Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJ3Y0_RS11420 Genome accession   NZ_CP176471
Coordinates   2466653..2468338 (-) Length   561 a.a.
NCBI ID   WP_372383837.1    Uniprot ID   -
Organism   Vibrio sp. BS-M-Sm-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2461653..2473338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ3Y0_RS11395 (ACJ3Y0_11395) yacG 2462811..2463008 (-) 198 WP_327783518.1 DNA gyrase inhibitor YacG -
  ACJ3Y0_RS11400 (ACJ3Y0_11400) zapD 2463089..2463829 (-) 741 WP_086711973.1 cell division protein ZapD -
  ACJ3Y0_RS11405 (ACJ3Y0_11405) coaE 2463856..2464470 (-) 615 WP_372383836.1 dephospho-CoA kinase -
  ACJ3Y0_RS11410 (ACJ3Y0_11410) pilD 2464473..2465342 (-) 870 WP_327783519.1 A24 family peptidase Machinery gene
  ACJ3Y0_RS11415 (ACJ3Y0_11415) pilC 2465408..2466637 (-) 1230 WP_285954234.1 type II secretion system F family protein Machinery gene
  ACJ3Y0_RS11420 (ACJ3Y0_11420) pilB 2466653..2468338 (-) 1686 WP_372383837.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJ3Y0_RS11425 (ACJ3Y0_11425) - 2468338..2468802 (-) 465 WP_372383838.1 pilin -
  ACJ3Y0_RS11430 (ACJ3Y0_11430) nadC 2469055..2469945 (-) 891 WP_285954237.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACJ3Y0_RS11435 (ACJ3Y0_11435) ampD 2470029..2470627 (+) 599 Protein_2132 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACJ3Y0_RS11440 (ACJ3Y0_11440) pdhR 2471111..2471872 (+) 762 WP_017629813.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62084.89 Da        Isoelectric Point: 4.9283

>NTDB_id=1080260 ACJ3Y0_RS11420 WP_372383837.1 2466653..2468338(-) (pilB) [Vibrio sp. BS-M-Sm-2]
MLTNLPTVLRQAGLLSLTQEQAVAEHVQASGVSTPEALLVLELFTGDSLANNIKTIFGLPLVQLANVDYEVLCDQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEVSDDEMTSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMV
LMTGPTGSGKTVSLYTGLRVLNTIERNISTAEDPVEINLCGINQVQVAPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKEVDDSPDIFLRHSIPNSRT
IYKANPQGCNECNQGYSGRVGIYEVMPFTDKLKTSLISQPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1080260 ACJ3Y0_RS11420 WP_372383837.1 2466653..2468338(-) (pilB) [Vibrio sp. BS-M-Sm-2]
GTGCTAACCAATCTCCCAACCGTTCTTCGTCAGGCTGGCTTACTTAGCCTGACTCAAGAACAAGCCGTGGCGGAACACGT
ACAAGCTTCCGGTGTTTCGACACCTGAAGCTCTGCTCGTTCTTGAGCTCTTCACAGGTGATTCCTTAGCAAACAACATAA
AAACCATTTTCGGTTTGCCGTTAGTGCAGCTTGCCAATGTTGATTATGAAGTCTTGTGCGACCAATTAGGGCTTCGCGAG
CTAATCACCAAGTATCGTGCTATCCCGATTTCAGTCTCTAGCTCAACTCTCACACTCGCCTCGGCCGACCCAACCGACTT
ACAGGCGGAAGATGATTTTCGCTTTGCAACGGGATTACAGATTGAATTGGTTGTCGCTAACTACTCAGAACTAGAAGGTG
CAATACGAAAGCTGTATGGACGCTCTATTTCTGGGCAAGACTCCAAGCGTAAAGAGATCACCCAAGATGAACTCGCCAAT
CTCGTTGAAGTCTCTGACGATGAGATGACTTCAATTGAAGACCTCAGCCAAGACGATTCTCCTGTTAGCCGTTTTATCAA
TCAAATACTGGTTGATGCGGTGCGTAAAGGTGCATCCGATATCCACTTCGAACCTTATGAAGAAAACTATCGTGTGCGCT
TACGTTGCGATGGCATCCTTGTTGAAATACAACAACCGGCTTCCCATCTAAGTCGCCGTTTATCTGCTCGCTTAAAGATC
CTCGCTAAGCTGGATATTGCCGAACGTCGCTTGCCTCAAGATGGACGTATTAAGCTTCGTTTAAACGACGAACTGGCGAT
TGATATGCGTGTATCGACTCTACCGACACTGTGGGGAGAAAAGATCGTACTGCGTCTTTTAGATAGCAGCGCTGCTAATT
TAGATATCGATAAGTTGGGTTACAGCGAGGATCAAAAAGCACTCTATCTCAATGCATTGAAGCGCCCACAAGGAATGGTC
TTAATGACCGGCCCAACCGGCAGTGGTAAAACCGTTTCTCTCTACACTGGCCTTCGGGTACTTAACACCATAGAGCGCAA
CATCTCAACAGCTGAAGATCCGGTCGAGATCAATCTATGTGGTATCAATCAAGTTCAAGTCGCACCCAAAATCGGTTTTG
GGTTTGCTGAAGCATTGCGATCATTTTTACGACAAGACCCCGATGTGGTAATGGTTGGGGAGATCCGCGATTTAGAAACC
GCAGAGATCGCGATCAAAGCATCGCAAACTGGTCACTTAGTGCTTTCGACACTGCACACTAACTCTGCAGCGGAAACCGT
GACTCGACTCGCTCATATGGGGATAGAACCCTTTAACCTAGCTTCATCACTGAGTTTGATTATCGCTCAACGACTAGCAA
GACGATTGTGCAACCATTGCAAAGAAGTTGACGACTCACCGGACATATTTCTGCGTCACTCGATTCCTAACAGCCGAACC
ATCTACAAAGCTAATCCCCAAGGGTGTAATGAATGTAATCAGGGGTACTCAGGCCGAGTGGGTATCTATGAAGTGATGCC
TTTTACCGACAAGCTCAAGACCAGCCTTATCAGTCAACCCAATGCGCTAGCGATTGAAGATCTCGCGCGTCGAGAAGGCA
TGAGAACTCTGCAAGAGTCAGGGCTAGATAAATTGCTTGAAGGCACCACCAGCTATCAAGAACTGCAACGTGTTCTGTAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.404

100

0.738
  pilB Vibrio campbellii strain DS40M4

70.285

100

0.704
  pilB Vibrio parahaemolyticus RIMD 2210633

70.285

100

0.704
  pilB Acinetobacter baumannii D1279779

50.654

95.365

0.483
  pilB Legionella pneumophila strain ERS1305867

50.588

90.909

0.46
  pilB Acinetobacter baylyi ADP1

49.225

91.979

0.453
  pilF Neisseria gonorrhoeae MS11

44.107

99.822

0.44
  pilF Thermus thermophilus HB27

38.964

92.87

0.362

Multiple sequence alignment