Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACL0K2_RS11010 Genome accession   NZ_CP178378
Coordinates   2420833..2422944 (-) Length   703 a.a.
NCBI ID   WP_413044398.1    Uniprot ID   -
Organism   Pseudomonas sp. YJ42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2415833..2427944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL0K2_RS10995 (ACL0K2_10995) - 2417629..2419146 (-) 1518 WP_413044396.1 SPOR domain-containing protein -
  ACL0K2_RS11000 (ACL0K2_11000) aroB 2419156..2420259 (-) 1104 WP_413044397.1 3-dehydroquinate synthase -
  ACL0K2_RS11005 (ACL0K2_11005) aroK 2420283..2420828 (-) 546 WP_239610208.1 shikimate kinase AroK -
  ACL0K2_RS11010 (ACL0K2_11010) pilQ 2420833..2422944 (-) 2112 WP_413044398.1 type IV pilus secretin PilQ Machinery gene
  ACL0K2_RS11015 (ACL0K2_11015) pilP 2422996..2423523 (-) 528 WP_413044399.1 type 4a pilus biogenesis lipoprotein PilP -
  ACL0K2_RS11020 (ACL0K2_11020) pilO 2423520..2424143 (-) 624 WP_045160491.1 type 4a pilus biogenesis protein PilO -
  ACL0K2_RS11025 (ACL0K2_11025) pilN 2424140..2424712 (-) 573 WP_045160490.1 type 4a pilus biogenesis protein PilN -
  ACL0K2_RS11030 (ACL0K2_11030) comM 2424712..2425776 (-) 1065 WP_413044400.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 703 a.a.        Molecular weight: 75700.84 Da        Isoelectric Point: 4.8905

>NTDB_id=1089236 ACL0K2_RS11010 WP_413044398.1 2420833..2422944(-) (pilQ) [Pseudomonas sp. YJ42]
MNTTLSRLGLSLLAAIISPALLAANLNSLDVASLPGDRVELKLAFDEPVPAPRGYTLDQPARIALDLPGVSSKLASKNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGSNVFVVLGENGASPAAVPAAPVAPVDRRAPVASMATGKSISNID
FRRGEDGAGNVVITLSDPSISPSIEEQGGKIRVSFAKTQLPESLRVRLDVQDFATPVKFVDSSSKADGASIAIEPSGRYD
YLAYQTDDKLTISVKPLSEADVEKRNAQHFTYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESKRQIAELAPLRREVVQVNYAKAADIAKLFQSVTAVDGQAD
ERGSIAVDDRTNNIIAYQTQERLDELRRIVTQLDIPVRQVMIEARIVEANVGYDKALGVRWGGNIVNDQWSAYGKDGARA
VDDDGILRPPGTKKIGNYTLEDGTAPVPFVDMGVLNSTSGVGIGFISDHVLLDLQLSAMEKTGNGEVVSQPKVMTADKET
AKILKGTEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFRNAVNGVPPIDKNEVNAKVLVNDG
ETVVIGGVFSNSQSQSVDKVPFLGDVPYLGRLFRRDVVTDAKSELLVFLTPRILNHQAIAVNR

Nucleotide


Download         Length: 2112 bp        

>NTDB_id=1089236 ACL0K2_RS11010 WP_413044398.1 2420833..2422944(-) (pilQ) [Pseudomonas sp. YJ42]
ATGAATACCACTCTCTCTCGTCTCGGCTTGTCTTTGCTGGCGGCGATTATTTCGCCCGCGCTGCTGGCCGCGAATCTGAA
TTCGCTCGATGTTGCGTCGCTACCCGGAGACCGTGTTGAGCTGAAACTGGCCTTCGACGAGCCGGTGCCTGCTCCGCGCG
GATACACGCTTGACCAGCCCGCTCGCATCGCCCTTGATCTGCCGGGTGTCTCCAGCAAGCTGGCGTCCAAGAACCGAGAA
CTCGGCGTTGGCAATGCGCGGAGTGTAACGGTCGTCGAGGCACAGGGCCGCACCCGGCTTATCGTCAACCTCACCTCTTT
GGTTCCCTATTCGACCCGAGTCGACGGGAGCAATGTGTTTGTCGTTCTGGGCGAAAACGGCGCATCACCCGCTGCGGTCC
CAGCCGCGCCTGTTGCACCGGTTGATAGGCGCGCGCCGGTCGCCTCAATGGCCACGGGCAAGTCGATTAGCAACATCGAT
TTTCGTCGCGGGGAAGACGGTGCCGGTAACGTAGTCATTACTCTGTCGGACCCATCGATCAGTCCGAGTATCGAGGAGCA
GGGTGGCAAGATCCGCGTATCGTTCGCCAAGACGCAATTACCGGAGTCGCTGCGGGTCAGGTTGGACGTGCAGGACTTCG
CCACGCCGGTCAAGTTTGTCGATTCGAGCAGTAAGGCCGATGGCGCAAGCATTGCGATCGAGCCTAGCGGGCGATACGAC
TATCTGGCGTACCAGACCGACGACAAGTTGACTATCAGCGTCAAGCCGCTTAGCGAAGCCGATGTCGAGAAGCGCAATGC
GCAGCATTTCACCTATTCTGGAGAGAAGCTGTCGCTGAACTTCCAAGATATCGACGTTCGTTCTGTTCTGCAGCTGATTG
CAGACTTCACTGATTTGAATCTGGTAGCGAGTGATACCGTACAGGGCAATATCACGTTGCGTTTGCAGAACGTTCCGTGG
GACCAGGCGTTGGATCTTGTCTTGAAAACCAAGGGCCTCGACAAGCGTCAGGTGGGCAACGTCCTGCTGGTTGCTCCAGC
GGACGAAATTGCTGCACGTGAACGTCAGGAGTTGGAATCCAAGCGGCAGATTGCCGAGCTGGCGCCCCTCCGTCGTGAAG
TCGTGCAGGTGAATTACGCTAAAGCTGCGGATATTGCGAAGCTTTTTCAATCGGTGACTGCAGTTGATGGGCAGGCTGAC
GAGCGTGGCTCCATCGCGGTTGATGATCGCACAAACAATATAATCGCCTACCAGACTCAAGAGCGTTTGGATGAATTACG
CCGTATCGTGACTCAGCTGGACATCCCGGTTCGTCAGGTCATGATTGAGGCTCGGATTGTTGAGGCAAACGTTGGCTATG
ATAAGGCGCTTGGGGTTCGGTGGGGCGGGAACATTGTGAACGACCAATGGAGTGCCTACGGCAAGGATGGCGCCCGAGCC
GTCGACGACGATGGGATTTTGAGGCCGCCAGGCACGAAAAAAATCGGTAATTATACTCTTGAGGACGGCACGGCGCCGGT
TCCTTTTGTCGACATGGGAGTATTAAATAGCACGTCCGGTGTAGGCATTGGTTTTATTTCAGATCACGTTCTTTTGGACC
TTCAGCTCTCTGCTATGGAGAAAACCGGAAATGGAGAAGTCGTATCCCAGCCGAAGGTGATGACCGCGGACAAAGAAACA
GCGAAGATTCTGAAAGGCACAGAAGTTCCTTATCAGGAGGCTAGCTCCAGCGGTGCGACGAGCACCTCTTTCAAAGAAGC
AGCTTTGTCCTTGGAGGTTACGCCCCAGATCACGCCGGATAACCGCATTATTATGGAAGTCAAGGTCACTAAAGATGCAC
CAGACTTCCGCAACGCAGTCAATGGCGTGCCACCAATCGATAAAAACGAAGTCAACGCCAAGGTACTGGTGAACGATGGC
GAAACAGTAGTGATCGGTGGTGTATTCTCCAACAGCCAGTCGCAAAGCGTCGACAAGGTTCCGTTTCTTGGCGACGTACC
TTATCTTGGACGGCTGTTTCGTCGTGATGTTGTGACAGATGCCAAGTCTGAGCTGTTGGTATTCCTTACCCCTAGAATCC
TGAATCACCAGGCCATTGCAGTGAATCGTTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(538-694)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)

Secretin and TonB N terminus short domain (STN)

(300-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.389

100

0.745
  comQ Acinetobacter baylyi ADP1

43.723

98.578

0.431
  pilQ Acinetobacter baumannii strain A118

40.437

100

0.421
  pilQ Acinetobacter baumannii D1279779

40.437

100

0.421

Multiple sequence alignment