Detailed information NTG1095456

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACMX2G_RS01860
• Genome accession: NZ_CP180375
• Coordinates: 380733..381347 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain NJN-6
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 375733..386347

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMX2G_RS01830 (ACMX2G_01830)	rpsT	376033..376299 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACMX2G_RS01835 (ACMX2G_01835)	holA	376316..377357 (-)	1042	Protein_364	DNA polymerase III subunit delta	-
ACMX2G_RS01840 (ACMX2G_01840)	-	377397..377551 (-)	155	Protein_365	hypothetical protein	-
ACMX2G_RS01845 (ACMX2G_01845)	-	377592..377726 (+)	135	WP_003152870.1	YqzM family protein	-
ACMX2G_RS01850 (ACMX2G_01850)	comEC	377745..380096 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACMX2G_RS01855 (ACMX2G_01855)	-	380097..380666 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACMX2G_RS01860 (ACMX2G_01860)	comEA	380733..381347 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACMX2G_RS01865 (ACMX2G_01865)	comER	381406..382227 (+)	822	WP_014305449.1	late competence protein ComER	-
ACMX2G_RS01870 (ACMX2G_01870)	-	382296..383033 (-)	738	WP_024085626.1	class I SAM-dependent DNA methyltransferase	-
ACMX2G_RS01875 (ACMX2G_01875)	rsfS	383030..383386 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACMX2G_RS01880 (ACMX2G_01880)	yqeK	383404..383964 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACMX2G_RS01885 (ACMX2G_01885)	-	383954..384523 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
ACMX2G_RS01890 (ACMX2G_01890)	yhbY	384534..384824 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACMX2G_RS01895 (ACMX2G_01895)	aroE	384818..385660 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=1095456 ACMX2G_RS01860 WP_041482377.1 380733..381347(-) (comEA) [Bacillus velezensis strain NJN-6]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1095456 ACMX2G_RS01860 WP_041482377.1 380733..381347(-) (comEA) [Bacillus velezensis strain NJN-6]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363