Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACMXYN_RS03950 Genome accession   NZ_CP180545
Coordinates   895014..895862 (-) Length   282 a.a.
NCBI ID   WP_415893588.1    Uniprot ID   -
Organism   Neptuniibacter sp. PT8_73     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 890014..900862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYN_RS03930 (ACMXYN_03930) argJ 892024..893244 (+) 1221 WP_415893584.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  ACMXYN_RS03935 (ACMXYN_03935) - 893244..894203 (+) 960 WP_415893585.1 Nudix family hydrolase -
  ACMXYN_RS03940 (ACMXYN_03940) yacG 894181..894408 (-) 228 WP_415893586.1 DNA gyrase inhibitor YacG -
  ACMXYN_RS03945 (ACMXYN_03945) coaE 894408..895010 (-) 603 WP_415893587.1 dephospho-CoA kinase -
  ACMXYN_RS03950 (ACMXYN_03950) pilD 895014..895862 (-) 849 WP_415893588.1 prepilin peptidase Machinery gene
  ACMXYN_RS03955 (ACMXYN_03955) pilC 895875..897095 (-) 1221 WP_415893589.1 type II secretion system F family protein Machinery gene
  ACMXYN_RS03960 (ACMXYN_03960) pilB 897112..898827 (-) 1716 WP_415893590.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYN_RS03965 (ACMXYN_03965) - 899066..899572 (+) 507 WP_415893591.1 pilin -
  ACMXYN_RS03970 (ACMXYN_03970) - 899754..900095 (+) 342 WP_415893592.1 hypothetical protein -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31755.71 Da        Isoelectric Point: 6.7232

>NTDB_id=1096371 ACMXYN_RS03950 WP_415893588.1 895014..895862(-) (pilD) [Neptuniibacter sp. PT8_73]
MNTIIFDPAWAIACAFLFSLCVGSFLNVVIHRLPIMMQREWEREISADEEVNQEPYNLSVPASTCPKCQHKIRWYENIPV
ISWIFLRAKCSSCGLHIPFRYPFVELFTAICSAYAVYVFGFNLIGLSVVLLTWVLVCLIFIDIDHQLLPDRLTLPLLWLG
LFVNSFGLFSSLEHAVYGAMAGYLSLWSIYWAFKLLTGKEGMGYGDFKLLAALGAWAGVYQLPLIILLSSLVGAIIGIGM
MLFKNHSSQNPIPFGPYLAIAGWIALIWGDTLTTIYLQLIRI

Nucleotide


Download         Length: 849 bp        

>NTDB_id=1096371 ACMXYN_RS03950 WP_415893588.1 895014..895862(-) (pilD) [Neptuniibacter sp. PT8_73]
ATGAATACAATTATTTTTGATCCCGCGTGGGCTATTGCCTGCGCGTTTTTATTTTCCCTTTGTGTCGGCAGCTTCTTAAA
CGTAGTCATACATCGTTTACCCATTATGATGCAAAGAGAATGGGAGAGAGAAATATCTGCTGATGAAGAGGTAAATCAAG
AGCCTTACAACCTTTCAGTACCTGCATCCACTTGCCCTAAGTGCCAACACAAAATCCGCTGGTATGAAAACATTCCTGTC
ATCAGTTGGATTTTCCTTCGGGCCAAATGTTCATCTTGTGGACTGCATATACCTTTTCGATACCCTTTTGTAGAATTATT
TACAGCTATTTGCTCCGCTTATGCCGTTTACGTTTTTGGCTTTAACCTGATTGGATTAAGCGTAGTATTACTTACATGGG
TACTGGTATGCCTGATTTTTATAGATATTGATCATCAACTACTTCCCGACCGTTTAACTCTACCTCTGTTATGGCTGGGT
TTATTCGTCAACAGTTTTGGCCTTTTCAGTTCACTAGAACACGCTGTCTATGGTGCAATGGCAGGTTACCTAAGCTTATG
GTCAATCTACTGGGCATTTAAACTGCTAACGGGCAAAGAAGGTATGGGATACGGTGATTTCAAACTACTCGCAGCCCTTG
GAGCCTGGGCTGGAGTCTATCAATTGCCGCTTATTATCCTCTTAAGCTCCCTCGTCGGTGCAATCATTGGGATAGGCATG
ATGCTATTTAAAAACCATTCATCGCAAAACCCAATTCCATTTGGCCCTTACCTCGCTATTGCGGGCTGGATAGCACTGAT
ATGGGGCGATACTCTTACGACAATCTACCTGCAACTTATAAGAATCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-118)

Type IV leader peptidase family (Peptidase_A24)

(130-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.39

95.39

0.528
  pilD Acinetobacter baumannii D1279779

55.263

94.326

0.521
  pilD Vibrio campbellii strain DS40M4

51.049

100

0.518
  pilD Acinetobacter nosocomialis M2

54.887

94.326

0.518
  pilD Neisseria gonorrhoeae MS11

46.097

95.39

0.44

Multiple sequence alignment