Detailed information NTG1089897

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACKUFS_RS07920
• Genome accession: NZ_CP178532
• Coordinates: 1710723..1711892 (+)
• Length: 389 a.a.
• NCBI ID: WP_054080001.1
• Uniprot ID: -
• Organism: Pseudomonas cannabina strain YM7930
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1705723..1716892

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKUFS_RS07905 (ACKUFS_07905)	-	1706196..1708448 (+)	2253	WP_007249413.1	ATP-dependent DNA helicase	-
ACKUFS_RS07910 (ACKUFS_07910)	pgm	1708553..1710199 (+)	1647	WP_007249414.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
ACKUFS_RS07915 (ACKUFS_07915)	-	1710306..1710581 (-)	276	WP_007249415.1	peptidylprolyl isomerase	-
ACKUFS_RS07920 (ACKUFS_07920)	pilU	1710723..1711892 (+)	1170	WP_054080001.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACKUFS_RS07925 (ACKUFS_07925)	-	1712105..1713682 (-)	1578	WP_054080000.1	ABC transporter ATP-binding protein	-
ACKUFS_RS07930 (ACKUFS_07930)	-	1713684..1714706 (-)	1023	WP_007249418.1	ABC transporter permease	-
ACKUFS_RS07935 (ACKUFS_07935)	-	1714706..1715770 (-)	1065	WP_007249419.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42698.90 Da
• Isoelectric Point: 6.2505

>NTDB_id=1089897 ACKUFS_RS07920 WP_054080001.1 1710723..1711892(+) (pilU) [Pseudomonas cannabina strain YM7930]
MDFPALLKILANQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVASIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLEVVMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLVISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAITLTTPPPAPTGSRTASTAEWGLVDDEPGGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1089897 ACKUFS_RS07920 WP_054080001.1 1710723..1711892(+) (pilU) [Pseudomonas cannabina strain YM7930]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAATCAGGACGGATCGGACCTTTATCTTTCCACCGGCGCACCGCCGTG
CGCCAAGTTCAACGGGGTGCTCAAACCGCTGGGCAGCGAGACCTTCAAGCCCGGAGAAGTGGCGTCGATTGCCCAGGGGC
TGATGGATGAAGAGCAGAAACTCGAGTTCCTGCGCGAACTGGAAATGAACCTGGCAGTGTCGCTGGCAGGTATTGGTCGT
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCCATTGTCGCCCGTAACATCAAGCTGGATATACCGCGCTTCGA
AGACCTGTTCCTGCCGCCGGTGCTGCTCGAGGTGGTGATGGAAAAGCACGGCCTGGTGCTGTTCGTCGGTGCCACCGGGT
CGGGTAAATCGACCTCGCTGGCAGCGCTGATCGATTATCGCAACCGCAATGCCAGCGGGCATATCATCACGATTGAAGAC
CCGGTGGAGTTTATCCATCGGCACAAGAAGTCGATCGTCAATCAGCGCGAAGTCGGCGTCGATACCCGCAGCTTTCGCGC
GGCGTTGAAGAACACCCTGCGTCAGGCGCCGGACGTGATTCTGATCGGCGAAATCCGTGATCGCGAAACCATGGAACACG
CGCTGGCGTTTGCTGACACCGGGCACCTGGTGATCTCGACCCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGTATC
ATCAACTTTTTCCCGGAAGAGCGGCGTGCACAGTTACTGCATGACCTGGGTAACAACCTCAAGGCGTTCGTCTCCCAGCG
GCTGGTCAAGACCCCTGATGGCAAGCGCCGGGCAGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGATCTGATCC
AGCGTAACGAACTGACCGAACTCAAAGGCATCATGGAGAAGTCCGGCAGCCTTGGCATGCAGACGTTTGACAGCGCGTTG
TTCAATCTGGCCGTAGAGGGGGCGATCAGCGAAGAAGAAGCGCTGAAGAATGCCGACTCGCAGAACAACGTGCGGCTGCG
TCTCAAGCTGCACAGTGAAGGCGGGGCCATCACCCTGACCACGCCGCCCCCGGCACCCACCGGCAGCCGCACCGCAAGCA
CGGCCGAATGGGGGCTGGTCGACGACGAGCCGGGCGGCCCGCAAGCATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.699	99.229	0.553
pilU	Acinetobacter baylyi ADP1	51.821	91.774	0.476
pilU	Vibrio cholerae strain A1552	51.143	89.974	0.46
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.642	98.458	0.38
pilT	Pseudomonas stutzeri DSM 10701	41.399	88.175	0.365
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365