Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACKWD1_RS14670 Genome accession   NZ_CP177201
Coordinates   3165540..3166649 (+) Length   369 a.a.
NCBI ID   WP_109112046.1    Uniprot ID   -
Organism   Aeromonas caviae strain QZ63     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3160540..3171649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWD1_RS14635 (ACKWD1_14635) - 3160574..3161404 (+) 831 WP_201985210.1 CPBP family intramembrane glutamic endopeptidase -
  ACKWD1_RS14640 (ACKWD1_14640) - 3161479..3161898 (-) 420 WP_043153031.1 DUF4426 domain-containing protein -
  ACKWD1_RS14645 (ACKWD1_14645) yggU 3161917..3162216 (-) 300 WP_043153029.1 DUF167 family protein YggU -
  ACKWD1_RS14650 (ACKWD1_14650) - 3162216..3162767 (-) 552 WP_042014922.1 YggT family protein -
  ACKWD1_RS14655 (ACKWD1_14655) proC 3162791..3163615 (-) 825 WP_043153027.1 pyrroline-5-carboxylate reductase -
  ACKWD1_RS14660 (ACKWD1_14660) - 3163741..3164442 (-) 702 WP_049636471.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACKWD1_RS14665 (ACKWD1_14665) pilT 3164482..3165516 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  ACKWD1_RS14670 (ACKWD1_14670) pilU 3165540..3166649 (+) 1110 WP_109112046.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACKWD1_RS14675 (ACKWD1_14675) yaaA 3166682..3167455 (+) 774 WP_109112047.1 peroxide stress protein YaaA -
  ACKWD1_RS14680 (ACKWD1_14680) srmB 3167643..3168863 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  ACKWD1_RS14685 (ACKWD1_14685) - 3168991..3169701 (+) 711 WP_201972383.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ACKWD1_RS14690 (ACKWD1_14690) brnQ 3169892..3171199 (+) 1308 WP_041211055.1 branched-chain amino acid transport system II carrier protein -
  ACKWD1_RS14695 (ACKWD1_14695) - 3171289..3171516 (-) 228 WP_201985208.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40886.97 Da        Isoelectric Point: 6.1844

>NTDB_id=1086136 ACKWD1_RS14670 WP_109112046.1 3165540..3166649(+) (pilU) [Aeromonas caviae strain QZ63]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDNTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1086136 ACKWD1_RS14670 WP_109112046.1 3165540..3166649(+) (pilU) [Aeromonas caviae strain QZ63]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCTCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGACCAGCCCCCCTCGACAATACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGTTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACCGGTG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGCCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAATTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment