) 1716 WP_415897622.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYS_RS03720 (ACMXYS_03720) - 863300..863809 (+) 510 WP_415897623.1 pilin -
  ACMXYS_RS03725 (ACMXYS_03725) - 863929..864075 (+) 147 WP_415897624.1 hypothetical protein -
  ACMXYS_RS03730 (ACMXYS_03730) - 864234..864575 (+) 342 WP_415897625.1 hypothetical protein -
  ACMXYS_RS03735 (ACMXYS_03735) nadC 864663..865508 (-) 846 WP_415897626.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMXYS_RS03740 (ACMXYS_03740) ampD 865610..866155 (+) 546 WP_415897627.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMXYS_RS03745 (ACMXYS_03745) - 866156..866863 (-) 708 WP_415883663.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63018.82 Da        Isoelectric Point: 4.8083

>NTDB_id=1096442 ACMXYS_RS03715 WP_415897622.1 861366..863081(-) (pilB) [Neptuniibacter sp. QD57_21]
MQPSQPLSGLAKRLVNEGVCSAEVAADASLQSREKQQTFISYVIEQRLVSAYRAAAAASEEFGMPLLDLDALDPASLPQG
LINDSLITKHHALPLQKRDNRLFVAISDPTNIQALDEFKFQSGITTEAIIVEEDKLTRAIESFLEGQDSALDELDDSDLD
NLEVSDGSTPDKEEQESEEDATKDAPIVRFINKILLDSIKNGASDIHFEPYEKSYRIRSRIDGILQEVAKPPSNLAARLS
ARLKVMSQMDISEKRVPQDGRIKMKISKKRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGVEALGFADFQKELYLGTLHK
PQGMVLVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNAKVGLNFAEALRAFLRQDPDVVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCESCKKPITLPEVTLREEG
FTDEELSSLTLFEANQDGCSKCNRGYKGRVGIYEMLPMTPEIAEVIMENGNSLDILRVAETQNFQSLRRSGLKKVAEGIT
SLEEMNRVIKT

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1096442 ACMXYS_RS03715 WP_415897622.1 861366..863081(-) (pilB) [Neptuniibacter sp. QD57_21]
TTGCAGCCAAGCCAACCTTTAAGTGGACTTGCCAAGCGTTTAGTGAATGAAGGCGTATGTTCTGCAGAAGTTGCAGCTGA
TGCTTCGCTACAATCCCGAGAGAAACAACAAACGTTTATCTCTTATGTTATTGAACAGCGCTTAGTCAGTGCATATAGGG
CTGCAGCTGCAGCATCCGAAGAGTTTGGTATGCCTCTTTTGGATTTGGACGCCTTAGACCCAGCATCTCTTCCTCAAGGA
CTAATTAACGATTCCCTTATAACTAAACACCATGCGCTCCCCCTGCAGAAACGTGACAATCGCCTATTTGTAGCTATTTC
TGACCCCACTAACATACAAGCGTTAGATGAATTTAAGTTTCAAAGCGGCATTACAACAGAAGCGATCATTGTCGAGGAGG
ATAAACTCACTCGCGCCATAGAGAGTTTCCTTGAAGGCCAAGATTCAGCCTTAGATGAATTAGATGATAGTGATTTAGAT
AATCTCGAAGTATCAGATGGCTCTACGCCAGATAAAGAAGAGCAAGAAAGCGAAGAAGACGCAACAAAAGATGCACCAAT
TGTTCGCTTTATCAATAAAATCCTCCTAGATTCTATAAAAAATGGCGCATCTGATATCCACTTTGAGCCCTACGAAAAAA
GCTACCGCATCAGAAGCAGGATCGATGGAATTCTACAGGAAGTTGCAAAGCCACCTTCAAACCTTGCTGCACGGCTTTCT
GCTCGCTTAAAAGTCATGTCGCAGATGGATATTTCTGAGAAACGTGTACCTCAGGATGGCCGCATTAAAATGAAGATATC
CAAGAAACGCGCTATCGACTTTCGAGTAAACACCCTTCCCACCCTTTGGGGAGAGAAAATCGTACTACGTATTCTTGACC
CTAGCAGCGCAAAAATGGGGGTAGAAGCCTTAGGTTTTGCCGACTTCCAAAAAGAACTTTACTTAGGCACACTCCACAAA
CCACAAGGCATGGTATTGGTAACAGGGCCAACAGGTAGTGGTAAAACTGTAAGTCTTTATACCGGTTTAAATATCCTTAA
TACCGAAGAGCGGAATATTTCTACAGCAGAAGACCCTGTAGAAATCAACTTGGAAGGCATAAATCAAGTCCATGTAAATG
CAAAAGTGGGGTTAAATTTTGCTGAAGCTCTACGCGCATTCCTTCGTCAAGACCCTGATGTGGTAATGGTGGGTGAGATC
CGAGACTTAGAAACAGCGGAGATCGCCATAAAAGCAGCCCAAACAGGTCACATGGTGCTATCTACACTTCATACAAATAG
CGCGCCAGAAACGCTTACACGTTTACGTAATATGGGCGTGCCAGCCTTTAACATCGCTACATCTGTCAGTCTTATCATTG
CCCAACGTTTAGGGCGACGCTTATGTGAAAGTTGCAAAAAACCTATCACCCTCCCTGAAGTTACGTTAAGGGAGGAAGGC
TTTACTGATGAAGAGTTAAGCTCACTAACTCTATTTGAAGCGAATCAAGATGGCTGCAGCAAATGCAATAGAGGCTATAA
AGGACGAGTAGGTATCTATGAAATGCTACCTATGACACCAGAAATTGCTGAAGTAATTATGGAGAATGGTAACTCTCTAG
ATATACTTAGAGTAGCTGAAACACAAAACTTCCAGAGCCTACGACGCTCTGGGTTAAAGAAGGTCGCGGAAGGAATCACC
AGTCTTGAAGAGATGAACCGAGTCATCAAGACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.646

98.949

0.59
  pilB Acinetobacter baumannii D1279779

58.524

99.65

0.583
  pilB Legionella pneumophila strain ERS1305867

54.049

99.475

0.538
  pilB Vibrio cholerae strain A1552

55.319

90.543

0.501
  pilB Vibrio campbellii strain DS40M4

53.682

90.368

0.485
  pilF Neisseria gonorrhoeae MS11

49.198

98.249

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

53.411

89.842

0.48

Multiple sequence alignment