Detailed information NTG1096381

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMXYN_RS14795
• Genome accession: NZ_CP180545
• Coordinates: 3259842..3261014 (+)
• Length: 390 a.a.
• NCBI ID: WP_415892496.1
• Uniprot ID: -
• Organism: Neptuniibacter sp. PT8_73
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3254842..3266014

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMXYN_RS14775 (ACMXYN_14775)	-	3256141..3256911 (-)	771	WP_415892489.1	metal ABC transporter permease	-
ACMXYN_RS14780 (ACMXYN_14780)	-	3256911..3257810 (-)	900	WP_415892491.1	metal ABC transporter substrate-binding protein	-
ACMXYN_RS14785 (ACMXYN_14785)	-	3257791..3258921 (-)	1131	WP_415892492.1	hypothetical protein	-
ACMXYN_RS14790 (ACMXYN_14790)	-	3259037..3259690 (-)	654	WP_415892494.1	DUF1826 domain-containing protein	-
ACMXYN_RS14795 (ACMXYN_14795)	pilU	3259842..3261014 (+)	1173	WP_415892496.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMXYN_RS14800 (ACMXYN_14800)	-	3261223..3261606 (+)	384	WP_415892498.1	hypothetical protein	-
ACMXYN_RS14805 (ACMXYN_14805)	-	3261718..3262461 (+)	744	WP_415892499.1	SDR family oxidoreductase	-
ACMXYN_RS14810 (ACMXYN_14810)	fadA	3262630..3263805 (-)	1176	WP_415892500.1	acetyl-CoA C-acyltransferase FadA	-
ACMXYN_RS14815 (ACMXYN_14815)	fadB	3263818..3265980 (-)	2163	WP_415889265.1	fatty acid oxidation complex subunit alpha FadB	-

Sequence

Protein

• Length: 390 a.a.
• Molecular weight: 43558.68 Da
• Isoelectric Point: 5.2319

>NTDB_id=1096381 ACMXYN_RS14795 WP_415892496.1 3259842..3261014(+) (pilU) [Neptuniibacter sp. PT8_73]
MEFKDYLTILAKRDGSDLYLTTGAPPCAKFEGKLTPLSKEPYKPGEIEAIANSLMDEQQRATFEEELEMNLAYSIPKVAR
YRINIFKQRNQYSLVARNIKADIPKMEDLGLPPVLKDVIMEKRGLVLFVGATGSGKSTSLAALIDHRNRNSGGHIITIED
PIEFVHNHRKSLINQREVGVDTRNFHSALKNALRQAPDVILIGEIRDQETMEQCLTYAETGHLAISTLHANNANQAIDRI
INFFPQARHKQIFHDLALNLKAVVSQRLIPTIEGKRCAAIEILLGTSSIQELIMKGDVKGIKEVMSKGSDTTGMQTFDDS
LFKLFDEGKITQDQAIVNADSANDMRLRMNLAVVDEKAEKTDASPEPEKQSFDEDNPLAGIKLLDEDEEV

Nucleotide

• Length: 1173 bp

>NTDB_id=1096381 ACMXYN_RS14795 WP_415892496.1 3259842..3261014(+) (pilU) [Neptuniibacter sp. PT8_73]
ATGGAATTTAAGGATTACCTTACGATTTTGGCGAAACGTGACGGCTCCGACCTTTACCTAACTACCGGCGCTCCACCCTG
TGCAAAGTTCGAAGGTAAATTAACGCCATTAAGTAAAGAGCCTTATAAACCCGGTGAGATTGAAGCAATCGCAAACTCCT
TAATGGATGAGCAGCAACGCGCCACCTTCGAAGAAGAACTAGAGATGAATCTCGCTTACTCCATCCCTAAAGTGGCTCGC
TACCGAATCAATATCTTTAAACAACGTAACCAGTACTCACTAGTCGCGCGTAATATCAAAGCTGATATTCCCAAAATGGA
AGACTTAGGGCTACCTCCGGTCCTTAAAGATGTGATTATGGAGAAACGCGGCTTAGTACTGTTTGTTGGTGCGACAGGTT
CGGGTAAATCAACATCTCTTGCTGCACTGATTGATCACCGTAACCGTAATAGCGGCGGTCATATCATCACCATTGAAGAC
CCAATCGAGTTTGTCCACAACCACCGTAAATCACTGATCAATCAGCGTGAAGTCGGGGTTGATACCCGTAACTTCCACAG
CGCTCTAAAAAATGCCCTGCGCCAAGCGCCTGATGTAATTTTGATCGGTGAGATCCGAGATCAGGAAACGATGGAGCAGT
GCCTGACCTATGCAGAAACAGGTCACTTAGCTATCTCGACACTACATGCCAACAACGCTAACCAAGCGATTGATCGAATT
ATTAACTTCTTCCCACAAGCGCGGCATAAACAGATCTTCCATGACCTCGCGCTTAACCTAAAAGCCGTGGTTTCTCAGCG
CCTGATTCCAACCATTGAAGGTAAACGCTGTGCTGCCATTGAAATACTTCTAGGCACTTCCAGTATCCAAGAGCTGATCA
TGAAAGGAGATGTAAAAGGGATCAAAGAAGTTATGTCTAAAGGTTCTGATACCACCGGCATGCAAACCTTTGATGACTCC
CTATTCAAACTCTTTGACGAAGGTAAGATCACGCAAGACCAAGCTATTGTTAATGCTGACTCGGCAAACGATATGCGTCT
TCGCATGAACCTTGCGGTTGTAGATGAAAAAGCTGAAAAAACCGATGCATCTCCTGAGCCAGAAAAGCAGTCCTTCGATG
AAGACAATCCACTGGCTGGGATAAAGCTATTGGATGAAGACGAAGAGGTTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.541	90.513	0.485
pilU	Acinetobacter baylyi ADP1	51.412	90.769	0.467
pilU	Vibrio cholerae strain A1552	46.286	89.744	0.415
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.234	94.103	0.397
pilT	Pseudomonas aeruginosa PAK	42.687	85.897	0.367
pilT	Pseudomonas stutzeri DSM 10701	42.09	85.897	0.362