Detailed information NTG1096358

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMXYQ_RS14850
• Genome accession: NZ_CP180544
• Coordinates: 3254993..3256165 (+)
• Length: 390 a.a.
• NCBI ID: WP_415892496.1
• Uniprot ID: -
• Organism: Neptuniibacter sp. PT34_22
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3249993..3261165

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMXYQ_RS14830 (ACMXYQ_14830)	-	3251286..3252056 (-)	771	WP_415895149.1	metal ABC transporter permease	-
ACMXYQ_RS14835 (ACMXYQ_14835)	-	3252056..3252955 (-)	900	WP_415892491.1	metal ABC transporter substrate-binding protein	-
ACMXYQ_RS14840 (ACMXYQ_14840)	-	3252936..3254072 (-)	1137	WP_415895151.1	hypothetical protein	-
ACMXYQ_RS14845 (ACMXYQ_14845)	-	3254188..3254841 (-)	654	WP_415892494.1	DUF1826 domain-containing protein	-
ACMXYQ_RS14850 (ACMXYQ_14850)	pilU	3254993..3256165 (+)	1173	WP_415892496.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMXYQ_RS14855 (ACMXYQ_14855)	-	3256374..3256757 (+)	384	WP_415895152.1	hypothetical protein	-
ACMXYQ_RS14860 (ACMXYQ_14860)	-	3256869..3257612 (+)	744	WP_415895154.1	SDR family oxidoreductase	-
ACMXYQ_RS14865 (ACMXYQ_14865)	fadA	3257783..3258958 (-)	1176	WP_415895155.1	acetyl-CoA C-acyltransferase FadA	-
ACMXYQ_RS14870 (ACMXYQ_14870)	fadB	3258971..3261133 (-)	2163	WP_415895157.1	fatty acid oxidation complex subunit alpha FadB	-

Sequence

Protein

• Length: 390 a.a.
• Molecular weight: 43558.68 Da
• Isoelectric Point: 5.2319

>NTDB_id=1096358 ACMXYQ_RS14850 WP_415892496.1 3254993..3256165(+) (pilU) [Neptuniibacter sp. PT34_22]
MEFKDYLTILAKRDGSDLYLTTGAPPCAKFEGKLTPLSKEPYKPGEIEAIANSLMDEQQRATFEEELEMNLAYSIPKVAR
YRINIFKQRNQYSLVARNIKADIPKMEDLGLPPVLKDVIMEKRGLVLFVGATGSGKSTSLAALIDHRNRNSGGHIITIED
PIEFVHNHRKSLINQREVGVDTRNFHSALKNALRQAPDVILIGEIRDQETMEQCLTYAETGHLAISTLHANNANQAIDRI
INFFPQARHKQIFHDLALNLKAVVSQRLIPTIEGKRCAAIEILLGTSSIQELIMKGDVKGIKEVMSKGSDTTGMQTFDDS
LFKLFDEGKITQDQAIVNADSANDMRLRMNLAVVDEKAEKTDASPEPEKQSFDEDNPLAGIKLLDEDEEV

Nucleotide

• Length: 1173 bp

>NTDB_id=1096358 ACMXYQ_RS14850 WP_415892496.1 3254993..3256165(+) (pilU) [Neptuniibacter sp. PT34_22]
ATGGAATTTAAGGATTACCTTACGATTTTGGCGAAACGTGACGGCTCCGACCTTTACCTAACTACCGGCGCTCCACCCTG
TGCAAAGTTCGAAGGTAAATTAACGCCATTAAGTAAAGAGCCTTATAAACCCGGTGAGATTGAAGCAATCGCAAACTCCT
TAATGGATGAGCAGCAACGCGCCACCTTCGAAGAAGAACTAGAGATGAATCTCGCTTACTCCATCCCTAAAGTGGCTCGC
TACCGAATCAATATCTTTAAACAACGTAACCAGTACTCACTAGTCGCGCGTAATATCAAAGCTGATATTCCCAAAATGGA
AGACTTAGGGCTGCCTCCGGTCCTTAAAGATGTGATTATGGAGAAACGCGGCTTAGTACTGTTTGTTGGTGCGACAGGTT
CGGGTAAATCAACATCTCTTGCTGCACTGATTGATCACCGTAACCGTAATAGCGGCGGTCATATCATCACCATTGAAGAC
CCAATCGAGTTTGTCCACAACCACCGTAAATCACTGATCAATCAGCGTGAAGTCGGGGTTGATACCCGTAACTTCCACAG
CGCTCTAAAAAATGCCCTGCGCCAAGCGCCTGATGTAATTTTGATCGGTGAGATCCGAGATCAGGAAACGATGGAGCAGT
GCCTGACCTATGCAGAAACAGGTCACTTAGCTATCTCGACACTACATGCCAACAACGCTAACCAAGCGATTGATCGAATT
ATTAACTTCTTCCCACAAGCGCGGCATAAACAGATCTTCCATGACCTCGCGCTTAACCTAAAAGCCGTGGTTTCTCAGCG
CCTGATTCCAACCATTGAAGGTAAACGCTGTGCTGCCATTGAAATACTTCTAGGCACTTCCAGTATCCAAGAGCTGATCA
TGAAAGGAGATGTAAAAGGGATCAAAGAAGTTATGTCTAAAGGTTCTGATACCACCGGCATGCAAACCTTTGATGACTCC
CTATTCAAACTCTTTGACGAAGGTAAGATCACGCAAGACCAAGCTATTGTTAATGCTGACTCGGCAAACGATATGCGTCT
TCGCATGAACCTTGCGGTTGTAGATGAAAAAGCTGAAAAAACCGATGCATCTCCTGAGCCAGAAAAGCAGTCCTTCGATG
AAGACAATCCACTGGCTGGGATAAAGCTATTGGATGAAGACGAAGAGGTTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.541	90.513	0.485
pilU	Acinetobacter baylyi ADP1	51.412	90.769	0.467
pilU	Vibrio cholerae strain A1552	46.286	89.744	0.415
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.234	94.103	0.397
pilT	Pseudomonas aeruginosa PAK	42.687	85.897	0.367
pilT	Pseudomonas stutzeri DSM 10701	42.09	85.897	0.362