Detailed information NTG1080623

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACJX3V_RS12380
• Genome accession: NZ_CP176523
• Coordinates: 2543227..2543841 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain MEPW12
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2538227..2548841

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJX3V_RS12350	rpsT	2538527..2538793 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACJX3V_RS12355	holA	2538810..2539851 (-)	1042	Protein_2386	DNA polymerase III subunit delta	-
ACJX3V_RS12360	-	2539891..2540045 (-)	155	Protein_2387	hypothetical protein	-
ACJX3V_RS12365	-	2540086..2540220 (+)	135	WP_003152870.1	YqzM family protein	-
ACJX3V_RS12370	comEC	2540239..2542590 (-)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACJX3V_RS12375	-	2542591..2543160 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACJX3V_RS12380	comEA	2543227..2543841 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACJX3V_RS12385	comER	2543900..2544721 (+)	822	WP_014305449.1	late competence protein ComER	-
ACJX3V_RS12390	-	2544790..2545527 (-)	738	WP_032856885.1	class I SAM-dependent DNA methyltransferase	-
ACJX3V_RS12395	rsfS	2545524..2545880 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACJX3V_RS12400	yqeK	2545898..2546458 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACJX3V_RS12405	-	2546448..2547017 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
ACJX3V_RS12410	yhbY	2547028..2547318 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACJX3V_RS12415	aroE	2547312..2548154 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=1080623 ACJX3V_RS12380 WP_003152867.1 2543227..2543841(-) (comEA) [Bacillus amyloliquefaciens strain MEPW12]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1080623 ACJX3V_RS12380 WP_003152867.1 2543227..2543841(-) (comEA) [Bacillus amyloliquefaciens strain MEPW12]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363