Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLIJP_RS18615 Genome accession   NZ_CP177354
Coordinates   4182267..4183976 (+) Length   569 a.a.
NCBI ID   WP_207780256.1    Uniprot ID   -
Organism   Pokkaliibacter plantistimulans strain L1E11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4177267..4188976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLIJP_RS18590 (ACLIJP_18590) - 4177679..4178779 (+) 1101 WP_104155601.1 extracellular solute-binding protein -
  ACLIJP_RS18595 (ACLIJP_18595) rluB 4178928..4179788 (+) 861 WP_110187300.1 23S rRNA pseudouridine(2605) synthase RluB -
  ACLIJP_RS18600 (ACLIJP_18600) - 4179861..4180487 (-) 627 WP_104155603.1 L-threonylcarbamoyladenylate synthase -
  ACLIJP_RS18605 (ACLIJP_18605) - 4180539..4181426 (-) 888 WP_110187299.1 PHP domain-containing protein -
  ACLIJP_RS18610 (ACLIJP_18610) - 4181522..4181947 (-) 426 WP_207780257.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACLIJP_RS18615 (ACLIJP_18615) pilB 4182267..4183976 (+) 1710 WP_207780256.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLIJP_RS18620 (ACLIJP_18620) pilC 4183980..4185227 (+) 1248 WP_110187298.1 type II secretion system F family protein Machinery gene
  ACLIJP_RS18625 (ACLIJP_18625) - 4185240..4186091 (+) 852 WP_110187297.1 prepilin peptidase -
  ACLIJP_RS18630 (ACLIJP_18630) coaE 4186092..4186715 (+) 624 WP_243410079.1 dephospho-CoA kinase -
  ACLIJP_RS18635 (ACLIJP_18635) yacG 4186851..4187072 (+) 222 WP_104155834.1 DNA gyrase inhibitor YacG -
  ACLIJP_RS18640 (ACLIJP_18640) - 4187091..4187471 (-) 381 WP_110187296.1 4a-hydroxytetrahydrobiopterin dehydratase -
  ACLIJP_RS18645 (ACLIJP_18645) - 4187835..4188698 (+) 864 WP_110187295.1 ribose-phosphate diphosphokinase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62597.36 Da        Isoelectric Point: 6.5118

>NTDB_id=1086818 ACLIJP_RS18615 WP_207780256.1 4182267..4183976(+) (pilB) [Pokkaliibacter plantistimulans strain L1E11]
MMGIPFQPVGLVGQLVKAGLITEAQAKEAAKGANQQQQPLPLYLVEKLGVDERALAEVISQAFSVPVLDLSKMDREVWPK
DLVKDKVIQKHYGLPLFVRGKKLFVGFCDPTQAQAISEFKFSSNMSVEPILVEAGKLAHAIETLFDSASDALAGFDDSQL
EKLEVIDELPQLNMLDLTAMTSDDAPVVKFVNKILLDAIKRGSSDIHFEPYEKIYRVRCRTDGVLHEVSRPPASLGNRIS
ARLKVMSHLDISERRLPQDGRIKLKISRTRAIDFRVNTLPTLWGEKVVLRILDPSSAQMGIDALGYDPDQKAMYLEALNQ
PQGMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINIEGINQVHINTKVGLNFAEALRSFLRQDPDVVMVGEI
RDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRMQNMGVPVYNIATSVSLIIAQRLARRLCTSCKVIEEVPAEILRDAG
FSEEQISTATIFKPVGCTRCHQGYKGRVGIYEVLKVSPRIQEVIMHNGNSLDIAAVAKAEGMSDLRQSGIKKVLSGMTSL
EELNRVIKE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1086818 ACLIJP_RS18615 WP_207780256.1 4182267..4183976(+) (pilB) [Pokkaliibacter plantistimulans strain L1E11]
ATGATGGGAATCCCCTTTCAGCCAGTAGGCTTGGTTGGCCAGCTGGTTAAAGCTGGATTAATTACAGAAGCACAAGCCAA
AGAAGCTGCCAAGGGTGCGAACCAGCAGCAGCAGCCACTGCCACTCTATTTGGTCGAAAAACTAGGAGTAGATGAGCGCG
CCTTGGCAGAGGTGATATCGCAAGCCTTCAGTGTACCCGTACTTGATTTGAGTAAGATGGATCGCGAGGTTTGGCCGAAA
GACTTGGTCAAAGACAAGGTGATCCAGAAACATTATGGGCTACCGTTATTTGTTCGCGGTAAAAAGCTGTTTGTTGGCTT
TTGTGACCCCACCCAGGCTCAGGCTATCAGTGAGTTCAAGTTCTCCAGCAATATGAGCGTTGAGCCAATACTTGTTGAGG
CAGGTAAGCTCGCTCACGCCATTGAAACCCTGTTTGACTCAGCAAGTGATGCGCTTGCCGGTTTTGATGACTCGCAGCTG
GAAAAGCTGGAAGTTATTGATGAGCTGCCTCAGCTCAATATGCTTGACCTGACAGCGATGACGTCTGACGACGCTCCAGT
GGTCAAGTTCGTCAACAAAATACTGCTGGATGCCATTAAACGTGGCTCTTCAGATATCCACTTTGAGCCTTACGAAAAAA
TCTACCGAGTACGTTGCCGTACCGATGGAGTGTTGCACGAAGTAAGTCGCCCTCCAGCATCGCTTGGCAATCGAATCTCT
GCCCGTCTTAAAGTTATGTCACACCTTGACATATCAGAACGACGTTTACCTCAGGACGGACGTATCAAACTCAAGATATC
CCGTACTCGAGCCATCGACTTCAGGGTGAATACCTTACCTACCCTTTGGGGCGAAAAGGTTGTGTTACGTATTCTGGACC
CCTCCAGTGCACAGATGGGTATTGATGCGCTGGGGTATGACCCAGACCAAAAAGCCATGTACCTGGAGGCACTCAACCAG
CCTCAGGGTATGATTCTGGTCACAGGCCCAACTGGCAGCGGCAAAACCGTATCGCTGTATACTGGCCTTAATATTCTCAA
TACTACTGAGCGTAATATTTCTACTGCAGAAGACCCGGTAGAAATAAATATTGAGGGTATTAACCAAGTTCACATTAACA
CTAAGGTAGGCCTTAACTTCGCTGAAGCGCTGAGATCCTTCTTACGTCAGGACCCTGATGTGGTGATGGTAGGTGAAATT
CGAGACCTGGAAACTGCCGAGATTGCCATCAAGGCCGCACAGACAGGCCACTTGGTACTCTCTACACTACACACTAATAG
CGCTCCAGAGACGCTAACTCGTATGCAAAATATGGGCGTGCCCGTCTATAACATTGCCACCTCTGTTAGCCTGATTATCG
CTCAGCGCCTCGCACGCCGTTTATGCACCAGCTGCAAGGTTATAGAAGAGGTACCGGCTGAAATTCTCAGAGATGCGGGT
TTTTCTGAAGAACAAATATCAACCGCAACCATATTTAAACCGGTAGGCTGTACGCGCTGCCATCAGGGCTATAAAGGCCG
TGTCGGGATATATGAAGTATTAAAGGTATCTCCCCGCATACAGGAAGTGATCATGCATAATGGCAACTCATTAGATATCG
CCGCAGTTGCCAAAGCAGAAGGAATGAGTGATCTTCGCCAGTCAGGTATCAAGAAAGTGCTGTCGGGTATGACCAGCCTG
GAAGAGCTAAACCGAGTAATCAAGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-149)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.473

98.77

0.568
  pilB Acinetobacter baylyi ADP1

56.05

98.77

0.554
  pilB Legionella pneumophila strain ERS1305867

54.724

98.594

0.54
  pilB Vibrio cholerae strain A1552

51.155

98.946

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

50.54

97.715

0.494
  pilB Vibrio campbellii strain DS40M4

49.293

99.473

0.49
  pilF Neisseria gonorrhoeae MS11

48.837

98.243

0.48
  pilF Thermus thermophilus HB27

36.285

100

0.367

Multiple sequence alignment