Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYP_RS03680 Genome accession   NZ_CP180546
Coordinates   858422..860137 (-) Length   571 a.a.
NCBI ID   WP_415883658.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD34_54     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 853422..865137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYP_RS03655 (ACMXYP_03655) - 854553..855512 (+) 960 WP_415883653.1 Nudix family hydrolase -
  ACMXYP_RS03660 (ACMXYP_03660) - 855490..855717 (-) 228 WP_415883654.1 DNA gyrase inhibitor YacG -
  ACMXYP_RS03665 (ACMXYP_03665) coaE 855714..856319 (-) 606 WP_415883655.1 dephospho-CoA kinase -
  ACMXYP_RS03670 (ACMXYP_03670) pilD 856323..857171 (-) 849 WP_415883656.1 prepilin peptidase Machinery gene
  ACMXYP_RS03675 (ACMXYP_03675) pilC 857185..858405 (-) 1221 WP_415883657.1 type II secretion system F family protein Machinery gene
  ACMXYP_RS03680 (ACMXYP_03680) pilB 858422..860137 (-) 1716 WP_415883658.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYP_RS03685 (ACMXYP_03685) - 860381..860902 (+) 522 WP_415883659.1 pilin -
  ACMXYP_RS03690 (ACMXYP_03690) - 861075..861416 (+) 342 WP_415883660.1 hypothetical protein -
  ACMXYP_RS03695 (ACMXYP_03695) nadC 861501..862346 (-) 846 WP_415883661.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMXYP_RS03700 (ACMXYP_03700) ampD 862448..862993 (+) 546 WP_415883662.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMXYP_RS03705 (ACMXYP_03705) - 862995..863702 (-) 708 WP_415883663.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63236.01 Da        Isoelectric Point: 4.7722

>NTDB_id=1096396 ACMXYP_RS03680 WP_415883658.1 858422..860137(-) (pilB) [Neptuniibacter sp. QD34_54]
MQPSQPLSGLAKRLVNEGVCSAEVAADASIQSREKQQTFISYVIEQRLVSAYRAAAAASEEFGMPLLDLDALDLNSLPQG
LINDTLITKHHALPLQKRDNRLFVAISDPTNIQALDEFKFQSGITTEAIIVEEDKLTRAIETFLEGQDSALDELEDSDLD
NLEISDGSAPDQEEKESEEDATKDAPIVRFINKILLDSIKNGASDIHFEPYEKSYRIRSRIDGILQEVAKPPSNLAARLS
ARLKVMSQMDISERRVPQDGRIKMQISKKRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGVEALGFADFQKDLYLTTLHR
PQGMVLVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNAKVGLNFAEALRAFLRQDPDVVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCEACKRPVTLPEVTLKEEG
FTDEEINDLTLFEPNHDGCSKCNRGYKGRVGIYEMLPMSPEIAEVIMENGNSLDILRVAETQNFQSLRRSGLKKVAEGIT
SLEEMNRVIKT

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1096396 ACMXYP_RS03680 WP_415883658.1 858422..860137(-) (pilB) [Neptuniibacter sp. QD34_54]
TTGCAACCTAGTCAACCACTAAGCGGACTTGCCAAACGACTAGTCAACGAAGGCGTTTGTTCTGCAGAAGTTGCTGCAGA
TGCTTCTATACAATCAAGAGAAAAACAACAAACCTTCATTTCCTACGTCATTGAGCAAAGATTAGTAAGTGCATACCGTG
CGGCAGCGGCAGCATCTGAAGAGTTTGGCATGCCACTTTTAGATCTCGATGCATTAGATCTAAACAGTTTGCCTCAAGGC
TTAATCAACGACACTCTGATCACTAAGCACCATGCACTCCCTCTGCAAAAACGTGACAACCGTCTATTCGTAGCGATTTC
TGATCCTACAAATATTCAAGCCTTAGATGAGTTCAAATTTCAAAGTGGTATCACCACTGAAGCGATCATTGTTGAAGAAG
ATAAACTCACACGAGCTATTGAAACCTTCTTAGAAGGTCAGGATTCAGCTTTAGATGAGTTAGAAGATAGTGATTTAGAT
AATTTGGAGATCTCTGATGGGTCAGCGCCTGACCAAGAAGAAAAAGAGAGTGAAGAAGACGCTACCAAAGATGCGCCTAT
TGTCCGATTTATCAATAAGATTCTTCTAGATTCAATAAAGAATGGCGCTTCCGACATTCACTTCGAACCTTATGAAAAAA
GCTATCGAATCAGAAGCAGGATTGATGGCATTCTACAAGAAGTTGCTAAACCACCATCTAATCTAGCAGCTAGACTTTCC
GCACGATTAAAAGTTATGTCCCAAATGGACATTTCCGAGCGGCGTGTCCCCCAAGATGGCCGTATCAAAATGCAAATATC
TAAAAAACGGGCCATCGATTTTCGTGTAAATACACTTCCGACACTTTGGGGTGAAAAGATTGTACTTCGTATTCTTGACC
CTAGCAGCGCAAAAATGGGCGTAGAAGCATTAGGCTTTGCTGATTTCCAGAAAGATCTATACCTAACAACTCTGCACAGA
CCGCAGGGAATGGTGCTAGTAACAGGTCCAACAGGTAGCGGTAAAACGGTTAGTCTTTACACAGGTCTTAACATTCTCAA
TACAGAAGAACGAAACATTTCCACTGCAGAAGATCCTGTAGAGATCAATTTAGAAGGGATTAACCAAGTACATGTAAATG
CTAAAGTCGGACTTAATTTTGCTGAAGCACTTCGTGCATTCCTTCGCCAAGACCCCGATGTAGTAATGGTTGGTGAGATC
CGAGATTTGGAAACAGCTGAAATAGCCATTAAAGCCGCCCAAACGGGTCATATGGTTTTATCAACACTTCACACCAATAG
TGCGCCAGAAACACTGACTCGATTGAGAAACATGGGCGTACCAGCATTTAATATTGCGACCTCTGTAAGTCTTATTATTG
CTCAACGATTAGGTCGACGCCTATGTGAAGCCTGTAAACGCCCGGTAACGTTACCAGAAGTAACTCTCAAGGAAGAAGGG
TTTACTGACGAAGAAATTAATGATTTAACTCTATTTGAACCTAATCATGATGGTTGCAGTAAATGCAACCGAGGTTACAA
AGGAAGGGTCGGTATCTATGAAATGCTACCTATGTCACCAGAAATTGCTGAAGTTATTATGGAGAATGGCAACTCTCTGG
ATATACTTAGGGTAGCTGAAACACAAAACTTCCAGAGTTTAAGACGCTCGGGATTGAAGAAAGTCGCTGAAGGAATCACA
AGTCTTGAAGAGATGAATCGTGTCATCAAGACCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.319

100

0.583
  pilB Acinetobacter baylyi ADP1

58.407

98.949

0.578
  pilB Legionella pneumophila strain ERS1305867

54.145

99.299

0.538
  pilB Vibrio cholerae strain A1552

55.513

90.543

0.503
  pilF Neisseria gonorrhoeae MS11

49.554

98.249

0.487
  pilB Vibrio campbellii strain DS40M4

53.295

90.368

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

53.021

89.842

0.476

Multiple sequence alignment