Detailed information NTG1096404

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMXYP_RS14690
• Genome accession: NZ_CP180546
• Coordinates: 3220586..3221758 (+)
• Length: 390 a.a.
• NCBI ID: WP_415882381.1
• Uniprot ID: -
• Organism: Neptuniibacter sp. QD34_54
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3215586..3226758

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMXYP_RS14670 (ACMXYP_14670)	-	3216906..3217676 (-)	771	WP_415882377.1	metal ABC transporter permease	-
ACMXYP_RS14675 (ACMXYP_14675)	-	3217676..3218575 (-)	900	WP_415882378.1	metal ABC transporter substrate-binding protein	-
ACMXYP_RS14680 (ACMXYP_14680)	-	3218556..3219665 (-)	1110	WP_415882379.1	hypothetical protein	-
ACMXYP_RS14685 (ACMXYP_14685)	-	3219781..3220434 (-)	654	WP_415882380.1	DUF1826 domain-containing protein	-
ACMXYP_RS14690 (ACMXYP_14690)	pilU	3220586..3221758 (+)	1173	WP_415882381.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMXYP_RS14695 (ACMXYP_14695)	-	3221849..3222994 (-)	1146	WP_415882382.1	hypothetical protein	-
ACMXYP_RS14700 (ACMXYP_14700)	-	3223277..3223708 (+)	432	WP_415882383.1	hypothetical protein	-
ACMXYP_RS14705 (ACMXYP_14705)	-	3223713..3224096 (+)	384	WP_415882384.1	hypothetical protein	-
ACMXYP_RS14710 (ACMXYP_14710)	fadA	3224790..3225965 (-)	1176	WP_415882385.1	acetyl-CoA C-acyltransferase FadA	-

Sequence

Protein

• Length: 390 a.a.
• Molecular weight: 43588.71 Da
• Isoelectric Point: 5.2319

>NTDB_id=1096404 ACMXYP_RS14690 WP_415882381.1 3220586..3221758(+) (pilU) [Neptuniibacter sp. QD34_54]
MEFKDYLTILAKRDGSDLYLTTGAPPCAKFEGKLTPLSKEPYKPGEIEAIANSLMDEQQRATFEEELEMNLAYSIPKVAR
YRINIFKQRNQYSLVARNIKADIPKMEDLGLPPVLKDVIMEKRGLVLFVGATGSGKSTSLAALIDHRNRNSGGHIITIED
PIEFVHNHRKSLINQREVGVDTRNFHSALKNALRQAPDVILIGEIRDQETMEQCLTYAETGHLAISTLHANNANQAIDRI
INFFPQARHKQIFHDLALNLKAVVSQRLIPTIEGKRCAAIEILLGTSSIQELIMKGDVKGIKEVMSKGSDTTGMQTFDDS
LFKLFDEGKITQDQAIVNADSANDMRLRMNLSVVDEKTEKTDAAPEPEKQSFDEDNPLAGIKLLDEDEEV

Nucleotide

• Length: 1173 bp

>NTDB_id=1096404 ACMXYP_RS14690 WP_415882381.1 3220586..3221758(+) (pilU) [Neptuniibacter sp. QD34_54]
ATGGAATTTAAGGATTACCTTACGATTTTGGCGAAACGTGACGGCTCCGACCTTTACCTAACTACCGGCGCTCCACCTTG
TGCAAAGTTTGAAGGTAAATTAACGCCATTAAGTAAAGAGCCTTATAAACCTGGTGAGATCGAAGCAATCGCAAACTCCT
TAATGGATGAGCAGCAACGCGCCACCTTCGAAGAAGAGTTAGAGATGAACCTCGCTTACTCTATTCCCAAAGTAGCGCGT
TACCGTATCAACATCTTTAAGCAACGAAACCAATACTCTCTGGTCGCGCGTAATATCAAAGCCGATATTCCTAAGATGGA
AGACTTAGGCCTACCGCCCGTCCTTAAAGATGTGATTATGGAAAAACGCGGCTTAGTACTGTTTGTTGGCGCGACAGGTT
CGGGTAAATCAACATCTCTTGCTGCACTGATTGATCACCGTAACCGTAATAGCGGTGGGCATATCATCACCATCGAAGAC
CCGATCGAGTTTGTTCACAACCACCGAAAGTCGCTGATCAATCAGCGTGAAGTCGGGGTTGATACCCGTAACTTCCATAG
CGCTCTGAAAAACGCTCTGCGCCAAGCACCCGATGTAATTTTGATCGGCGAGATCCGAGATCAGGAAACCATGGAACAGT
GCCTGACCTATGCTGAAACGGGTCACTTAGCGATCTCCACGCTACACGCGAACAACGCTAACCAAGCGATTGATCGTATT
ATTAACTTCTTCCCACAAGCACGACATAAACAGATCTTCCACGACCTTGCGCTTAACCTCAAAGCCGTGGTTTCTCAGCG
TCTGATTCCAACCATTGAAGGTAAACGCTGTGCAGCCATTGAGATACTTCTAGGTACTTCCAGTATCCAAGAACTGATCA
TGAAAGGCGATGTAAAAGGGATCAAAGAGGTAATGTCTAAGGGTTCTGATACCACTGGCATGCAAACCTTTGATGACTCC
CTGTTCAAACTCTTTGACGAAGGTAAGATCACGCAAGACCAAGCTATTGTTAATGCCGACTCAGCAAACGATATGCGCCT
TCGCATGAACCTTTCGGTGGTGGATGAAAAAACTGAAAAAACCGATGCAGCACCTGAACCCGAAAAACAATCCTTCGATG
AAGATAATCCGCTAGCTGGGATTAAGCTATTGGATGAAGATGAAGAGGTTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.541	90.513	0.485
pilU	Acinetobacter baylyi ADP1	51.695	90.769	0.469
pilU	Vibrio cholerae strain A1552	46.286	89.744	0.415
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.507	94.103	0.4
pilT	Pseudomonas aeruginosa PAK	42.687	85.897	0.367
pilT	Pseudomonas stutzeri DSM 10701	42.09	85.897	0.362