Detailed information NTG1095956

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMXD4_RS02800
• Genome accession: NZ_CP180476
• Coordinates: 616837..617994 (+)
• Length: 385 a.a.
• NCBI ID: WP_205454376.1
• Uniprot ID: -
• Organism: Methylomonas sp. TEB
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 611837..622994

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMXD4_RS02785 (ACMXD4_02785)	-	612867..613289 (-)	423	WP_415879774.1	hypothetical protein	-
ACMXD4_RS02790 (ACMXD4_02790)	-	613431..614063 (-)	633	WP_101051991.1	response regulator transcription factor	-
ACMXD4_RS02795 (ACMXD4_02795)	-	614060..616648 (-)	2589	WP_171696680.1	PAS domain S-box protein	-
ACMXD4_RS02800 (ACMXD4_02800)	pilU	616837..617994 (+)	1158	WP_205454376.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMXD4_RS02805 (ACMXD4_02805)	-	618227..618550 (-)	324	WP_415879775.1	NGG1p interacting factor NIF3	-
ACMXD4_RS02810 (ACMXD4_02810)	-	618599..618748 (-)	150	WP_165385910.1	hypothetical protein	-
ACMXD4_RS02815 (ACMXD4_02815)	lexA	618758..619387 (-)	630	WP_171696677.1	transcriptional repressor LexA	-
ACMXD4_RS02820 (ACMXD4_02820)	-	619785..620045 (-)	261	WP_171696676.1	hypothetical protein	-
ACMXD4_RS02825 (ACMXD4_02825)	-	620229..620780 (-)	552	WP_171696675.1	winged helix-turn-helix domain-containing protein	-
ACMXD4_RS02830 (ACMXD4_02830)	-	620965..621684 (-)	720	WP_033155859.1	cytochrome c1	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42362.60 Da
• Isoelectric Point: 6.3765

>NTDB_id=1095956 ACMXD4_RS02800 WP_205454376.1 616837..617994(+) (pilU) [Methylomonas sp. TEB]
MEFKDYLKILVQHDGSDLYLTAGAPPAAKFQGSLKPLENVKLSRERIKEIADGIMDAEQRAAFEQVPEMNLAITEEGIGR
FRVNIFKQRNCHALVIRNIKVDIPSADALGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDYRNSNSSGHIITIED
PIEFVHPHKKSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRPQLLMDLSLNLQAFISQRLVPTVEGKRVAAIEILLGTKLVSDLIHKGDIHAIKEAMEKSENIGMQTFDSHL
LKLYKGGVITLEEALRNSDSPNNLKLKINLSEGLGSATKKPSSLAGGLALEEITKKEGDEDSGGH

Nucleotide

• Length: 1158 bp

>NTDB_id=1095956 ACMXD4_RS02800 WP_205454376.1 616837..617994(+) (pilU) [Methylomonas sp. TEB]
ATGGAATTTAAAGACTATCTAAAAATCCTCGTCCAACACGATGGTTCGGACCTTTATCTGACGGCGGGCGCTCCACCGGC
GGCCAAGTTCCAAGGCAGCTTAAAACCGTTGGAAAACGTCAAACTTAGCCGTGAACGCATCAAGGAAATTGCTGACGGTA
TCATGGACGCCGAGCAACGCGCTGCCTTCGAACAGGTGCCGGAAATGAATCTGGCGATTACCGAAGAAGGAATCGGGCGG
TTTCGGGTGAATATTTTTAAACAGCGCAATTGCCATGCCTTAGTAATCCGCAACATCAAGGTCGATATTCCCAGTGCCGA
TGCCTTGGGCTTGCCGCAAATCCTAAAAGATAAAATCATGGAAAAGCGCGGGCTGATTTTGTTTGTCGGCGGCACCGGTT
CCGGTAAATCGACGTCCTTGGCGGCCTTGATAGATTATCGCAATAGCAACTCATCCGGGCATATCATTACCATCGAAGAT
CCTATCGAGTTTGTGCATCCGCATAAAAAATCCTTGGTCAATCAACGCGAAGTGGGTGTCGATACCCTCAGTTATGAGGA
TGCATTGAAAAACACTCTGCGGCAGGCGCCGGATGTGATTCTGATTGGCGAAATTCGCAGTCAGGAGACCATGGAACACG
CTTTGGCTTTTGCGGAAACCGGCCATCTGTGCTTGTCCACATTACACGCCAATAACGCCAATCAGGCCTTGGACCGGATC
ATCAACTTTTTCCCGGAAGAGCGCCGTCCGCAGTTGTTGATGGACTTGTCCTTGAATCTGCAAGCGTTTATTTCCCAGCG
CCTCGTGCCCACCGTGGAAGGTAAGCGGGTGGCGGCTATCGAAATTTTGCTGGGCACTAAATTGGTCAGCGATTTAATCC
ATAAAGGCGATATTCACGCCATCAAAGAAGCGATGGAAAAATCCGAAAATATCGGCATGCAGACTTTTGACAGTCATTTA
CTCAAGCTCTACAAAGGCGGCGTGATCACCTTGGAGGAGGCCTTACGCAATTCGGACTCGCCGAACAATTTAAAACTTAA
AATCAATTTGAGCGAAGGTTTGGGGTCGGCGACCAAAAAACCCAGTTCGTTGGCCGGCGGTCTTGCTTTGGAAGAAATCA
CCAAGAAGGAGGGCGATGAGGATAGTGGGGGACATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (60-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.959	100	0.561
pilU	Acinetobacter baylyi ADP1	55.367	91.948	0.509
pilU	Vibrio cholerae strain A1552	49.718	91.948	0.457
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.096	94.805	0.39
pilT	Legionella pneumophila strain Lp02	42.727	85.714	0.366
pilT	Legionella pneumophila strain ERS1305867	42.727	85.714	0.366