Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJU26_RS14805 Genome accession   NZ_CP175559
Coordinates   2841921..2843636 (-) Length   571 a.a.
NCBI ID   WP_414040364.1    Uniprot ID   -
Organism   Acidithiobacillus sp. M4-SHS-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2836921..2848636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJU26_RS14780 (ACJU26_14780) coaE 2837510..2838118 (-) 609 WP_414040359.1 dephospho-CoA kinase -
  ACJU26_RS14785 (ACJU26_14785) - 2838125..2838925 (-) 801 WP_414040360.1 prepilin peptidase -
  ACJU26_RS14790 (ACJU26_14790) pilR 2838922..2840274 (-) 1353 WP_414040361.1 sigma-54-dependent transcriptional regulator Regulator
  ACJU26_RS14795 (ACJU26_14795) - 2840271..2841449 (-) 1179 WP_414040362.1 two-component system sensor histidine kinase NtrB -
  ACJU26_RS14800 (ACJU26_14800) - 2841370..2841885 (-) 516 WP_414040363.1 hypothetical protein -
  ACJU26_RS14805 (ACJU26_14805) pilB 2841921..2843636 (-) 1716 WP_414040364.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJU26_RS14810 (ACJU26_14810) - 2843606..2844508 (-) 903 WP_414040365.1 shikimate dehydrogenase -
  ACJU26_RS14815 (ACJU26_14815) - 2844498..2845655 (-) 1158 WP_414040366.1 anhydro-N-acetylmuramic acid kinase -
  ACJU26_RS14820 (ACJU26_14820) - 2845790..2846272 (-) 483 WP_414040367.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  ACJU26_RS14825 (ACJU26_14825) hemH 2846326..2847339 (-) 1014 WP_414040368.1 ferrochelatase -
  ACJU26_RS14830 (ACJU26_14830) xth 2847342..2848115 (-) 774 WP_414040369.1 exodeoxyribonuclease III -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62990.82 Da        Isoelectric Point: 6.3297

>NTDB_id=1078205 ACJU26_RS14805 WP_414040364.1 2841921..2843636(-) (pilB) [Acidithiobacillus sp. M4-SHS-6]
MAANPEQIALTPVLRALVSHGLGNEVQLVHLASDPARGKKPLLFYAVEKGVVSAPALMAHFSQRYNMPMLDLEAVIIDEM
LLRRVDKHLMSRYLVLPLSKRGDTLYLAMADPTDFKAVEDIKFNTGLQVVPILVEADKLMRAVHLAHNAGTGIEDIFLDK
AKDEADADAQEEFDLSKDSDGTVEDAPVVRFVQQLLLDAIQRGVSDIHLEPYEKDLRVRYRLDGVLQDALHPPLALRDGI
TSRLKILCRLDISERRLPQDGRLRVRVPPARVIDFRVSFLPTSFGETIVLRLLDPASSKVPIEQLGFLPEQRKAFEEAIH
RPYGMILVTGPTGSGKTTTLYTALNILNTGDCNISTAEDPVEIPVYGINQVNINERIGLNFAAALRSFLRQDPDIIMVGE
VRDLETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGGVHLVMAQRLARKLCSQCKKPVRIPDQALVEA
GFHAEELPGWRPMGPVGCDACNKTGYKGRIGLYQVMPVSDAMREIIMRGGTALDLARQAASEGVLTMRQNGLRRVQEGVT
SLEEVLRVTNL

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1078205 ACJU26_RS14805 WP_414040364.1 2841921..2843636(-) (pilB) [Acidithiobacillus sp. M4-SHS-6]
ATGGCTGCAAACCCGGAACAAATTGCTTTAACGCCCGTACTGCGGGCGCTGGTCAGTCATGGGCTGGGTAATGAAGTGCA
ATTGGTGCATCTTGCCAGTGACCCCGCGCGCGGCAAGAAGCCCTTATTGTTTTATGCGGTTGAAAAAGGGGTGGTTTCGG
CCCCTGCGCTGATGGCCCATTTTTCCCAGCGCTACAACATGCCCATGCTCGATCTGGAGGCCGTCATCATTGACGAGATG
CTGCTGCGCCGGGTCGATAAGCATCTGATGAGTCGCTACCTGGTTTTGCCCTTGTCCAAGCGTGGGGACACCCTTTATCT
GGCCATGGCCGACCCCACCGATTTCAAGGCGGTGGAGGACATCAAATTCAACACCGGGTTGCAGGTGGTACCTATCCTGG
TAGAAGCCGACAAGCTGATGCGGGCGGTACATCTTGCCCATAATGCCGGCACGGGTATTGAAGATATTTTCCTGGACAAG
GCCAAGGATGAGGCCGATGCGGATGCCCAGGAAGAATTTGATCTCTCGAAGGACAGTGATGGAACCGTCGAGGATGCGCC
GGTGGTGCGTTTTGTCCAACAACTGCTGCTGGATGCCATTCAGCGGGGCGTGTCGGATATCCATCTGGAACCCTATGAAA
AGGATCTCCGGGTACGTTATCGTCTGGATGGGGTTTTGCAGGATGCGTTGCATCCACCCTTGGCTTTGCGTGATGGTATT
ACTTCCCGATTGAAGATTCTTTGTCGTCTGGATATTTCCGAACGTCGTTTACCCCAGGATGGCCGTTTGCGGGTGCGGGT
ACCCCCTGCCCGGGTCATTGATTTTCGGGTGTCTTTCCTGCCCACCAGCTTCGGAGAAACCATTGTCCTGCGCTTGCTGG
ATCCCGCCAGTTCCAAGGTACCCATAGAACAGCTCGGTTTTTTGCCGGAACAACGCAAAGCCTTTGAAGAGGCCATTCAT
CGGCCCTATGGCATGATCCTGGTGACGGGGCCTACGGGCTCGGGTAAAACCACGACGCTGTACACGGCGCTGAATATCCT
CAATACGGGTGACTGCAATATCAGTACGGCAGAAGATCCGGTGGAAATTCCGGTGTACGGGATCAATCAGGTGAATATCA
ATGAACGCATCGGTTTGAATTTTGCGGCAGCACTGCGTTCATTTTTACGCCAGGATCCGGACATCATCATGGTGGGTGAG
GTACGTGATCTGGAAACCGCAGAAACCGCCATCAAGGCGGCGCAGACCGGCCACCTGGTGCTGGCTACCCTGCACACCAA
CGACGCTCCCCAAAGTTTGACGCGTCTGGAGAACATGGGCATCCCCACCTACAATATCGCCGGCGGGGTACATTTGGTCA
TGGCCCAACGTCTGGCCCGCAAGCTCTGCAGCCAGTGTAAAAAACCGGTTCGTATTCCGGACCAGGCGCTGGTCGAGGCG
GGCTTCCATGCCGAGGAGTTGCCAGGCTGGCGGCCCATGGGACCGGTGGGTTGCGATGCGTGCAATAAAACCGGTTACAA
GGGGCGTATCGGACTCTATCAGGTCATGCCGGTCAGTGATGCCATGCGTGAAATCATCATGCGGGGAGGGACCGCCCTGG
ATCTGGCCCGACAAGCAGCCAGCGAGGGGGTGCTTACCATGCGCCAGAATGGCTTGCGCCGAGTCCAGGAAGGAGTGACG
AGCCTGGAAGAGGTGTTGCGGGTCACCAACCTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

100

0.503
  pilB Acinetobacter baylyi ADP1

48.577

98.424

0.478
  pilB Acinetobacter baumannii D1279779

49.362

96.147

0.475
  pilF Neisseria gonorrhoeae MS11

45.826

98.599

0.452
  pilB Vibrio cholerae strain A1552

45.472

92.82

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

44.508

92.469

0.412
  pilB Vibrio campbellii strain DS40M4

44.023

92.294

0.406
  pilF Thermus thermophilus HB27

38.188

98.599

0.377

Multiple sequence alignment