Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACKW6F_RS16600 Genome accession   NZ_CP176527
Coordinates   3237063..3238460 (-) Length   465 a.a.
NCBI ID   WP_276507389.1    Uniprot ID   -
Organism   Bacillus safensis strain D-62     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3232063..3243460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKW6F_RS16565 (ACKW6F_16565) flgL 3232264..3233172 (-) 909 WP_024423617.1 flagellar hook-associated protein FlgL -
  ACKW6F_RS16570 (ACKW6F_16570) flgK 3233182..3234702 (-) 1521 WP_149126230.1 flagellar hook-associated protein FlgK -
  ACKW6F_RS16575 (ACKW6F_16575) - 3234718..3235200 (-) 483 WP_149126229.1 flagellar protein FlgN -
  ACKW6F_RS16580 (ACKW6F_16580) flgM 3235219..3235482 (-) 264 WP_024423620.1 flagellar biosynthesis anti-sigma factor FlgM -
  ACKW6F_RS16585 (ACKW6F_16585) - 3235562..3235981 (-) 420 WP_024423621.1 TIGR03826 family flagellar region protein -
  ACKW6F_RS16590 (ACKW6F_16590) comFC 3236038..3236694 (-) 657 WP_244948474.1 ComF family protein Machinery gene
  ACKW6F_RS16595 (ACKW6F_16595) - 3236721..3237014 (-) 294 WP_025093967.1 late competence development ComFB family protein -
  ACKW6F_RS16600 (ACKW6F_16600) comFA 3237063..3238460 (-) 1398 WP_276507389.1 DEAD/DEAH box helicase Machinery gene
  ACKW6F_RS16605 (ACKW6F_16605) - 3238581..3239423 (-) 843 WP_024423625.1 DegV family protein -
  ACKW6F_RS16610 (ACKW6F_16610) degU 3239638..3240327 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  ACKW6F_RS16615 (ACKW6F_16615) degS 3240392..3241567 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  ACKW6F_RS16620 (ACKW6F_16620) - 3241788..3242456 (+) 669 WP_012011434.1 YigZ family protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53341.31 Da        Isoelectric Point: 9.9597

>NTDB_id=1080701 ACKW6F_RS16600 WP_276507389.1 3237063..3238460(-) (comFA) [Bacillus safensis strain D-62]
MNMHKAMELMRQLHSRHLLTVETKCPQSNLDWLEEKGLVNRSPAIERKANGFTCCRCGVSHKRYFAHSPCEVCQKDCVYC
RSCIMMGKAAECGFLYEWTGPQMVETCRAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYAL
NQGMRVCIATPRTDVVLELEPRLRKAFQEVTITVLYGGSSQRFQITPLVITTTHQLMRYKNAFDVLIVDEVDAFPYSMDE
RLQFAVLKAMRKNRGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHEQPLPVPTFHWIGHWKKKLKKNQLPPKVMNWMH
RHIANKRRILLFVPSIATMKKVTKILREQSLNVEGVSADDPDRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLGS
ESTIFTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKKHIAKMNDTAAKEFSEKQCGFN

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=1080701 ACKW6F_RS16600 WP_276507389.1 3237063..3238460(-) (comFA) [Bacillus safensis strain D-62]
TTGAATATGCACAAAGCAATGGAACTAATGCGGCAGCTGCATTCACGCCATCTGCTCACCGTTGAAACAAAGTGCCCACA
GTCCAATTTAGATTGGCTGGAGGAGAAGGGCTTAGTCAATCGCTCTCCAGCCATTGAGAGGAAAGCAAATGGTTTTACAT
GCTGCCGGTGCGGTGTATCACACAAGCGGTATTTCGCTCATTCTCCATGTGAAGTGTGTCAAAAGGATTGTGTGTACTGT
AGATCATGCATCATGATGGGAAAAGCAGCAGAATGTGGGTTTCTTTATGAATGGACAGGTCCACAAATGGTAGAAACATG
TCGAGCCGAATTAACATGGCAGGGAGAGTTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGATTGAAGCTATTAAAA
ACAAATTTGATCTACTTGTTTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTGCTTTTTCACGGAATCGAATATGCGTTA
AATCAAGGAATGAGGGTCTGTATTGCGACACCTAGAACAGATGTAGTGCTTGAACTCGAACCACGGCTTAGAAAAGCATT
TCAAGAGGTGACAATTACCGTACTTTACGGAGGCAGTTCTCAAAGGTTTCAAATAACACCGCTTGTGATCACCACAACCC
ACCAGCTGATGAGATACAAAAATGCTTTTGATGTGCTCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGATGAG
CGTCTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACAGAGGGGTTAGGATTTATTTAAGTGCGACACCCTCTAAAAA
AATGACGAGAGAGGTTTCTCGTGGAAAATTGGAAGCGATAAAAATTCCTTTGCGTTTTCACGAGCAACCATTACCCGTAC
CGACCTTTCATTGGATCGGTCATTGGAAAAAGAAATTAAAAAAGAACCAGCTGCCCCCTAAAGTGATGAATTGGATGCAT
AGACATATTGCAAATAAGAGAAGAATATTACTTTTTGTTCCTTCAATTGCTACCATGAAGAAGGTAACGAAGATTCTTCG
AGAACAATCCTTAAATGTGGAGGGGGTATCTGCCGATGATCCGGATAGGAAACAAAAGGTTCAGCACTTTAGAGATTACG
TATACGATGTACTAGTTACAACCACTATTCTAGAAAGAGGCGTAACGATTCCAAATGTTCAAGTGGGGGTACTAGGTTCG
GAATCTACTATTTTTACAGAGAGTGCACTTGTTCAGATTTCTGGGCGGGTAGGCAGACACCCGGATTATTGTACAGGAGA
TGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGCAAAAATGAATGATACGGCTG
CAAAAGAGTTTTCTGAAAAACAGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(116-265)

Helicase conserved C-terminal domain (Helicase_C)

(324-420)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

52.193

98.065

0.512
  comFA/cflA Streptococcus pneumoniae Rx1

38.095

94.839

0.361
  comFA/cflA Streptococcus pneumoniae D39

38.095

94.839

0.361
  comFA/cflA Streptococcus pneumoniae R6

38.095

94.839

0.361

Multiple sequence alignment