Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJ8W9_RS07150 Genome accession   NZ_CP178751
Coordinates   1500448..1502145 (+) Length   565 a.a.
NCBI ID   WP_411360755.1    Uniprot ID   -
Organism   Pseudidiomarina sp. YC-516-91     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1495448..1507145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ8W9_RS07125 pdhR 1496141..1496896 (-) 756 WP_411359181.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACJ8W9_RS07130 ampE 1497095..1497931 (-) 837 WP_411359182.1 regulatory signaling modulator protein AmpE -
  ACJ8W9_RS07135 ampD 1497932..1498483 (-) 552 WP_411360946.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACJ8W9_RS07140 nadC 1498584..1499450 (+) 867 WP_411360753.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACJ8W9_RS07145 - 1499820..1500329 (+) 510 WP_411360754.1 pilin -
  ACJ8W9_RS07150 pilB 1500448..1502145 (+) 1698 WP_411360755.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJ8W9_RS07155 pilC 1502157..1503392 (+) 1236 WP_411360756.1 type II secretion system F family protein Machinery gene
  ACJ8W9_RS07160 - 1503394..1504281 (+) 888 WP_411360757.1 prepilin peptidase -
  ACJ8W9_RS07165 coaE 1504281..1504904 (+) 624 WP_411360758.1 dephospho-CoA kinase -
  ACJ8W9_RS07170 zapD 1504906..1505748 (+) 843 WP_411359189.1 cell division protein ZapD -
  ACJ8W9_RS07175 mutT 1505752..1506150 (-) 399 WP_411359190.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 63380.60 Da        Isoelectric Point: 5.1799

>NTDB_id=1091344 ACJ8W9_RS07150 WP_411360755.1 1500448..1502145(+) (pilB) [Pseudidiomarina sp. YC-516-91]
MLHHSLAALLLRDRRIPEAQLKSCSEYAWETKLPFTSVVIERKLIKANELAAFCYQESNCPWLDLDTFNQELIPPQFINE
RLIRKNHAVPLYQRGNVLYIAVSDPTRIETLDEFQFRTRLQAEAVLVEEDKLQRLIDKLLENETSMLGVTAEDEQELREI
DADGEVQDDKGSVSVDGKNDAPIVIYINKMLLDAIKRGASDLHFEPYEGEYRIRFRIDGVLHEIARPPYALSNRIAARLK
VMSQLDIAERRLPQDGRIKLRLSKHKAIDFRVSSLPTMYGEKIVLRILDASSAMLGIEALGYEPEQQKLYEEALQRPQGM
ILVTGPTGSGKTVSLYTGLNILNTPEVNISTAEDPVEINLYGINQVQINVKAGLTFADALRSFLRQDPDIVMVGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNSAAETLTRLMNMGVATYNIASSVSLIIAQRLARRLCNECKEEEEIPREELIRQGFKEE
ELPGLTIFKAVGCDQCTSGFKGRTGVYEVMPVTESIQELIMNNAGSLQIARQAHKDGYPSLRHSALKKVADGIISLAEAN
RVTNN

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=1091344 ACJ8W9_RS07150 WP_411360755.1 1500448..1502145(+) (pilB) [Pseudidiomarina sp. YC-516-91]
ATGCTACACCACTCCCTTGCGGCGCTTTTGCTGCGTGACAGACGAATTCCTGAAGCGCAACTTAAGAGTTGTAGCGAATA
CGCATGGGAAACCAAATTACCATTTACCAGCGTTGTTATTGAGCGCAAGCTTATCAAAGCAAATGAGCTCGCTGCTTTTT
GTTATCAAGAATCAAACTGTCCTTGGCTTGACCTTGATACTTTCAATCAAGAACTTATTCCCCCACAATTTATCAATGAG
CGGTTAATTCGCAAGAACCATGCGGTGCCTTTGTATCAGCGTGGCAATGTGCTCTATATCGCGGTATCTGATCCCACTCG
AATTGAAACCCTTGATGAGTTTCAGTTCCGTACGCGCCTGCAAGCTGAGGCGGTGTTGGTTGAAGAAGATAAGCTACAAC
GCCTTATTGATAAGTTGTTGGAAAATGAAACCTCCATGCTCGGCGTTACTGCCGAGGATGAACAAGAACTGCGTGAAATC
GATGCTGATGGCGAAGTTCAAGATGACAAAGGATCAGTAAGTGTCGATGGCAAAAACGACGCACCCATTGTTATCTACAT
CAACAAAATGCTGCTCGACGCGATTAAGCGAGGCGCTTCGGATTTGCACTTTGAGCCCTACGAAGGCGAATATCGGATTC
GTTTCCGTATTGACGGCGTATTGCACGAAATAGCACGCCCTCCTTACGCGTTGTCAAATCGTATCGCCGCACGTTTGAAG
GTTATGTCGCAGCTCGATATTGCCGAACGCCGCTTACCACAAGATGGCCGCATTAAACTGCGTTTATCCAAACATAAAGC
CATCGATTTCCGGGTTAGCTCGTTGCCGACCATGTATGGTGAAAAAATAGTGCTGCGGATTTTGGATGCCTCTTCGGCGA
TGTTGGGTATCGAAGCATTGGGTTATGAACCGGAGCAACAAAAGCTCTATGAAGAGGCGCTGCAGCGCCCGCAAGGCATG
ATTCTAGTGACCGGTCCCACGGGGTCGGGTAAAACCGTCTCACTCTATACGGGGCTGAATATTCTCAATACGCCCGAGGT
GAATATTTCAACGGCGGAAGACCCGGTTGAAATTAACTTGTACGGTATTAACCAAGTACAGATTAATGTCAAAGCCGGCT
TAACCTTTGCCGATGCTCTGCGTTCGTTTTTGCGGCAAGACCCTGACATCGTCATGGTGGGTGAGATTCGAGATCTGGAA
ACTGCAGAAATTGCCATTAAGGCGGCACAAACGGGTCACTTGGTTCTTTCGACCTTGCACACCAACTCAGCTGCGGAAAC
ACTGACACGTTTGATGAACATGGGCGTGGCCACCTACAACATCGCCAGTTCTGTGTCATTGATTATTGCCCAGCGCTTGG
CGCGACGCTTGTGTAATGAATGTAAGGAAGAAGAAGAAATTCCCCGTGAAGAACTGATTCGTCAAGGCTTCAAAGAAGAG
GAGTTACCTGGTTTAACAATCTTTAAAGCGGTCGGTTGCGACCAGTGCACCAGCGGCTTTAAAGGTCGTACAGGTGTATA
CGAAGTTATGCCGGTGACAGAATCGATTCAAGAGCTTATTATGAATAACGCAGGCTCGTTGCAAATTGCTCGACAAGCCC
ATAAAGACGGCTATCCTTCATTAAGACATTCGGCCCTGAAAAAGGTGGCTGACGGTATTATCAGTCTGGCGGAGGCGAAC
CGCGTCACTAATAACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-145)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56.028

99.823

0.559
  pilB Acinetobacter baylyi ADP1

57.195

97.168

0.556
  pilB Acinetobacter baumannii D1279779

56.014

98.584

0.552
  pilB Vibrio cholerae strain A1552

53.42

95.752

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

50

100

0.503
  pilB Vibrio campbellii strain DS40M4

55.647

86.195

0.48
  pilF Neisseria gonorrhoeae MS11

50.758

93.451

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.681

0.363

Multiple sequence alignment