Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLUR8_RS15885 Genome accession   NZ_CP178253
Coordinates   3400987..3402699 (+) Length   570 a.a.
NCBI ID   WP_001274989.1    Uniprot ID   A0A009IU58
Organism   Acinetobacter baumannii strain A118     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3395987..3407699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLUR8_RS15850 (ACLUR8_15850) nusA 3396872..3398356 (-) 1485 WP_000532248.1 transcription termination factor NusA -
  ACLUR8_RS15855 (ACLUR8_15855) rimP 3398394..3398918 (-) 525 WP_000777730.1 ribosome maturation factor RimP -
  ACLUR8_RS15875 (ACLUR8_15875) secG 3399560..3399889 (-) 330 WP_000555570.1 preprotein translocase subunit SecG -
  ACLUR8_RS15880 (ACLUR8_15880) tpiA 3399902..3400696 (-) 795 WP_000016939.1 triose-phosphate isomerase -
  ACLUR8_RS15885 (ACLUR8_15885) pilB 3400987..3402699 (+) 1713 WP_001274989.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLUR8_RS15890 (ACLUR8_15890) pilC 3402729..3403955 (+) 1227 WP_000279216.1 type II secretion system F family protein Machinery gene
  ACLUR8_RS15895 (ACLUR8_15895) pilD 3403955..3404815 (+) 861 WP_001152274.1 prepilin peptidase Machinery gene
  ACLUR8_RS15900 (ACLUR8_15900) coaE 3404817..3405413 (+) 597 WP_000874125.1 dephospho-CoA kinase -
  ACLUR8_RS15905 (ACLUR8_15905) - 3405410..3406324 (-) 915 WP_000075015.1 DMT family transporter -
  ACLUR8_RS15910 (ACLUR8_15910) rlmB 3406360..3407109 (-) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLUR8_RS15915 (ACLUR8_15915) - 3407211..3407537 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63369.74 Da        Isoelectric Point: 5.2391

>NTDB_id=1088789 ACLUR8_RS15885 WP_001274989.1 3400987..3402699(+) (pilB) [Acinetobacter baumannii strain A118]
MSALHTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVNLDGPTAQEDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1088789 ACLUR8_RS15885 WP_001274989.1 3400987..3402699(+) (pilB) [Acinetobacter baumannii strain A118]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGTTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCTAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCGTTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTGGATGTTTATGACACTTCTCAGATT
CCTAAAGATCTGGTCGATCAGAAACTAATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGGCAAATTTTATATGT
TGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGTTT
GATCTCGATGTTAATCTTGATGGCCCTACCGCTCAAGAAGATGAAGAAGAAGCTCCTCAAGGCGATGAAGCCCCGATTGT
TAAATATATTAATAAGTTATTAATTGATGCCATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCCCCTCTACAGCTTGCAAACCGCTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGTCGTATCAAGCTCAAGCTGTCAAA
AACCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTTATGGAGGCATTAGATAAACCT
CAAGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGACCCGGTAGAAATTAACCTGGAAGGAATTAATCAAGTTAACGTAAACCCCA
AGGTAGGGCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCGGCTCAAACCGGCCACATGGTGATGTCAACACTACATACTAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCGCACCCAGATTTTCGGGTTTTTCAGCCCGTAGGTTGCCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTCAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009IU58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

99.649

100

0.996
  pilB Acinetobacter baylyi ADP1

79.649

100

0.796
  pilB Legionella pneumophila strain ERS1305867

50.617

99.474

0.504
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365

Multiple sequence alignment