Detailed information NTG1078052

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACJ1CH_RS12455
• Genome accession: NZ_CP175541
• Coordinates: 2587698..2588312 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain Tcba05
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2582698..2593312

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJ1CH_RS12425 (ACJ1CH_12425)	rpsT	2582998..2583264 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACJ1CH_RS12430 (ACJ1CH_12430)	holA	2583281..2584322 (-)	1042	Protein_2401	DNA polymerase III subunit delta	-
ACJ1CH_RS12435 (ACJ1CH_12435)	-	2584362..2584516 (-)	155	Protein_2402	hypothetical protein	-
ACJ1CH_RS12440 (ACJ1CH_12440)	-	2584557..2584691 (+)	135	WP_003152870.1	YqzM family protein	-
ACJ1CH_RS12445 (ACJ1CH_12445)	comEC	2584710..2587061 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACJ1CH_RS12450 (ACJ1CH_12450)	-	2587062..2587631 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACJ1CH_RS12455 (ACJ1CH_12455)	comEA	2587698..2588312 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACJ1CH_RS12460 (ACJ1CH_12460)	comER	2588371..2589192 (+)	822	WP_014305449.1	late competence protein ComER	-
ACJ1CH_RS12465 (ACJ1CH_12465)	-	2589261..2589998 (-)	738	WP_024085626.1	class I SAM-dependent DNA methyltransferase	-
ACJ1CH_RS12470 (ACJ1CH_12470)	rsfS	2589995..2590351 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACJ1CH_RS12475 (ACJ1CH_12475)	yqeK	2590369..2590929 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACJ1CH_RS12480 (ACJ1CH_12480)	-	2590919..2591488 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
ACJ1CH_RS12485 (ACJ1CH_12485)	yhbY	2591499..2591789 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACJ1CH_RS12490 (ACJ1CH_12490)	aroE	2591783..2592625 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=1078052 ACJ1CH_RS12455 WP_041482377.1 2587698..2588312(-) (comEA) [Bacillus velezensis strain Tcba05]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1078052 ACJ1CH_RS12455 WP_041482377.1 2587698..2588312(-) (comEA) [Bacillus velezensis strain Tcba05]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363