Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYN_RS03960 Genome accession   NZ_CP180545
Coordinates   897112..898827 (-) Length   571 a.a.
NCBI ID   WP_415893590.1    Uniprot ID   -
Organism   Neptuniibacter sp. PT8_73     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 892112..903827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYN_RS03935 (ACMXYN_03935) - 893244..894203 (+) 960 WP_415893585.1 Nudix family hydrolase -
  ACMXYN_RS03940 (ACMXYN_03940) yacG 894181..894408 (-) 228 WP_415893586.1 DNA gyrase inhibitor YacG -
  ACMXYN_RS03945 (ACMXYN_03945) coaE 894408..895010 (-) 603 WP_415893587.1 dephospho-CoA kinase -
  ACMXYN_RS03950 (ACMXYN_03950) pilD 895014..895862 (-) 849 WP_415893588.1 prepilin peptidase Machinery gene
  ACMXYN_RS03955 (ACMXYN_03955) pilC 895875..897095 (-) 1221 WP_415893589.1 type II secretion system F family protein Machinery gene
  ACMXYN_RS03960 (ACMXYN_03960) pilB 897112..898827 (-) 1716 WP_415893590.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYN_RS03965 (ACMXYN_03965) - 899066..899572 (+) 507 WP_415893591.1 pilin -
  ACMXYN_RS03970 (ACMXYN_03970) - 899754..900095 (+) 342 WP_415893592.1 hypothetical protein -
  ACMXYN_RS03975 (ACMXYN_03975) nadC 900180..901025 (-) 846 WP_415893593.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMXYN_RS03980 (ACMXYN_03980) ampD 901127..901672 (+) 546 WP_415893594.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMXYN_RS03985 (ACMXYN_03985) - 901673..902380 (-) 708 WP_415893595.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63197.98 Da        Isoelectric Point: 4.7727

>NTDB_id=1096373 ACMXYN_RS03960 WP_415893590.1 897112..898827(-) (pilB) [Neptuniibacter sp. PT8_73]
MQPSQPLSGLAKRLVNEGVCSAEVAADASLQSREKQQTFISYVIEQRLVSAYRAAAAASEEFGIPLLDLDALDPASLPQG
LINDSLITKHHALPLQKRDNRLFVAISDPTNIQALDEFKFQSGITTEAIIVEEDKLTRAIESFLEGQDSALDELEDSDLD
NLEISDGTTQDQEEQESEEDTTKDAPIVRFINKILLDSIKNGASDIHFEPYEKSYRIRSRIDGILQEVAKPPSNLAARLS
ARLKVMSQMDISEKRVPQDGRIKMKISKKRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGVEALGFAEFQKELYLGTLHR
PQGMVLVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNAKVGLNFAEALRAFLRQDPDVVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCESCKKPITLPEVTLREEG
FIDEEINNLSLFEANQDGCSKCNRGYKGRVGIYEMLPMTPEIAEVIMENGNSLDILRVAETQNFQSLRRSGLKKVAEGIT
SLEEMNRVIKT

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1096373 ACMXYN_RS03960 WP_415893590.1 897112..898827(-) (pilB) [Neptuniibacter sp. PT8_73]
TTGCAGCCAAGCCAACCTTTAAGCGGACTTGCCAAACGATTGGTTAATGAAGGCGTATGTTCTGCAGAAGTTGCAGCTGA
TGCTTCGCTTCAATCCCGAGAGAAACAACAAACGTTTATCTCATATGTTATTGAACAGCGTTTAGTTAGCGCCTACAGAG
CCGCAGCAGCTGCATCTGAAGAGTTTGGTATACCTCTTTTAGATTTGGACGCTTTAGACCCAGCATCCCTTCCTCAAGGA
CTAATTAACGATTCCCTAATAACTAAACACCATGCGCTCCCACTACAAAAACGTGATAATCGCTTGTTCGTTGCTATTTC
TGACCCGACTAATATACAAGCATTAGATGAATTTAAATTTCAAAGCGGCATTACAACAGAAGCAATCATAGTTGAGGAAG
ATAAACTCACTCGCGCCATAGAGAGCTTCCTTGAAGGCCAAGACTCAGCCTTAGATGAATTAGAAGATAGTGATTTAGAT
AATCTCGAAATTTCAGATGGCACTACTCAAGATCAAGAAGAGCAAGAAAGTGAAGAAGACACAACAAAAGATGCACCGAT
TGTTCGCTTTATCAATAAAATCCTTCTAGATTCTATAAAAAATGGCGCTTCTGATATTCATTTTGAACCCTATGAGAAAA
GCTATCGAATCAGAAGCAGGATAGATGGAATTCTGCAGGAAGTTGCAAAACCACCATCAAACCTTGCGGCACGACTATCT
GCGCGTTTAAAAGTCATGTCACAAATGGATATTTCTGAAAAACGCGTGCCGCAAGATGGCCGTATTAAGATGAAGATATC
TAAAAAACGCGCCATCGACTTCCGTGTAAATACACTCCCTACTCTTTGGGGAGAAAAAATCGTACTACGTATACTTGATC
CAAGTAGTGCAAAAATGGGGGTCGAAGCTCTAGGCTTTGCGGAGTTCCAAAAAGAGCTTTACTTAGGCACACTCCACAGA
CCGCAAGGTATGGTACTTGTAACCGGGCCAACTGGTAGTGGTAAAACAGTAAGTCTTTATACCGGTCTGAATATCCTTAA
TACCGAAGAGCGAAATATCTCTACTGCGGAAGACCCTGTAGAGATCAACCTAGAGGGTATAAACCAGGTTCATGTAAACG
CTAAAGTCGGTTTAAATTTCGCAGAGGCTTTACGCGCATTTCTTCGTCAAGACCCTGACGTGGTAATGGTAGGTGAGATA
CGAGACTTGGAAACAGCAGAAATCGCTATAAAAGCAGCCCAGACTGGTCATATGGTGCTATCCACACTTCACACAAACAG
CGCACCTGAAACACTTACCCGTCTACGTAACATGGGCGTACCCGCCTTTAATATCGCGACATCTGTTAGCCTTATCATTG
CTCAACGTCTAGGACGGCGCTTATGTGAAAGCTGCAAAAAGCCGATCACCCTCCCTGAAGTTACGTTAAGGGAAGAAGGC
TTTATAGATGAAGAGATAAACAACCTCTCTCTTTTCGAAGCCAATCAAGATGGCTGCAGCAAATGCAATAGAGGCTACAA
AGGCCGAGTGGGTATCTATGAAATGTTACCTATGACTCCAGAAATTGCAGAAGTAATTATGGAGAATGGCAACTCTCTGG
ATATACTTAGAGTAGCTGAAACACAAAACTTCCAGAGCTTACGCCGCTCTGGGTTAAAGAAAGTCGCGGAAGGAATCACC
AGTCTTGAAGAGATGAACCGAGTCATCAAGACCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.646

98.949

0.59
  pilB Acinetobacter baumannii D1279779

58.348

99.65

0.581
  pilB Legionella pneumophila strain ERS1305867

54.93

99.475

0.546
  pilB Vibrio cholerae strain A1552

55.34

90.193

0.499
  pilF Neisseria gonorrhoeae MS11

49.198

98.249

0.483
  pilB Vibrio campbellii strain DS40M4

53.488

90.368

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

53.606

89.842

0.482

Multiple sequence alignment