Detailed information NTG1080700

Bioinformatically predicted

Overview

• Name: comFC
• Type: Machinery gene
• Locus tag: ACKW6F_RS16590
• Genome accession: NZ_CP176527
• Coordinates: 3236038..3236694 (-)
• Length: 218 a.a.
• NCBI ID: WP_244948474.1
• Uniprot ID: A0A5C0WI32
• Organism: Bacillus safensis strain D-62
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3231038..3241694

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKW6F_RS16555 (ACKW6F_16555)	fliW	3231176..3231610 (-)	435	WP_024423615.1	flagellar assembly protein FliW	-
ACKW6F_RS16560 (ACKW6F_16560)	-	3231639..3232217 (-)	579	WP_024426253.1	DUF6470 family protein	-
ACKW6F_RS16565 (ACKW6F_16565)	flgL	3232264..3233172 (-)	909	WP_024423617.1	flagellar hook-associated protein FlgL	-
ACKW6F_RS16570 (ACKW6F_16570)	flgK	3233182..3234702 (-)	1521	WP_149126230.1	flagellar hook-associated protein FlgK	-
ACKW6F_RS16575 (ACKW6F_16575)	-	3234718..3235200 (-)	483	WP_149126229.1	flagellar protein FlgN	-
ACKW6F_RS16580 (ACKW6F_16580)	flgM	3235219..3235482 (-)	264	WP_024423620.1	flagellar biosynthesis anti-sigma factor FlgM	-
ACKW6F_RS16585 (ACKW6F_16585)	-	3235562..3235981 (-)	420	WP_024423621.1	TIGR03826 family flagellar region protein	-
ACKW6F_RS16590 (ACKW6F_16590)	comFC	3236038..3236694 (-)	657	WP_244948474.1	ComF family protein	Machinery gene
ACKW6F_RS16595 (ACKW6F_16595)	-	3236721..3237014 (-)	294	WP_025093967.1	late competence development ComFB family protein	-
ACKW6F_RS16600 (ACKW6F_16600)	comFA	3237063..3238460 (-)	1398	WP_276507389.1	DEAD/DEAH box helicase	Machinery gene
ACKW6F_RS16605 (ACKW6F_16605)	-	3238581..3239423 (-)	843	WP_024423625.1	DegV family protein	-
ACKW6F_RS16610 (ACKW6F_16610)	degU	3239638..3240327 (-)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
ACKW6F_RS16615 (ACKW6F_16615)	degS	3240392..3241567 (-)	1176	WP_003213166.1	sensor histidine kinase	Regulator

Sequence

Protein

• Length: 218 a.a.
• Molecular weight: 24946.05 Da
• Isoelectric Point: 9.7972

>NTDB_id=1080700 ACKW6F_RS16590 WP_244948474.1 3236038..3236694(-) (comFC) [Bacillus safensis strain D-62]
MPAFTWKSFLLLNDDHLCVACKNHLVKIERPICPTCGRAQSNDQLCQDCNRWNERSDSIKILVKNRSVYTYNELMKDVLA
RFKFRGDTALAELFQKDVQAAFKHSFCIKDPLLIPIPLSHERLKERGFNQSVLIASLLDKPILQPLVKIHQSKQSKKKKN
ERLDQKGLFQIKQTDAIVQRDIILVDDIYTTGATIYDAARILKDAGAKSVSSFTLIRS

Nucleotide

• Length: 657 bp

>NTDB_id=1080700 ACKW6F_RS16590 WP_244948474.1 3236038..3236694(-) (comFC) [Bacillus safensis strain D-62]
GTGCCTGCTTTTACATGGAAATCATTCCTACTATTAAATGATGATCACCTTTGTGTAGCATGTAAGAATCACTTAGTAAA
GATTGAGCGTCCTATTTGTCCAACATGCGGAAGGGCGCAGTCAAATGACCAATTGTGCCAGGACTGTAACAGATGGAATG
AACGCTCTGATTCTATTAAAATCCTTGTAAAAAATAGGTCAGTTTATACTTACAACGAGTTGATGAAAGATGTCCTTGCC
CGCTTTAAATTTCGGGGTGATACGGCTTTAGCTGAGCTGTTTCAAAAAGATGTACAAGCAGCATTTAAACATTCCTTCTG
CATAAAAGACCCATTGCTGATACCGATTCCTTTAAGTCATGAAAGACTAAAAGAAAGAGGATTTAATCAGTCCGTGTTAA
TCGCTTCTTTGCTAGATAAGCCAATATTGCAGCCACTCGTGAAAATACATCAAAGTAAGCAGTCTAAAAAAAAGAAAAAT
GAACGCCTAGATCAAAAAGGATTGTTTCAAATAAAGCAAACAGATGCGATTGTTCAAAGGGATATTATATTAGTTGATGA
TATCTATACAACGGGAGCAACCATCTATGATGCGGCAAGAATTTTGAAAGACGCAGGTGCTAAAAGTGTTTCTTCTTTTA
CGTTGATACGTAGTTAA

Domains

Predicted by InterproScan.

• Double zinc ribbon domain (DZR_2) (14-49)

• Phosphoribosyl transferase domain (Pribosyltran) (132-216)

3D structure

• AlphaFold DB: A0A5C0WI32

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFC	Bacillus subtilis subsp. subtilis str. 168	50	99.083	0.495
comFC/cflB	Streptococcus pneumoniae Rx1	36.744	98.624	0.362
comFC/cflB	Streptococcus pneumoniae D39	36.744	98.624	0.362
comFC/cflB	Streptococcus pneumoniae R6	36.744	98.624	0.362
comFC/cflB	Streptococcus pneumoniae TIGR4	36.744	98.624	0.362