Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJ3XG_RS11210 Genome accession   NZ_CP176468
Coordinates   2510934..2512619 (+) Length   561 a.a.
NCBI ID   WP_409438986.1    Uniprot ID   -
Organism   Psychromonas sp. GE-S-Ul-11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2505934..2517619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ3XG_RS11190 (ACJ3XG_11190) cobS 2506661..2507422 (+) 762 WP_409438982.1 adenosylcobinamide-GDP ribazoletransferase -
  ACJ3XG_RS11195 (ACJ3XG_11195) - 2507436..2508092 (+) 657 WP_409438983.1 histidine phosphatase family protein -
  ACJ3XG_RS11200 (ACJ3XG_11200) nhaD 2508366..2509808 (+) 1443 WP_409438984.1 sodium:proton antiporter NhaD -
  ACJ3XG_RS11205 (ACJ3XG_11205) - 2510505..2510927 (+) 423 WP_409438985.1 pilin -
  ACJ3XG_RS11210 (ACJ3XG_11210) pilB 2510934..2512619 (+) 1686 WP_409438986.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJ3XG_RS11215 (ACJ3XG_11215) pilC 2512771..2513997 (+) 1227 WP_290041136.1 type II secretion system F family protein Machinery gene
  ACJ3XG_RS11220 (ACJ3XG_11220) - 2514531..2515445 (-) 915 WP_409438987.1 LysR family transcriptional regulator -
  ACJ3XG_RS11225 (ACJ3XG_11225) - 2515747..2516730 (+) 984 WP_290041134.1 TRAP transporter substrate-binding protein -
  ACJ3XG_RS11230 (ACJ3XG_11230) - 2516816..2517316 (+) 501 WP_409438988.1 TRAP transporter small permease -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61660.38 Da        Isoelectric Point: 4.7504

>NTDB_id=1080200 ACJ3XG_RS11210 WP_409438986.1 2510934..2512619(+) (pilB) [Psychromonas sp. GE-S-Ul-11]
MVSNQISGLASSLAARGLLDLSKLPQITASAVSEQKALTTYLVENNYIDSVALAEFCEAEYGIPLLDSESLNLEEIPDKF
IHPKLIEKHHALPVYVQQKTLYIAMADPTNVSALEDFGFSFGMHTEALLIEETKLSEAIDAILDDGTSSLEEMGNIDDIE
VDDSESRLDEAQAVDDDAPIVKYINGVLLDAVRKGASDLHFEPYEKKYRIRFRVDGILHEFASPPVNLANRFSARLKVMS
KLDIAERRLPQDGRIKLKISNKRNIDLRVSTLPTMWGEKIVMRILDSSAANLDIDKLGYTDEQKAKYLNALAKPQGMILI
TGPTGSGKTVSLYTGLNILNTVERNISTAEDPVEINLTGINQVQINNKAGLSFASALRSFLRQDPDIVMVGEIRDLETAE
IGIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPAFNIASSVSLIIAQRLGRRLCSECKAPLEISDSELLHIGFTQEQLA
EKHNLYQPVGCSKCTDGYKGRVGIYEVMEMSDQLGHIILNDGNAMQIADIAEKEGMYTLRQSAIKQALNGITSLNEVTRI
T

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1080200 ACJ3XG_RS11210 WP_409438986.1 2510934..2512619(+) (pilB) [Psychromonas sp. GE-S-Ul-11]
ATGGTTAGTAATCAGATTAGCGGCTTGGCTTCTAGCCTAGCCGCTCGTGGTTTGCTTGACCTCAGTAAACTGCCTCAAAT
TACTGCTTCCGCAGTTTCTGAACAGAAAGCCTTAACCACCTATTTAGTTGAAAATAACTATATCGATAGTGTGGCGTTAG
CGGAGTTCTGCGAAGCGGAGTATGGTATTCCTCTATTAGACAGTGAGTCTTTAAATCTGGAAGAGATTCCTGATAAGTTT
ATTCACCCTAAACTAATAGAGAAACATCATGCCTTACCTGTGTATGTACAACAAAAAACCTTGTACATCGCCATGGCTGA
CCCGACTAATGTCTCTGCATTAGAAGACTTTGGTTTTAGCTTTGGTATGCATACTGAAGCGTTGTTAATCGAAGAAACGA
AACTTTCTGAAGCCATCGATGCCATTTTAGATGATGGGACTAGCTCCCTTGAAGAGATGGGCAACATTGACGATATTGAA
GTTGATGATTCTGAGTCTCGTTTAGATGAAGCGCAAGCCGTTGATGATGATGCGCCTATCGTAAAGTATATCAATGGTGT
GTTATTGGATGCCGTACGCAAAGGTGCATCAGATTTACATTTTGAACCCTATGAGAAAAAATATCGTATTCGTTTTCGTG
TTGACGGTATTTTACATGAGTTTGCCTCTCCTCCGGTTAACCTAGCCAATCGCTTTTCAGCGCGCTTAAAGGTAATGTCA
AAATTAGATATTGCAGAACGACGCTTACCGCAAGATGGTCGTATCAAGCTTAAAATATCCAATAAACGCAATATAGATTT
ACGTGTCAGTACGCTACCTACTATGTGGGGTGAAAAGATCGTAATGCGCATCTTAGACTCCTCAGCGGCGAATCTAGATA
TCGACAAATTGGGCTATACTGATGAGCAAAAAGCCAAGTACTTAAATGCGTTAGCTAAACCACAGGGCATGATATTAATT
ACAGGACCTACAGGAAGTGGTAAAACCGTTTCTCTATATACGGGATTAAATATTTTGAACACCGTTGAGCGAAATATTTC
TACTGCGGAAGATCCTGTGGAGATAAACTTAACCGGTATTAATCAAGTTCAGATCAACAATAAAGCGGGTTTAAGTTTTG
CTTCTGCGTTGCGCTCTTTCCTACGTCAAGATCCGGATATTGTCATGGTCGGTGAGATTCGTGATTTAGAAACTGCTGAG
ATTGGCATTAAAGCGGCACAAACCGGTCATTTAGTCTTATCTACTTTACACACAAACTCAGCTGCTGAAACTTTAACGCG
TTTATTGAACATGGGCGTACCGGCTTTTAATATTGCTTCTTCAGTGTCGTTAATTATCGCGCAGCGACTAGGTCGACGTT
TATGTAGTGAATGTAAAGCACCGCTAGAGATTTCAGACTCAGAGCTTCTGCATATTGGTTTTACTCAAGAACAATTAGCA
GAAAAACATAACCTTTATCAGCCTGTCGGCTGTTCTAAATGTACTGACGGTTACAAAGGCCGTGTCGGTATTTATGAAGT
AATGGAGATGTCTGATCAATTAGGCCATATTATTTTAAATGATGGTAATGCCATGCAAATTGCAGATATCGCAGAAAAAG
AGGGCATGTATACTCTGAGGCAATCTGCTATTAAACAAGCATTAAATGGTATCACCAGCCTTAATGAGGTCACTCGTATT
ACCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.929

99.822

0.538
  pilB Legionella pneumophila strain ERS1305867

53.097

100

0.535
  pilB Acinetobacter baumannii D1279779

56.87

93.405

0.531
  pilB Vibrio parahaemolyticus RIMD 2210633

52.197

100

0.529
  pilB Vibrio cholerae strain A1552

54.015

97.683

0.528
  pilB Vibrio campbellii strain DS40M4

51.93

100

0.528
  pilF Neisseria gonorrhoeae MS11

53.586

84.492

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

35.266

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.623

94.474

0.374
  pilF Thermus thermophilus HB27

37.112

97.504

0.362

Multiple sequence alignment